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The map label for this gene is aslB [C]

Identifier: 167040100

GI number: 167040100

Start: 1492211

End: 1493596

Strand: Direct

Name: aslB [C]

Synonym: Teth514_1460

Alternate gene names: 167040100

Gene position: 1492211-1493596 (Clockwise)

Preceding gene: 167040099

Following gene: 167040101

Centisome position: 60.73

GC content: 32.32

Gene sequence:

>1386_bases
ATGTCAGAGCTAATGCATAAATTTAAAAGACTGGGAATGAATATTGTAGTAGATCCGGTAAGTGGCTCTATCCATGTGGT
AGACGATATTATTTATGATATTTTGGACTTTTATGAAAATCGCTCAAAAGAAGAAATTGTAAATTTACTTCAGCATAAAT
ATAAAAAAGAAGATATACTGGAAGCTATTGCAGAAATTGATGAATTAAAAGAAAAAGGGTTATTATTTTCAGAAGATATA
TATAAAGATATTCCAGTGTCGAGAAATGATTCTGTTATAAAAGCGATGTGTTTGAATGTTGCTCACGATTGCAATTTAAG
GTGCAAATATTGTTTTGCTTCTACTGGGGATTTTAAAGGTAGCCGCAAGTTGATGGATTTTGAGACGGGCAAAAAAGCAA
TTGATTTTTTAATAAAAAGTTCTGGGAAAAGGCGAAACATTGAAGTTGATTTTTTTGGCGGTGAACCCCTTTTAAATTTT
GAGGTAGTCAAGCGGTTAGTAGAATATGGAAAAGAAAAGGCGAAAGAAAATAAAAAAGTAATTAAATTTACCATAACAAC
CAATGCGGTTTTGCTGGATGATGAGAAAATAAAGTATTTTAATGAAAATTTTTCTAATGTGGTACTTAGTTTGGATGGAA
GAAAAGAAGTAAATGACAGCATGAGGATACGTGTAGACGGCAGTGGAAGTTATGATACAATAGTTCCAAAAATAAAAAAA
TTCGTTGAATCAAGAGGGAAAAAAGAATACTATGTGAGAGGGACTTTTACAGCAAAAAATTTGGATTTTGCTAATGATGT
GTTGCATATTGCTGATTTGGGAGTTTATGAAATATCTGTAGAGCCGGTAGTTGAAAAAGAAGATAAAGACTATACATTAA
AGGAAGAGCATTTAGAGAGAATTTTAAATGAATATGACAGATTAGCAGAAGAATATGTAAAGAGATACGAAGAAGGAAGG
CCTTTTTCTTTTTATCATTTTAAAATAGATTTAGAAGGAGGGCCTTGCATTAAAAAAAGACTTCAAGGCTGTGGTGCTGG
TTTTGAGTATATAGCGGTGACTCCCGATGGGGATATTTATCCTTGCCATCAATTTGTGGGTATTGAGGAATTTAAATTAG
GGAATCTTGAAGAAGGTATAACTAATAAAGAATTACAGCAAAAATTTATGGAAAGCGATATTTACAAAAAAGAAGAATGT
ACGAAATGTTGGGCGAGGTTCTATTGCAGTGGAGGTTGCTTTGCAAATAACTACAACATAAATGGAGATATTAATAAACC
TTACGAGCTGGCTTGTGAAATGCAAAAAAGGCGTTTTGAAAATGCGATTGCCATAAAAGCATATCAAATGCTAAAGGGTG
AGGAAAATGATAATAAAGGAATATAA

Upstream 100 bases:

>100_bases
TTGTACTGTAGCAAACCAAGAGTGTCAATATTAATACATATTACTCAAATGGCAGTAAAGTGTAATTACTGCCATTTAAA
CTTGCAATGGAGGTTAAAAA

Downstream 100 bases:

>100_bases
AAGTATGAAGCCCAAAATAGATGATGAGGCGTATATAGCGGAAACAGCAGAGGTTATTGGAGATGTAGAAATAAAAAAAG
ATGCAAATATATGGTATGGG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: AnSME; Cys-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSELMHKFKRLGMNIVVDPVSGSIHVVDDIIYDILDFYENRSKEEIVNLLQHKYKKEDILEAIAEIDELKEKGLLFSEDI
YKDIPVSRNDSVIKAMCLNVAHDCNLRCKYCFASTGDFKGSRKLMDFETGKKAIDFLIKSSGKRRNIEVDFFGGEPLLNF
EVVKRLVEYGKEKAKENKKVIKFTITTNAVLLDDEKIKYFNENFSNVVLSLDGRKEVNDSMRIRVDGSGSYDTIVPKIKK
FVESRGKKEYYVRGTFTAKNLDFANDVLHIADLGVYEISVEPVVEKEDKDYTLKEEHLERILNEYDRLAEEYVKRYEEGR
PFSFYHFKIDLEGGPCIKKRLQGCGAGFEYIAVTPDGDIYPCHQFVGIEEFKLGNLEEGITNKELQQKFMESDIYKKEEC
TKCWARFYCSGGCFANNYNINGDINKPYELACEMQKRRFENAIAIKAYQMLKGEENDNKGI

Sequences:

>Translated_461_residues
MSELMHKFKRLGMNIVVDPVSGSIHVVDDIIYDILDFYENRSKEEIVNLLQHKYKKEDILEAIAEIDELKEKGLLFSEDI
YKDIPVSRNDSVIKAMCLNVAHDCNLRCKYCFASTGDFKGSRKLMDFETGKKAIDFLIKSSGKRRNIEVDFFGGEPLLNF
EVVKRLVEYGKEKAKENKKVIKFTITTNAVLLDDEKIKYFNENFSNVVLSLDGRKEVNDSMRIRVDGSGSYDTIVPKIKK
FVESRGKKEYYVRGTFTAKNLDFANDVLHIADLGVYEISVEPVVEKEDKDYTLKEEHLERILNEYDRLAEEYVKRYEEGR
PFSFYHFKIDLEGGPCIKKRLQGCGAGFEYIAVTPDGDIYPCHQFVGIEEFKLGNLEEGITNKELQQKFMESDIYKKEEC
TKCWARFYCSGGCFANNYNINGDINKPYELACEMQKRRFENAIAIKAYQMLKGEENDNKGI
>Mature_460_residues
SELMHKFKRLGMNIVVDPVSGSIHVVDDIIYDILDFYENRSKEEIVNLLQHKYKKEDILEAIAEIDELKEKGLLFSEDIY
KDIPVSRNDSVIKAMCLNVAHDCNLRCKYCFASTGDFKGSRKLMDFETGKKAIDFLIKSSGKRRNIEVDFFGGEPLLNFE
VVKRLVEYGKEKAKENKKVIKFTITTNAVLLDDEKIKYFNENFSNVVLSLDGRKEVNDSMRIRVDGSGSYDTIVPKIKKF
VESRGKKEYYVRGTFTAKNLDFANDVLHIADLGVYEISVEPVVEKEDKDYTLKEEHLERILNEYDRLAEEYVKRYEEGRP
FSFYHFKIDLEGGPCIKKRLQGCGAGFEYIAVTPDGDIYPCHQFVGIEEFKLGNLEEGITNKELQQKFMESDIYKKEECT
KCWARFYCSGGCFANNYNINGDINKPYELACEMQKRRFENAIAIKAYQMLKGEENDNKGI

Specific function: Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemical mechanism initiated via the reduc

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994973, Length=376, Percent_Identity=24.2021276595745, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53317; Mature: 53186

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELMHKFKRLGMNIVVDPVSGSIHVVDDIIYDILDFYENRSKEEIVNLLQHKYKKEDIL
CHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHH
EAIAEIDELKEKGLLFSEDIYKDIPVSRNDSVIKAMCLNVAHDCNLRCKYCFASTGDFKG
HHHHHHHHHHHCCCEECHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
SRKLMDFETGKKAIDFLIKSSGKRRNIEVDFFGGEPLLNFEVVKRLVEYGKEKAKENKKV
CCEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEE
IKFTITTNAVLLDDEKIKYFNENFSNVVLSLDGRKEVNDSMRIRVDGSGSYDTIVPKIKK
EEEEEECCEEEECCCHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHH
FVESRGKKEYYVRGTFTAKNLDFANDVLHIADLGVYEISVEPVVEKEDKDYTLKEEHLER
HHHCCCCCEEEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHH
ILNEYDRLAEEYVKRYEEGRPFSFYHFKIDLEGGPCIKKRLQGCGAGFEYIAVTPDGDIY
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCEE
PCHQFVGIEEFKLGNLEEGITNKELQQKFMESDIYKKEECTKCWARFYCSGGCFANNYNI
CHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCC
NGDINKPYELACEMQKRRFENAIAIKAYQMLKGEENDNKGI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SELMHKFKRLGMNIVVDPVSGSIHVVDDIIYDILDFYENRSKEEIVNLLQHKYKKEDIL
HHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHH
EAIAEIDELKEKGLLFSEDIYKDIPVSRNDSVIKAMCLNVAHDCNLRCKYCFASTGDFKG
HHHHHHHHHHHCCCEECHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
SRKLMDFETGKKAIDFLIKSSGKRRNIEVDFFGGEPLLNFEVVKRLVEYGKEKAKENKKV
CCEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEE
IKFTITTNAVLLDDEKIKYFNENFSNVVLSLDGRKEVNDSMRIRVDGSGSYDTIVPKIKK
EEEEEECCEEEECCCHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHH
FVESRGKKEYYVRGTFTAKNLDFANDVLHIADLGVYEISVEPVVEKEDKDYTLKEEHLER
HHHCCCCCEEEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHH
ILNEYDRLAEEYVKRYEEGRPFSFYHFKIDLEGGPCIKKRLQGCGAGFEYIAVTPDGDIY
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCEE
PCHQFVGIEEFKLGNLEEGITNKELQQKFMESDIYKKEECTKCWARFYCSGGCFANNYNI
CHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCC
NGDINKPYELACEMQKRRFENAIAIKAYQMLKGEENDNKGI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA