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The map label for this gene is tgt [H]

Identifier: 167040097

GI number: 167040097

Start: 1490045

End: 1491172

Strand: Direct

Name: tgt [H]

Synonym: Teth514_1457

Alternate gene names: 167040097

Gene position: 1490045-1491172 (Clockwise)

Preceding gene: 167040096

Following gene: 167040098

Centisome position: 60.64

GC content: 35.46

Gene sequence:

>1128_bases
ATGGCAGCGATTAAATATCGGCTCATCAAAAAAGATAGCAGAACAAATGCGAGATTAGGAATATTAGAGACTCCCCATGG
TGTTATAGAAACGCCTGTGTTTATGCCAGTAGGAACACAAGCAACTGTAAAAGCAATGACTCCAGAAGAATTAAAAGAAA
TTGGAGCGACAATTATTCTAAGTAATACTTATCACCTCTATTTAAGGCCAGGACATAAAATTATTGAAAAAGCTGGTGGA
CTTCACAGATTTATGAATTGGGATAGGGCAATTTTAACAGACAGTGGAGGATTTCAAATTTTTAGTTTAAGTTCTTTGAG
GAAAATTAAAGAAGAGGGAGTAGAGTTTAGGTCTCATATAGATGGTTCTAAACACTTTTTTACTCCTGAAAAAGTAATAG
AAATACAAAATGCCTTAGGTTCGGATATAATAATGTCTTTTGACGAGTGTGCACCTTATCCTGCAGATTATGAGTATGTC
AAAAATTCTATGGAGCTTACCATCAAATGGGCTGAAAGAGGAAAAAAAGCTCATAAAAATACTGAAAAACAAGCTCTTTT
TGGCATTGTCCAAGGAGGGATTTATGAAGATTTAAGAAAAGAATGCGCTCAAAGATTAGTAGAGATGGACTTTCCTGGAT
ACTCTATAGGTGGATTAAGTGTAGGAGAACCAAAGAATGTAATGTACGACATTGTAGATTTAACTACAGAATATTTACCT
GAAAACAAACCGAGATATCTTATGGGGGTGGGAAGTCCTGATGACCTTATAGAGGGAGTAATACGAGGAGTTGATATGTT
TGATTGCGTACTTCCGACGAGAATTGCTAGAAATGGGACAGTTTTCACAAGCAAGGGCAAATTAATAGTAAGAGATGCTC
CTTATGCGGAGGACTTTTCTCCTTTAGACGAAGAGTGTGATTGTTATACTTGCAAAAATTATTCAAGAGCCTATATAAGG
CATTTGTTTAAGGCTAATGAAATTTTAGCAGCAAGGCTTGCAACTATACACAACCTATATTTCCTTATTAAATTGATGGA
GAGGATAAGGGAAGCTATAAGGCAAGACCGGCTGCTTGAATTTAAAAAACAATTTTTTAAAAAATATGGTTATAAGGAGG
AGTATTGA

Upstream 100 bases:

>100_bases
AAACATAATGAGGGCTTATAAAATAGCCGTTGAAAAGAAATATAGATTTTTTAGCTTTGGAGATGCAATGCTTATAATTT
GAGCAGAATAGGAGGATATA

Downstream 100 bases:

>100_bases
TGAATCCCGAAACGACTTACGTAATTGTTCAGATGCTTATTTTTGTTGCAATCTTTTACTTTTTATTGATTTTACCTCAA
CAAAGGCGACAAAAGAAAGA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MAAIKYRLIKKDSRTNARLGILETPHGVIETPVFMPVGTQATVKAMTPEELKEIGATIILSNTYHLYLRPGHKIIEKAGG
LHRFMNWDRAILTDSGGFQIFSLSSLRKIKEEGVEFRSHIDGSKHFFTPEKVIEIQNALGSDIIMSFDECAPYPADYEYV
KNSMELTIKWAERGKKAHKNTEKQALFGIVQGGIYEDLRKECAQRLVEMDFPGYSIGGLSVGEPKNVMYDIVDLTTEYLP
ENKPRYLMGVGSPDDLIEGVIRGVDMFDCVLPTRIARNGTVFTSKGKLIVRDAPYAEDFSPLDEECDCYTCKNYSRAYIR
HLFKANEILAARLATIHNLYFLIKLMERIREAIRQDRLLEFKKQFFKKYGYKEEY

Sequences:

>Translated_375_residues
MAAIKYRLIKKDSRTNARLGILETPHGVIETPVFMPVGTQATVKAMTPEELKEIGATIILSNTYHLYLRPGHKIIEKAGG
LHRFMNWDRAILTDSGGFQIFSLSSLRKIKEEGVEFRSHIDGSKHFFTPEKVIEIQNALGSDIIMSFDECAPYPADYEYV
KNSMELTIKWAERGKKAHKNTEKQALFGIVQGGIYEDLRKECAQRLVEMDFPGYSIGGLSVGEPKNVMYDIVDLTTEYLP
ENKPRYLMGVGSPDDLIEGVIRGVDMFDCVLPTRIARNGTVFTSKGKLIVRDAPYAEDFSPLDEECDCYTCKNYSRAYIR
HLFKANEILAARLATIHNLYFLIKLMERIREAIRQDRLLEFKKQFFKKYGYKEEY
>Mature_374_residues
AAIKYRLIKKDSRTNARLGILETPHGVIETPVFMPVGTQATVKAMTPEELKEIGATIILSNTYHLYLRPGHKIIEKAGGL
HRFMNWDRAILTDSGGFQIFSLSSLRKIKEEGVEFRSHIDGSKHFFTPEKVIEIQNALGSDIIMSFDECAPYPADYEYVK
NSMELTIKWAERGKKAHKNTEKQALFGIVQGGIYEDLRKECAQRLVEMDFPGYSIGGLSVGEPKNVMYDIVDLTTEYLPE
NKPRYLMGVGSPDDLIEGVIRGVDMFDCVLPTRIARNGTVFTSKGKLIVRDAPYAEDFSPLDEECDCYTCKNYSRAYIRH
LFKANEILAARLATIHNLYFLIKLMERIREAIRQDRLLEFKKQFFKKYGYKEEY

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=360, Percent_Identity=47.7777777777778, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI13375872, Length=291, Percent_Identity=24.7422680412371, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786607, Length=368, Percent_Identity=55.1630434782609, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=47.1751412429379, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI25146502, Length=182, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920510, Length=366, Percent_Identity=46.1748633879781, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI21355311, Length=161, Percent_Identity=32.9192546583851, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42930; Mature: 42799

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIKYRLIKKDSRTNARLGILETPHGVIETPVFMPVGTQATVKAMTPEELKEIGATIIL
CCCEEEEHECCCCCCCCEEEEEECCCCCCCCCEEEECCCCCEEECCCHHHHHHCCCEEEE
SNTYHLYLRPGHKIIEKAGGLHRFMNWDRAILTDSGGFQIFSLSSLRKIKEEGVEFRSHI
ECCEEEEECCCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHC
DGSKHFFTPEKVIEIQNALGSDIIMSFDECAPYPADYEYVKNSMELTIKWAERGKKAHKN
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEHHCCCHHHCC
TEKQALFGIVQGGIYEDLRKECAQRLVEMDFPGYSIGGLSVGEPKNVMYDIVDLTTEYLP
HHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
ENKPRYLMGVGSPDDLIEGVIRGVDMFDCVLPTRIARNGTVFTSKGKLIVRDAPYAEDFS
CCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCC
PLDEECDCYTCKNYSRAYIRHLFKANEILAARLATIHNLYFLIKLMERIREAIRQDRLLE
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKQFFKKYGYKEEY
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
AAIKYRLIKKDSRTNARLGILETPHGVIETPVFMPVGTQATVKAMTPEELKEIGATIIL
CCEEEEHECCCCCCCCEEEEEECCCCCCCCCEEEECCCCCEEECCCHHHHHHCCCEEEE
SNTYHLYLRPGHKIIEKAGGLHRFMNWDRAILTDSGGFQIFSLSSLRKIKEEGVEFRSHI
ECCEEEEECCCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHC
DGSKHFFTPEKVIEIQNALGSDIIMSFDECAPYPADYEYVKNSMELTIKWAERGKKAHKN
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEHHCCCHHHCC
TEKQALFGIVQGGIYEDLRKECAQRLVEMDFPGYSIGGLSVGEPKNVMYDIVDLTTEYLP
HHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
ENKPRYLMGVGSPDDLIEGVIRGVDMFDCVLPTRIARNGTVFTSKGKLIVRDAPYAEDFS
CCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCC
PLDEECDCYTCKNYSRAYIRHLFKANEILAARLATIHNLYFLIKLMERIREAIRQDRLLE
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKQFFKKYGYKEEY
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA