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The map label for this gene is pilT [H]

Identifier: 167040143

GI number: 167040143

Start: 1537309

End: 1538358

Strand: Direct

Name: pilT [H]

Synonym: Teth514_1505

Alternate gene names: 167040143

Gene position: 1537309-1538358 (Clockwise)

Preceding gene: 167040142

Following gene: 167040144

Centisome position: 62.56

GC content: 38.38

Gene sequence:

>1050_bases
ATGGATACAAAGGAACTTTTAAAATTAGTAGTTGAAAGAAAAGCTTCAGACTTGCATATAACGGTTGGAGTACCTCCAGT
TCTTAGAATTAATGGCTATTTAGAAAAATTAGAAGGGGAACCTTTTACCCCTGGGCAGACTGAAGAAATAGTTAAGGATT
TATTGACAAGTGAGCAATTGAAAAAGTTAGAACAAAATGGAGATATTGATTTGTCCTATTCAGTTACAGGATTAGGAAGG
TTTAGAATAAATGTATACAAACAAAGGGGAAGTTATAGCCTTGCAATAAGGTCTGTAGCTTTGAGAATACCTACAATAGA
GGAATTGGGACTTCCTCCAATACTTAAAGACTTAGCTTTAAAGGCAAGAGGGCTTATATTGGTAACAGGACCTACTGGCA
GTGGTAAATCTACTACACTGGCTGCGATGGTGGATTGGATAAACTCTAAAAGGACTTGTCATATTCTCACATTGGAGGAC
CCTATAGAGTATTTGCATAAGCACAATAAAAGCATAGTAAATCAAAGGGAAATTGGACATGATGCAGTCTCTTATGCCTC
TGCCCTAAGAGCTGCTTTGAGGGAAGACCCAGATGTCATACTGGTAGGAGAGATGAGAGATTTAGAGACAATACAGATTG
CCATAACTGCTGCTGAGACAGGGCATCTTGTGTTATCCACCCTTCACACAATAGGAGCCGTTAAGACGATCGATAGGATT
ATTGATGTTTTTCCTCCCCATCAGCAGCAGCAGATAAAAATTCAGCTTTCAAATGTTTTAGAGGGAATTATTTCTCAACA
GCTTATACCTAAAAAGGACAACAGCGGCAGAGTAGTTGCGACAGAAGTGATGATTGCTACTCCTGCTATAAGAAATCTCA
TAAGAGAAGGGAAGACTTTCCAGATACAGTCGGCAGTTCAAACAGGTAGCAAGTTTGGAATGATGACAATGGATATGTCT
ATTTTACATCTGTTAAAAAGAGGAGTTATATCATTAGAGGATGCTTTGACATATTGCGTAGACCAAGAGAATTTTTCTCG
CATGATATAG

Upstream 100 bases:

>100_bases
ATGGCATGAAAACTTTACGAGAAAGTGCTAAAAGACTTGTATTACAAGGAAAAACTACAATTGATGAAATGATAAGACTA
ACTTACGAGGAATGAGGGAT

Downstream 100 bases:

>100_bases
AGGTGATTATATGCCGTTGTATGCTTATAGGGCAAGGGATATGGGAGGAAATCTCGTTACAGGGACTTTGGAAGTTGACT
CAAAATCTCAGTGTATTGAC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MDTKELLKLVVERKASDLHITVGVPPVLRINGYLEKLEGEPFTPGQTEEIVKDLLTSEQLKKLEQNGDIDLSYSVTGLGR
FRINVYKQRGSYSLAIRSVALRIPTIEELGLPPILKDLALKARGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLED
PIEYLHKHNKSIVNQREIGHDAVSYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
IDVFPPHQQQQIKIQLSNVLEGIISQQLIPKKDNSGRVVATEVMIATPAIRNLIREGKTFQIQSAVQTGSKFGMMTMDMS
ILHLLKRGVISLEDALTYCVDQENFSRMI

Sequences:

>Translated_349_residues
MDTKELLKLVVERKASDLHITVGVPPVLRINGYLEKLEGEPFTPGQTEEIVKDLLTSEQLKKLEQNGDIDLSYSVTGLGR
FRINVYKQRGSYSLAIRSVALRIPTIEELGLPPILKDLALKARGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLED
PIEYLHKHNKSIVNQREIGHDAVSYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
IDVFPPHQQQQIKIQLSNVLEGIISQQLIPKKDNSGRVVATEVMIATPAIRNLIREGKTFQIQSAVQTGSKFGMMTMDMS
ILHLLKRGVISLEDALTYCVDQENFSRMI
>Mature_349_residues
MDTKELLKLVVERKASDLHITVGVPPVLRINGYLEKLEGEPFTPGQTEEIVKDLLTSEQLKKLEQNGDIDLSYSVTGLGR
FRINVYKQRGSYSLAIRSVALRIPTIEELGLPPILKDLALKARGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLED
PIEYLHKHNKSIVNQREIGHDAVSYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
IDVFPPHQQQQIKIQLSNVLEGIISQQLIPKKDNSGRVVATEVMIATPAIRNLIREGKTFQIQSAVQTGSKFGMMTMDMS
ILHLLKRGVISLEDALTYCVDQENFSRMI

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=328, Percent_Identity=46.3414634146341, Blast_Score=297, Evalue=8e-82,
Organism=Escherichia coli, GI1789723, Length=224, Percent_Identity=40.625, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786296, Length=384, Percent_Identity=31.5104166666667, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38671; Mature: 38671

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTKELLKLVVERKASDLHITVGVPPVLRINGYLEKLEGEPFTPGQTEEIVKDLLTSEQL
CCHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
KKLEQNGDIDLSYSVTGLGRFRINVYKQRGSYSLAIRSVALRIPTIEELGLPPILKDLAL
HHHHCCCCEEEEEEEEEEEEEEEEEEECCCCEEEEEEEHHEECCCHHHHCCCHHHHHHHH
KARGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLEDPIEYLHKHNKSIVNQREIGH
HHCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCHHHHHHHHCCH
DAVSYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
IDVFPPHQQQQIKIQLSNVLEGIISQQLIPKKDNSGRVVATEVMIATPAIRNLIREGKTF
HHCCCCCCCHHHEEHHHHHHHHHHHHCCCCCCCCCCCEEEEHEEHHCHHHHHHHHCCCCE
QIQSAVQTGSKFGMMTMDMSILHLLKRGVISLEDALTYCVDQENFSRMI
EEHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure
MDTKELLKLVVERKASDLHITVGVPPVLRINGYLEKLEGEPFTPGQTEEIVKDLLTSEQL
CCHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
KKLEQNGDIDLSYSVTGLGRFRINVYKQRGSYSLAIRSVALRIPTIEELGLPPILKDLAL
HHHHCCCCEEEEEEEEEEEEEEEEEEECCCCEEEEEEEHHEECCCHHHHCCCHHHHHHHH
KARGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLEDPIEYLHKHNKSIVNQREIGH
HHCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCHHHHHHHHCCH
DAVSYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
IDVFPPHQQQQIKIQLSNVLEGIISQQLIPKKDNSGRVVATEVMIATPAIRNLIREGKTF
HHCCCCCCCHHHEEHHHHHHHHHHHHCCCCCCCCCCCEEEEHEEHHCHHHHHHHHCCCCE
QIQSAVQTGSKFGMMTMDMSILHLLKRGVISLEDALTYCVDQENFSRMI
EEHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]