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Identifier: 16519944

GI number: 16519944

Start: 197282

End: 197893

Strand: Direct

Name: Not Available

Synonym: NGR_a01610

Alternate gene names: 16519944

Gene position: 197282-197893 (Clockwise)

Preceding gene: 16519947

Following gene: 16519943

Centisome position: 36.8

GC content: 63.24

Gene sequence:

>612_bases
TTGGGCGTCCGCTTCTCCTGGGGCACGGAAGTCAGGAAACTGGATGTCGAGGGCGACAAGGTCAGGGGTGTCGTAACCAA
CTGGGAAAGGCTCGCGGCCGATGCTGTTGTCGTCGCACTCGGCAGCTATTCGCCGCTACTGCTGAAGCGTCACGGCATCA
AGCTGCCGGTCTACCCGGTCAAAGGCTATTCGCTGACGATCCCGATCACCGATGCCTCGCGGGCGCCGGAATCGACCATC
ATGGACGAGACGTACAAGATCGCCATCACGCGCCTTGGCGACCGCATCCGCGTCGGCGGCATGGCAGAAATTTCCGGATA
CACCAATGATCTCGGCCCCGCCCGGCGACGCACACTCGAGCATTCCGTCATGGACCTCTTTCCCGGCGGCGATGCAAAAG
AAGCATCCTATTGGTCGGGTCTGCGCCCAATGACGCCGGACGGCACGCCCGTCATCGGCCCAACGAAGATCGCCGGTCTC
TTCCTTAACACCGGGCACGGCACGCTTGGCTGGACGATGAGCTCCGGCTCTGCCCGCGTCATTGCGGACCTGGTATCTGG
CCGGAAGCCCGAGATCGACGCCACCGACCTCGCCGTCAGCCGCTACGCTTGA

Upstream 100 bases:

>100_bases
ACCCGCGCGCGACAAGATCGTCGGTGGGCTGCTGACGCCGAAGGACGAGACCGGCGACTGCTTCAAGTTCACCAACGCGC
TTGCGAAGAAGGCGGAGCAA

Downstream 100 bases:

>100_bases
GCACTCACTGGATACCAATGGAGGACTAAAACTTTGATCGAACCCATTTCTACAAACGATGCCCCCGGCGCGGTCGGCCC
CTACTCCCAGGCCATCAAGG

Product: D-amino acid dehydrogenase

Products: 2-oxo acid; NH3; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MGVRFSWGTEVRKLDVEGDKVRGVVTNWERLAADAVVVALGSYSPLLLKRHGIKLPVYPVKGYSLTIPITDASRAPESTI
MDETYKIAITRLGDRIRVGGMAEISGYTNDLGPARRRTLEHSVMDLFPGGDAKEASYWSGLRPMTPDGTPVIGPTKIAGL
FLNTGHGTLGWTMSSGSARVIADLVSGRKPEIDATDLAVSRYA

Sequences:

>Translated_203_residues
MGVRFSWGTEVRKLDVEGDKVRGVVTNWERLAADAVVVALGSYSPLLLKRHGIKLPVYPVKGYSLTIPITDASRAPESTI
MDETYKIAITRLGDRIRVGGMAEISGYTNDLGPARRRTLEHSVMDLFPGGDAKEASYWSGLRPMTPDGTPVIGPTKIAGL
FLNTGHGTLGWTMSSGSARVIADLVSGRKPEIDATDLAVSRYA
>Mature_202_residues
GVRFSWGTEVRKLDVEGDKVRGVVTNWERLAADAVVVALGSYSPLLLKRHGIKLPVYPVKGYSLTIPITDASRAPESTIM
DETYKIAITRLGDRIRVGGMAEISGYTNDLGPARRRTLEHSVMDLFPGGDAKEASYWSGLRPMTPDGTPVIGPTKIAGLF
LNTGHGTLGWTMSSGSARVIADLVSGRKPEIDATDLAVSRYA

Specific function: Either a functional dehydrogenase or a non-functional fragment

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family

Homologues:

Organism=Escherichia coli, GI1787438, Length=204, Percent_Identity=57.3529411764706, Blast_Score=229, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y4SL_RHISN (P55655)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_444064.1
- ProteinModelPortal:   P55655
- GeneID:   962439
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01610
- HOGENOM:   HBG655890
- HAMAP:   MF_01202
- InterPro:   IPR006076

Pfam domain/function: PF01266 DAO

EC number: 1.4.99.1

Molecular weight: Translated: 21888; Mature: 21757

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVRFSWGTEVRKLDVEGDKVRGVVTNWERLAADAVVVALGSYSPLLLKRHGIKLPVYPV
CCCCCCCCCCEEEEECCCCEEEEEECCHHHHHHCEEEEEECCCCCEEEHHCCCEEEEEEE
KGYSLTIPITDASRAPESTIMDETYKIAITRLGDRIRVGGMAEISGYTNDLGPARRRTLE
CCCEEEEEECCCCCCCCCHHCCCCEEEEEEECCCEEEECCEEEECCCCCCCCHHHHHHHH
HSVMDLFPGGDAKEASYWSGLRPMTPDGTPVIGPTKIAGLFLNTGHGTLGWTMSSGSARV
HHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHH
IADLVSGRKPEIDATDLAVSRYA
HHHHHCCCCCCCCHHHEEEECCC
>Mature Secondary Structure 
GVRFSWGTEVRKLDVEGDKVRGVVTNWERLAADAVVVALGSYSPLLLKRHGIKLPVYPV
CCCCCCCCCEEEEECCCCEEEEEECCHHHHHHCEEEEEECCCCCEEEHHCCCEEEEEEE
KGYSLTIPITDASRAPESTIMDETYKIAITRLGDRIRVGGMAEISGYTNDLGPARRRTLE
CCCEEEEEECCCCCCCCCHHCCCCEEEEEEECCCEEEECCEEEECCCCCCCCHHHHHHHH
HSVMDLFPGGDAKEASYWSGLRPMTPDGTPVIGPTKIAGLFLNTGHGTLGWTMSSGSARV
HHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHH
IADLVSGRKPEIDATDLAVSRYA
HHHHHCCCCCCCCHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 19 {D-serine}} 12 {D-alpha-aminobutyrate}} 6 {D-phenylalanine}} 0.15 {cytochrome} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-amino acid; H2O; acceptor

Specific reaction: a D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424