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The map label for this gene is lplA [H]

Identifier: 167038902

GI number: 167038902

Start: 244872

End: 245852

Strand: Direct

Name: lplA [H]

Synonym: Teth514_0233

Alternate gene names: 167038902

Gene position: 244872-245852 (Clockwise)

Preceding gene: 167038897

Following gene: 167038903

Centisome position: 9.97

GC content: 30.99

Gene sequence:

>981_bases
ATGTTATATATTTACAATAAGAACACAAATCCTTATTTTAATTTAGCTGCTGAAGAGTACGTTTTAAAAGAATTCCAAGA
AGAATGTTTCATGTTATGGAGAAATGAACCTAGCATTATAGTAGGCAAAAATCAAAACACATTAGCGGAAATAAATTTGG
ATTATGTGCGACAACATAAAATTCCAGTTGTCAGAAGATTATCGGGTGGAGGAGCGGTTTTTCACGACCTCGGCAATTTA
AATTTTACTTTTATAGTAAATGAGGATGTCAGCAGTTTCAGTGATTTTAAAAGATTTACACAGCCAATTATAGATGTGCT
TAGAAAACTTTCTGTTAATGCTGAGTTTTCAGGGAGAAATGACATAACAATTGACGGCAAAAAAATTTCAGGAAATGCGC
AGTATTATTACAAAAATAGGATATTACACCATGGAACACTTTTGTTTTCTTCCAGCATTACAGATTTGTCAGCAGCACTG
AAAGTGCGACCTGTAAAATTTGAAGATAAAGGTGTAAAATCTGTGTCTAAAAGAGTTACAAATATAAGTGAGCATCTAAA
AGAGCCTATTACAATAGAACAGTTTATAGACCTCATTATGAATCACATTAGAGAACAAACCGGTGGAAGGGAAATGTATG
AATTTACGCAGGAAGACATAAAAAAAATAGAGAAATTGGTTAAAGAAAAGTATAGCACATGGGAATGGAATTTTGGAACA
TCGCCAGATTACAGTTTTAAAAATGAGAAAAAATTTACTGGTGGCACTGTAGAAGTAAATTTAAATGTAGAAAAGGGAAT
AATAAAAGATATAAAAATTTATGGAGACTTTTTTGGAAAATACGATGTTTCAGAGATAGAAAACCTTTTGAAAGGAGTAA
AGCATTCAGAAGAGGAAATAAAAAAAGTACTTTCAAATATTGATATAAACGATTATTTTGCCAACATAACTGTTGACAAT
TTAATAGAAGTGATGTTTTGA

Upstream 100 bases:

>100_bases
TCTTCATCTAGAACAGTATTAAATTTTTTTAAAATATGCTATACTTTTATTATATTATATTCCCTGTAATAGTCAATAAT
CAAAACAAGGAGAGGTGGAT

Downstream 100 bases:

>100_bases
TAAAAAAATATTTTTAAATTGTTGAATTTTTAACTAAAAAGTGATAAGATATATATGAAGGAAATTGTTAAATAATTGGC
GTTTTTATTTTATCAAATTT

Product: lipoyltransferase and lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MLYIYNKNTNPYFNLAAEEYVLKEFQEECFMLWRNEPSIIVGKNQNTLAEINLDYVRQHKIPVVRRLSGGGAVFHDLGNL
NFTFIVNEDVSSFSDFKRFTQPIIDVLRKLSVNAEFSGRNDITIDGKKISGNAQYYYKNRILHHGTLLFSSSITDLSAAL
KVRPVKFEDKGVKSVSKRVTNISEHLKEPITIEQFIDLIMNHIREQTGGREMYEFTQEDIKKIEKLVKEKYSTWEWNFGT
SPDYSFKNEKKFTGGTVEVNLNVEKGIIKDIKIYGDFFGKYDVSEIENLLKGVKHSEEEIKKVLSNIDINDYFANITVDN
LIEVMF

Sequences:

>Translated_326_residues
MLYIYNKNTNPYFNLAAEEYVLKEFQEECFMLWRNEPSIIVGKNQNTLAEINLDYVRQHKIPVVRRLSGGGAVFHDLGNL
NFTFIVNEDVSSFSDFKRFTQPIIDVLRKLSVNAEFSGRNDITIDGKKISGNAQYYYKNRILHHGTLLFSSSITDLSAAL
KVRPVKFEDKGVKSVSKRVTNISEHLKEPITIEQFIDLIMNHIREQTGGREMYEFTQEDIKKIEKLVKEKYSTWEWNFGT
SPDYSFKNEKKFTGGTVEVNLNVEKGIIKDIKIYGDFFGKYDVSEIENLLKGVKHSEEEIKKVLSNIDINDYFANITVDN
LIEVMF
>Mature_326_residues
MLYIYNKNTNPYFNLAAEEYVLKEFQEECFMLWRNEPSIIVGKNQNTLAEINLDYVRQHKIPVVRRLSGGGAVFHDLGNL
NFTFIVNEDVSSFSDFKRFTQPIIDVLRKLSVNAEFSGRNDITIDGKKISGNAQYYYKNRILHHGTLLFSSSITDLSAAL
KVRPVKFEDKGVKSVSKRVTNISEHLKEPITIEQFIDLIMNHIREQTGGREMYEFTQEDIKKIEKLVKEKYSTWEWNFGT
SPDYSFKNEKKFTGGTVEVNLNVEKGIIKDIKIYGDFFGKYDVSEIENLLKGVKHSEEEIKKVLSNIDINDYFANITVDN
LIEVMF

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=251, Percent_Identity=31.8725099601594, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI21729884, Length=251, Percent_Identity=31.8725099601594, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI21729882, Length=251, Percent_Identity=31.8725099601594, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI21729880, Length=251, Percent_Identity=31.8725099601594, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI21729878, Length=251, Percent_Identity=31.8725099601594, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1790846, Length=303, Percent_Identity=33.993399339934, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71981909, Length=256, Percent_Identity=28.515625, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6322415, Length=281, Percent_Identity=30.6049822064057, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28573464, Length=332, Percent_Identity=26.2048192771084, Blast_Score=124, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37779; Mature: 37779

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYIYNKNTNPYFNLAAEEYVLKEFQEECFMLWRNEPSIIVGKNQNTLAEINLDYVRQHK
CEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEHHHHHHCC
IPVVRRLSGGGAVFHDLGNLNFTFIVNEDVSSFSDFKRFTQPIIDVLRKLSVNAEFSGRN
CCCEEEECCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
DITIDGKKISGNAQYYYKNRILHHGTLLFSSSITDLSAALKVRPVKFEDKGVKSVSKRVT
CEEEECEEECCCEEEEEECCEEECCEEEEECCHHHHHHHHEEECEEECCCHHHHHHHHHH
NISEHLKEPITIEQFIDLIMNHIREQTGGREMYEFTQEDIKKIEKLVKEKYSTWEWNFGT
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCC
SPDYSFKNEKKFTGGTVEVNLNVEKGIIKDIKIYGDFFGKYDVSEIENLLKGVKHSEEEI
CCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHH
KKVLSNIDINDYFANITVDNLIEVMF
HHHHHHCCCCHHEECCCHHHHHHHCC
>Mature Secondary Structure
MLYIYNKNTNPYFNLAAEEYVLKEFQEECFMLWRNEPSIIVGKNQNTLAEINLDYVRQHK
CEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEHHHHHHCC
IPVVRRLSGGGAVFHDLGNLNFTFIVNEDVSSFSDFKRFTQPIIDVLRKLSVNAEFSGRN
CCCEEEECCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
DITIDGKKISGNAQYYYKNRILHHGTLLFSSSITDLSAALKVRPVKFEDKGVKSVSKRVT
CEEEECEEECCCEEEEEECCEEECCEEEEECCHHHHHHHHEEECEEECCCHHHHHHHHHH
NISEHLKEPITIEQFIDLIMNHIREQTGGREMYEFTQEDIKKIEKLVKEKYSTWEWNFGT
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCC
SPDYSFKNEKKFTGGTVEVNLNVEKGIIKDIKIYGDFFGKYDVSEIENLLKGVKHSEEEI
CCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHH
KKVLSNIDINDYFANITVDNLIEVMF
HHHHHHCCCCHHEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]