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The map label for this gene is cobS [H]

Identifier: 167038973

GI number: 167038973

Start: 324101

End: 324853

Strand: Direct

Name: cobS [H]

Synonym: Teth514_0306

Alternate gene names: 167038973

Gene position: 324101-324853 (Clockwise)

Preceding gene: 167038972

Following gene: 167038974

Centisome position: 13.19

GC content: 33.6

Gene sequence:

>753_bases
ATGGAAGAAATTAAAAGATTGGTACTTGCGATGCAGTTTATGACACACCTTCCTATACCAATAGAAATTGATGTTGAAAA
AAGTGAGTTTTATAAAATAGCATCTTATTTTCCAATAGTCGGAATTGTTATAGGAGGTATCCTTTCACTTTTTTATATTG
CTTTAAAGGATTTCTTTTCCCGGGAAATTGTAATGACTTTTATTGTAGCTTTTTCTTATGTTTTAACAGGAGCAATGCAT
ATTGACGGGCTGGCGGATACTTTTGATGGCCTTTTTAGCAATAAGGATAAAAGTAAAATGCTGGAGATAATGAGAGACTC
AAGGCTTGGCACAAATGGAGTCTTAGCTTTAGTTTTTATGGTGGTTTTAAAAATATTATTTCTCACCAATATAAACGAAA
ATATTACTTTAACAGCACTTTTAATTACCCCAATAATTGGGAGATTGTCAATTGTATTTTCTATGATGATAACTAAATCC
GCAAGAGGTGGAGAAGGCCTTGGAGGACTTATGCTGGGTAAAGTAGGGATAAGGGAGTTTGCTATAGCTTTTGTTATTTC
AATTGCTACAAGCTATTTTATACTGCCTTTAGCGGTTTTTGTAAAAATACTTACTATATCGCTTTTTGTTACTTATATTG
TATCAAAGTATATTTCGCTGAGAATAGGTGGTATGACTGGGGATACTCTCGGTGCAGTAAATGAATTGGCAGAGCTTACT
GCACTCATTTGCTTTGTAAGTGTGTCATTATAA

Upstream 100 bases:

>100_bases
ATGAATAAAAAAGTGGCAGATGCTGCAGATGAAGTTTATTTAATGGTTTCAGGAATACCTCTTAAAATAAAGGGATGATT
GAAAGGGATGCAATATCTTT

Downstream 100 bases:

>100_bases
AAAAGGAGAGATTTTTATGGGAAAAGGATTATTAATAATAGCACATGGAAGTCGTGTTGAAGAAACGAGGGAAGCAGTAT
ACCAAGTTATAAATAAAATA

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MEEIKRLVLAMQFMTHLPIPIEIDVEKSEFYKIASYFPIVGIVIGGILSLFYIALKDFFSREIVMTFIVAFSYVLTGAMH
IDGLADTFDGLFSNKDKSKMLEIMRDSRLGTNGVLALVFMVVLKILFLTNINENITLTALLITPIIGRLSIVFSMMITKS
ARGGEGLGGLMLGKVGIREFAIAFVISIATSYFILPLAVFVKILTISLFVTYIVSKYISLRIGGMTGDTLGAVNELAELT
ALICFVSVSL

Sequences:

>Translated_250_residues
MEEIKRLVLAMQFMTHLPIPIEIDVEKSEFYKIASYFPIVGIVIGGILSLFYIALKDFFSREIVMTFIVAFSYVLTGAMH
IDGLADTFDGLFSNKDKSKMLEIMRDSRLGTNGVLALVFMVVLKILFLTNINENITLTALLITPIIGRLSIVFSMMITKS
ARGGEGLGGLMLGKVGIREFAIAFVISIATSYFILPLAVFVKILTISLFVTYIVSKYISLRIGGMTGDTLGAVNELAELT
ALICFVSVSL
>Mature_250_residues
MEEIKRLVLAMQFMTHLPIPIEIDVEKSEFYKIASYFPIVGIVIGGILSLFYIALKDFFSREIVMTFIVAFSYVLTGAMH
IDGLADTFDGLFSNKDKSKMLEIMRDSRLGTNGVLALVFMVVLKILFLTNINENITLTALLITPIIGRLSIVFSMMITKS
ARGGEGLGGLMLGKVGIREFAIAFVISIATSYFILPLAVFVKILTISLFVTYIVSKYISLRIGGMTGDTLGAVNELAELT
ALICFVSVSL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=34.5528455284553, Blast_Score=134, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27479; Mature: 27479

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIKRLVLAMQFMTHLPIPIEIDVEKSEFYKIASYFPIVGIVIGGILSLFYIALKDFFS
CHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REIVMTFIVAFSYVLTGAMHIDGLADTFDGLFSNKDKSKMLEIMRDSRLGTNGVLALVFM
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHH
VVLKILFLTNINENITLTALLITPIIGRLSIVFSMMITKSARGGEGLGGLMLGKVGIREF
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AIAFVISIATSYFILPLAVFVKILTISLFVTYIVSKYISLRIGGMTGDTLGAVNELAELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALICFVSVSL
HHHHHHHHCC
>Mature Secondary Structure
MEEIKRLVLAMQFMTHLPIPIEIDVEKSEFYKIASYFPIVGIVIGGILSLFYIALKDFFS
CHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REIVMTFIVAFSYVLTGAMHIDGLADTFDGLFSNKDKSKMLEIMRDSRLGTNGVLALVFM
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHH
VVLKILFLTNINENITLTALLITPIIGRLSIVFSMMITKSARGGEGLGGLMLGKVGIREF
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AIAFVISIATSYFILPLAVFVKILTISLFVTYIVSKYISLRIGGMTGDTLGAVNELAELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALICFVSVSL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA