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The map label for this gene is lpdA [H]

Identifier: 167038903

GI number: 167038903

Start: 246037

End: 247692

Strand: Direct

Name: lpdA [H]

Synonym: Teth514_0234

Alternate gene names: 167038903

Gene position: 246037-247692 (Clockwise)

Preceding gene: 167038902

Following gene: 167038904

Centisome position: 10.01

GC content: 36.78

Gene sequence:

>1656_bases
ATGGAAATAGAAATAAAAATACCTGCTTTGTCAGATACGATGAAAAGTGGGCGAATTACAAAATGGTACGTAGAAGAAGG
ACAATACGTAGAAAAAGACTCTTGTCTATGTGATATAGCAGTAAATAAAGTAAATTTTGAAGTATACAGTGACTATGAAG
GCATTATTTCAAAAATTGTTTGTCCTGCTGGAAGTACAGTTGAACCGGGAGATGTTATTGCAATTGTTACACAGTCTGAA
GAAGTAAAGCCTTCTGCCACCCAAAAAGAAATTAAATATGAATACAAAGAATATGATTTAGCTGTCATAGGGGCTGGACC
AGGGGGATATGTGGCTGCTATTAAAGCTGCTAAAAAAGGTGCAAAAGTGGCTTTGTTTGAGAAGGATAAATTAGGTGGCA
CCTGTTTAAACAGAGGATGCATACCAACAAAAGCTTATGCGAGAATTGCTGAAGTGTATGACATTTTAAAACGGTCAGGA
GAATTTGGATTTGATGTGAAAGTAAATTCTTTTGATTACGCTAAAATTGTAAAAAGAAAAAATGACATTGTAGGAGAGCT
GACAGAAGGTATAAATGCTTTACTTAAAGCCAATGGAGTGGATTTGTTTTACGCTGAAGCAAAAGTTGACAAGGAAAAGA
ATGTGCTCTTTGGAGAAAACAAAATTAAAGCGAAAAATATTATCATTGCAACGGGGTCATCACCTGCTGAACTTCCTATC
GAAGGCATTAACTCCAAAAATGTCATGAATAGCGATGTTATTCTTGAAATTACCTCTTTGCCTCAAAGCTTATGTATTAT
AGGTGGCGGAGTAATAGGCATGGAATTCGCCTTTATAATGAACCAATTTGGAGTGAAAGTGTCTGTTGTTGAGATGATGC
CTGACATTCTGCCCATCCTTGATAAAGAAGTAAGTTCTTTTATTAGGGCAATAGCTCAAAAAAGAGGAATCAAGATATAT
ACTTCTTCAACGGTTGAGAGAATAGATGAAGAAGAAAATGGCGGAAGTATAGTAACAGTTAAAAATGGTGAAAATATAAA
ACGCATATACGCAGATAAAGTGTTTGTGTCTATAGGGAGGAAGTTAAATACAGATATTGGTCCAATTGTAGAACTATTAG
ATTTTGAAGGGAAAGCTATTAAAGTTGATGAGTACATGAGGACAAATGTAGAAGGGGTTTATGCAGTAGGTGATGTTACA
GGCAAAATGATGTTAGCCCATGTGGCTTCTGCACAAGGGGAGGTAGCAGTTGACAACATTTTTGGTGAATCAAATACATT
AGACTATATGAAAATACCTGCAGCAGTATTTACAGAACCAGAGATTGGGTATTTTGGCTATACCGAAGAAGAGGCGAGAA
AGAAATTTAAAGATATAAAAGTGGGAAGATTTAATTTTGAACACAATGGGAGAGCAAAGACATATGGAGAAACGGAAGGT
TTTGCAAAAGTAATTTCAAACGAAAAAGGAGAAGTAGTCGGTGTGTGGGTGGTAGGTAGTGGAGCTTCTGAACTTATCCA
CATACTTTCAACAGCCTGCCAAGAAGGTGTGGATGCAGAAGCTTTGAAGAAAGCGGTTTATGCCCATCCTACCCGAAGCG
AAACTATTATGGAAGCTGTTAAGGATATTTTCGGGGAGTCTGTACACAAAGTATAA

Upstream 100 bases:

>100_bases
TTATTTTATCAAATTTAATCCCCTCCCTAATGGGGAGCTATTACAAGAGATACGATTTGTAAGTGATTGATGAAACAGAA
AGTATTGGAGGTATATATAG

Downstream 100 bases:

>100_bases
TTTTAACGTTATTTATCTATAGTGCTTTCGAAGCTCTTAAAGGGCTTTCGAAATACATAAACTTTGAAAAATAAAAGGAG
GAGTTTAAAATGGCAATTGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MEIEIKIPALSDTMKSGRITKWYVEEGQYVEKDSCLCDIAVNKVNFEVYSDYEGIISKIVCPAGSTVEPGDVIAIVTQSE
EVKPSATQKEIKYEYKEYDLAVIGAGPGGYVAAIKAAKKGAKVALFEKDKLGGTCLNRGCIPTKAYARIAEVYDILKRSG
EFGFDVKVNSFDYAKIVKRKNDIVGELTEGINALLKANGVDLFYAEAKVDKEKNVLFGENKIKAKNIIIATGSSPAELPI
EGINSKNVMNSDVILEITSLPQSLCIIGGGVIGMEFAFIMNQFGVKVSVVEMMPDILPILDKEVSSFIRAIAQKRGIKIY
TSSTVERIDEEENGGSIVTVKNGENIKRIYADKVFVSIGRKLNTDIGPIVELLDFEGKAIKVDEYMRTNVEGVYAVGDVT
GKMMLAHVASAQGEVAVDNIFGESNTLDYMKIPAAVFTEPEIGYFGYTEEEARKKFKDIKVGRFNFEHNGRAKTYGETEG
FAKVISNEKGEVVGVWVVGSGASELIHILSTACQEGVDAEALKKAVYAHPTRSETIMEAVKDIFGESVHKV

Sequences:

>Translated_551_residues
MEIEIKIPALSDTMKSGRITKWYVEEGQYVEKDSCLCDIAVNKVNFEVYSDYEGIISKIVCPAGSTVEPGDVIAIVTQSE
EVKPSATQKEIKYEYKEYDLAVIGAGPGGYVAAIKAAKKGAKVALFEKDKLGGTCLNRGCIPTKAYARIAEVYDILKRSG
EFGFDVKVNSFDYAKIVKRKNDIVGELTEGINALLKANGVDLFYAEAKVDKEKNVLFGENKIKAKNIIIATGSSPAELPI
EGINSKNVMNSDVILEITSLPQSLCIIGGGVIGMEFAFIMNQFGVKVSVVEMMPDILPILDKEVSSFIRAIAQKRGIKIY
TSSTVERIDEEENGGSIVTVKNGENIKRIYADKVFVSIGRKLNTDIGPIVELLDFEGKAIKVDEYMRTNVEGVYAVGDVT
GKMMLAHVASAQGEVAVDNIFGESNTLDYMKIPAAVFTEPEIGYFGYTEEEARKKFKDIKVGRFNFEHNGRAKTYGETEG
FAKVISNEKGEVVGVWVVGSGASELIHILSTACQEGVDAEALKKAVYAHPTRSETIMEAVKDIFGESVHKV
>Mature_551_residues
MEIEIKIPALSDTMKSGRITKWYVEEGQYVEKDSCLCDIAVNKVNFEVYSDYEGIISKIVCPAGSTVEPGDVIAIVTQSE
EVKPSATQKEIKYEYKEYDLAVIGAGPGGYVAAIKAAKKGAKVALFEKDKLGGTCLNRGCIPTKAYARIAEVYDILKRSG
EFGFDVKVNSFDYAKIVKRKNDIVGELTEGINALLKANGVDLFYAEAKVDKEKNVLFGENKIKAKNIIIATGSSPAELPI
EGINSKNVMNSDVILEITSLPQSLCIIGGGVIGMEFAFIMNQFGVKVSVVEMMPDILPILDKEVSSFIRAIAQKRGIKIY
TSSTVERIDEEENGGSIVTVKNGENIKRIYADKVFVSIGRKLNTDIGPIVELLDFEGKAIKVDEYMRTNVEGVYAVGDVT
GKMMLAHVASAQGEVAVDNIFGESNTLDYMKIPAAVFTEPEIGYFGYTEEEARKKFKDIKVGRFNFEHNGRAKTYGETEG
FAKVISNEKGEVVGVWVVGSGASELIHILSTACQEGVDAEALKKAVYAHPTRSETIMEAVKDIFGESVHKV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=33.4754797441365, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI50301238, Length=458, Percent_Identity=31.0043668122271, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI291045266, Length=437, Percent_Identity=27.0022883295195, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI33519430, Length=455, Percent_Identity=26.3736263736264, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519428, Length=455, Percent_Identity=26.3736263736264, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519426, Length=455, Percent_Identity=26.3736263736264, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI148277065, Length=455, Percent_Identity=26.3736263736264, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI148277071, Length=455, Percent_Identity=26.3736263736264, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI22035672, Length=479, Percent_Identity=26.5135699373695, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI291045268, Length=433, Percent_Identity=25.4041570438799, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1786307, Length=449, Percent_Identity=32.739420935412, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=28.2110091743119, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI87082354, Length=452, Percent_Identity=28.7610619469027, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI87081717, Length=466, Percent_Identity=28.3261802575107, Blast_Score=165, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=34.7732181425486, Blast_Score=237, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI71983419, Length=467, Percent_Identity=27.8372591006424, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17557007, Length=483, Percent_Identity=29.399585921325, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=457, Percent_Identity=28.0087527352298, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71982272, Length=480, Percent_Identity=27.0833333333333, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=483, Percent_Identity=33.5403726708075, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=28.9583333333333, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=27.6231263383298, Blast_Score=149, Evalue=9e-37,
Organism=Drosophila melanogaster, GI21358499, Length=481, Percent_Identity=35.1351351351351, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24640553, Length=480, Percent_Identity=27.9166666666667, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640549, Length=480, Percent_Identity=27.9166666666667, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640551, Length=480, Percent_Identity=27.9166666666667, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17737741, Length=477, Percent_Identity=23.8993710691824, Blast_Score=115, Evalue=1e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 60179; Mature: 60179

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIEIKIPALSDTMKSGRITKWYVEEGQYVEKDSCLCDIAVNKVNFEVYSDYEGIISKIV
CEEEEECCCHHHHHHCCCEEEEEECCCCEECCCCCEEEEEEEEEEEEEECCHHHHHHHHC
CPAGSTVEPGDVIAIVTQSEEVKPSATQKEIKYEYKEYDLAVIGAGPGGYVAAIKAAKKG
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHEEEEEEEEEEEECCCCCCEEEEHHHCCC
AKVALFEKDKLGGTCLNRGCIPTKAYARIAEVYDILKRSGEFGFDVKVNSFDYAKIVKRK
CEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
NDIVGELTEGINALLKANGVDLFYAEAKVDKEKNVLFGENKIKAKNIIIATGSSPAELPI
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCEEEEEEEEECCCCCCCCCC
EGINSKNVMNSDVILEITSLPQSLCIIGGGVIGMEFAFIMNQFGVKVSVVEMMPDILPIL
CCCCCCCCCCCCEEEEEECCCCEEHHCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
DKEVSSFIRAIAQKRGIKIYTSSTVERIDEEENGGSIVTVKNGENIKRIYADKVFVSIGR
HHHHHHHHHHHHHHCCEEEEECHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
KLNTDIGPIVELLDFEGKAIKVDEYMRTNVEGVYAVGDVTGKMMLAHVASAQGEVAVDNI
CCCCCHHHHHHHHCCCCCEEEEHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEE
FGESNTLDYMKIPAAVFTEPEIGYFGYTEEEARKKFKDIKVGRFNFEHNGRAKTYGETEG
CCCCCCCCEEECCHHHCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCHH
FAKVISNEKGEVVGVWVVGSGASELIHILSTACQEGVDAEALKKAVYAHPTRSETIMEAV
HHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHH
KDIFGESVHKV
HHHHHHHHCCC
>Mature Secondary Structure
MEIEIKIPALSDTMKSGRITKWYVEEGQYVEKDSCLCDIAVNKVNFEVYSDYEGIISKIV
CEEEEECCCHHHHHHCCCEEEEEECCCCEECCCCCEEEEEEEEEEEEEECCHHHHHHHHC
CPAGSTVEPGDVIAIVTQSEEVKPSATQKEIKYEYKEYDLAVIGAGPGGYVAAIKAAKKG
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHEEEEEEEEEEEECCCCCCEEEEHHHCCC
AKVALFEKDKLGGTCLNRGCIPTKAYARIAEVYDILKRSGEFGFDVKVNSFDYAKIVKRK
CEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
NDIVGELTEGINALLKANGVDLFYAEAKVDKEKNVLFGENKIKAKNIIIATGSSPAELPI
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCEEEEEEEEECCCCCCCCCC
EGINSKNVMNSDVILEITSLPQSLCIIGGGVIGMEFAFIMNQFGVKVSVVEMMPDILPIL
CCCCCCCCCCCCEEEEEECCCCEEHHCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
DKEVSSFIRAIAQKRGIKIYTSSTVERIDEEENGGSIVTVKNGENIKRIYADKVFVSIGR
HHHHHHHHHHHHHHCCEEEEECHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
KLNTDIGPIVELLDFEGKAIKVDEYMRTNVEGVYAVGDVTGKMMLAHVASAQGEVAVDNI
CCCCCHHHHHHHHCCCCCEEEEHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEE
FGESNTLDYMKIPAAVFTEPEIGYFGYTEEEARKKFKDIKVGRFNFEHNGRAKTYGETEG
CCCCCCCCEEECCHHHCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCHH
FAKVISNEKGEVVGVWVVGSGASELIHILSTACQEGVDAEALKKAVYAHPTRSETIMEAV
HHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHH
KDIFGESVHKV
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]