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The map label for this gene is queA [H]

Identifier: 167040096

GI number: 167040096

Start: 1489001

End: 1490026

Strand: Direct

Name: queA [H]

Synonym: Teth514_1456

Alternate gene names: 167040096

Gene position: 1489001-1490026 (Clockwise)

Preceding gene: 167040095

Following gene: 167040097

Centisome position: 60.6

GC content: 32.94

Gene sequence:

>1026_bases
ATGAAGCGCAGTGAGTTTTACTTTGAACTACCAGAAGAATTAATTGCACAAGAACCTTTAGAAGACAGAGCAAGTTCTCG
ATTAATGATTTTAAATAAAAAAACCGGTGAAATAGAACATGATATTTTTAAAAACATAACAAAATATTTAAAACCAGGGG
ATTGTTTAGTACTCAATAACACTAAAGTAATTCCTGCACGCCTTATTGGAAGAAGAGAAGACTCGGGTGGAAAAATAGAA
TTTGTTCTTTTAAAAAGAATATCCAATAATGAATGGGAAATATTAGTAAAACCCGGAAGGCGAGCCAAAATAGGGTCAAA
ATTTGTGTTTGGAAATGGAGAGTTAATAGCAGAAATTTTGGATACTACAGAAGTAGGGGGAAGGATAGTAAGATTTTATT
ATGAAGGAGTATTTGAAGAAGTATTGGATAAATTAGGCGAAATGCCTGTTCCCCCTTATATCAAGAAAAAATTAAAAAAT
AAAGACAGATACCAAACTGTTTACGCAAAATATGAAGGTTCTGCAGCTGCTCCTACTGCAGGGCTTCATTTCACAGAAGA
GTTATTAGAAAAAATAAGGCAAATGGGAGTAAAAACTGTTTTTATTACTCTTCACGTAGGGTTAGGTACTTTTAGACCTG
TAAAGGAAGAAGTAATTGAAAATCATAAAATGCATGAAGAATTTTACATTGTTACAAAAGAAGCAGCTGAGGAAATAAAT
GAGACTAGGAAAAGTGGAGGAAGAATCATAGCAGTCGGTACGACTTCTACTCGAACTCTTGAAACAGTTGCTGATGAAGA
TGGTTATATAAAAGAAAAAAGTGGTTGGACTGATATTTTTATATATCCTGGTTACAAATTTAAAGCTATTGACGGCATGA
TAACAAATTTTCACTTACCTGAGTCTACTCTAATTATGATGGTGTCAGCTTTTGCTGGTAAGGAAAACATAATGAGGGCT
TATAAAATAGCCGTTGAAAAGAAATATAGATTTTTTAGCTTTGGAGATGCAATGCTTATAATTTGA

Upstream 100 bases:

>100_bases
TTAAAGGTATAATCAGTACCATGTTCGTATGCTATAGATTTATAAAATAAGTTTTTTATGGACAAATTATAAACAAAACA
ATAAAAGGATGGAGTAAGAT

Downstream 100 bases:

>100_bases
GCAGAATAGGAGGATATAATGGCAGCGATTAAATATCGGCTCATCAAAAAAGATAGCAGAACAAATGCGAGATTAGGAAT
ATTAGAGACTCCCCATGGTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKRSEFYFELPEELIAQEPLEDRASSRLMILNKKTGEIEHDIFKNITKYLKPGDCLVLNNTKVIPARLIGRREDSGGKIE
FVLLKRISNNEWEILVKPGRRAKIGSKFVFGNGELIAEILDTTEVGGRIVRFYYEGVFEEVLDKLGEMPVPPYIKKKLKN
KDRYQTVYAKYEGSAAAPTAGLHFTEELLEKIRQMGVKTVFITLHVGLGTFRPVKEEVIENHKMHEEFYIVTKEAAEEIN
ETRKSGGRIIAVGTTSTRTLETVADEDGYIKEKSGWTDIFIYPGYKFKAIDGMITNFHLPESTLIMMVSAFAGKENIMRA
YKIAVEKKYRFFSFGDAMLII

Sequences:

>Translated_341_residues
MKRSEFYFELPEELIAQEPLEDRASSRLMILNKKTGEIEHDIFKNITKYLKPGDCLVLNNTKVIPARLIGRREDSGGKIE
FVLLKRISNNEWEILVKPGRRAKIGSKFVFGNGELIAEILDTTEVGGRIVRFYYEGVFEEVLDKLGEMPVPPYIKKKLKN
KDRYQTVYAKYEGSAAAPTAGLHFTEELLEKIRQMGVKTVFITLHVGLGTFRPVKEEVIENHKMHEEFYIVTKEAAEEIN
ETRKSGGRIIAVGTTSTRTLETVADEDGYIKEKSGWTDIFIYPGYKFKAIDGMITNFHLPESTLIMMVSAFAGKENIMRA
YKIAVEKKYRFFSFGDAMLII
>Mature_341_residues
MKRSEFYFELPEELIAQEPLEDRASSRLMILNKKTGEIEHDIFKNITKYLKPGDCLVLNNTKVIPARLIGRREDSGGKIE
FVLLKRISNNEWEILVKPGRRAKIGSKFVFGNGELIAEILDTTEVGGRIVRFYYEGVFEEVLDKLGEMPVPPYIKKKLKN
KDRYQTVYAKYEGSAAAPTAGLHFTEELLEKIRQMGVKTVFITLHVGLGTFRPVKEEVIENHKMHEEFYIVTKEAAEEIN
ETRKSGGRIIAVGTTSTRTLETVADEDGYIKEKSGWTDIFIYPGYKFKAIDGMITNFHLPESTLIMMVSAFAGKENIMRA
YKIAVEKKYRFFSFGDAMLII

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=46.8023255813954, Blast_Score=323, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38976; Mature: 38976

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSEFYFELPEELIAQEPLEDRASSRLMILNKKTGEIEHDIFKNITKYLKPGDCLVLNN
CCCCCEEEECCHHHHHCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECC
TKVIPARLIGRREDSGGKIEFVLLKRISNNEWEILVKPGRRAKIGSKFVFGNGELIAEIL
CEEEHHHHHCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCEEEECCCHHHHHHH
DTTEVGGRIVRFYYEGVFEEVLDKLGEMPVPPYIKKKLKNKDRYQTVYAKYEGSAAAPTA
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHEEEEEEECCCCCCCCC
GLHFTEELLEKIRQMGVKTVFITLHVGLGTFRPVKEEVIENHKMHEEFYIVTKEAAEEIN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHH
ETRKSGGRIIAVGTTSTRTLETVADEDGYIKEKSGWTDIFIYPGYKFKAIDGMITNFHLP
HHHHCCCEEEEEECCCCCCHHHHCCCCCEEEECCCCEEEEEECCEEEEEECCEEEEECCC
ESTLIMMVSAFAGKENIMRAYKIAVEKKYRFFSFGDAMLII
CHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEEECCEEEEC
>Mature Secondary Structure
MKRSEFYFELPEELIAQEPLEDRASSRLMILNKKTGEIEHDIFKNITKYLKPGDCLVLNN
CCCCCEEEECCHHHHHCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECC
TKVIPARLIGRREDSGGKIEFVLLKRISNNEWEILVKPGRRAKIGSKFVFGNGELIAEIL
CEEEHHHHHCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCEEEECCCHHHHHHH
DTTEVGGRIVRFYYEGVFEEVLDKLGEMPVPPYIKKKLKNKDRYQTVYAKYEGSAAAPTA
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHEEEEEEECCCCCCCCC
GLHFTEELLEKIRQMGVKTVFITLHVGLGTFRPVKEEVIENHKMHEEFYIVTKEAAEEIN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHH
ETRKSGGRIIAVGTTSTRTLETVADEDGYIKEKSGWTDIFIYPGYKFKAIDGMITNFHLP
HHHHCCCEEEEEECCCCCCHHHHCCCCCEEEECCCCEEEEEECCEEEEEECCEEEEECCC
ESTLIMMVSAFAGKENIMRAYKIAVEKKYRFFSFGDAMLII
CHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA