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Identifier: 16519939

GI number: 16519939

Start: 207279

End: 208289

Strand: Reverse

Name: Not Available

Synonym: NGR_a01660

Alternate gene names: 16519939

Gene position: 208289-207279 (Counterclockwise)

Preceding gene: 16519933

Following gene: 16519940

Centisome position: 38.85

GC content: 57.86

Gene sequence:

>1011_bases
ATGCGAATAGCCGTCGTGTGTAACAGTGCTTTTACGGGTGTAATCAATCGGTTCGGTCAGCCTTATCCGCAGCCGCCGCA
GCCTTGGCCGCAGGGCCGGATAGCGGACAGCGTGGTGGCGGCCCTGCAAGAATGCGGCCACGAGACGCTGCTGTGTGAAG
GCGACAAAGGACTGCTCACTACGCTTGAGCGGTTCATGCCACCCGATCCGCTAGCCCGTACTTCGGGAATGGTCTTCAAC
TTGGCCGAGGGAATTCAGGGCGAGTACCGTTTCACCCATGTTCCGGCTATGCTCGAGATGGCCGGCGTCCCCTACACCGG
ATCGAGCCCTCTGGGGCATGGGCTGACCGACGATAAGGTCATCAGCAAGACGCTTATGCGCGATAGCGGCGTGCCCACGC
CGAACTTTAGTATTATGCGCCGCGGCACCGAGAGTACCGACGATCTCCGATTCCCAGTCGTGGTGAAACCGCGTCACGAG
GACAACAGCTTCGGATTGCAGCTCGTACATGAGCCGGCTCAATTGCAGCAAGCAGTGAAAATGATCGTCACGAAGTATGC
GCAGGATGCGCTTGTGGAAGAATACATCGATGGGCGAGAAATCCACGTCGCGCTGTTGGGAAACCAAGAAGTCGAGGTGT
TGCCTATGGTGGAATTTGAGCTTGGCGAACACGAAGCCCCTCTTCTAACCTGGGAAGCGAAGTACTTGGCGGCCGTGCAG
CCGCCAAAGACCTGTCCGGCGAAAATCGAGAGCAAGCTCGCGACTTTGCTGCGGGATATTTCTGTTGCGACCTTCCGCGC
CTGCCAGTGCCGGGATTATGCTCGCGTCGACCTCCGGCTCGACCGTTCCGGCCAGCCCTTTGTTCTTGAGATAAACTCGA
TGCCGGGACTCAGTACTCACTCCGCTTATGTTTTAGCCGCGATGACTGCCGGACACAGTTACTCTAGCTTGATCAATGGC
ATCCTCGACGTCGCCCATAGGCGGTACTTCGGAAACGGCATCCTAGGGTGA

Upstream 100 bases:

>100_bases
TCCGTGTAAGCGCGTGGGATGAGACCAACTGTTACCCTCGATTAGCGACCAAATTCGAGCAAATGAGAGTGACAGTTACC
AGACAACCAAGGAGTAGCTC

Downstream 100 bases:

>100_bases
GGGTGGTTCAGTCATCATAGCCCAAAGATGGCGGCAACTGCGATACAGATTTCCGAAAAAGGTTTAGGCGTATCGGTCGC
AGCAGCTGTCAATCTGATCC

Product: D-alanine--D-alanine ligase

Products: ADP; D-Alanyl-D-alanine; H+; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MRIAVVCNSAFTGVINRFGQPYPQPPQPWPQGRIADSVVAALQECGHETLLCEGDKGLLTTLERFMPPDPLARTSGMVFN
LAEGIQGEYRFTHVPAMLEMAGVPYTGSSPLGHGLTDDKVISKTLMRDSGVPTPNFSIMRRGTESTDDLRFPVVVKPRHE
DNSFGLQLVHEPAQLQQAVKMIVTKYAQDALVEEYIDGREIHVALLGNQEVEVLPMVEFELGEHEAPLLTWEAKYLAAVQ
PPKTCPAKIESKLATLLRDISVATFRACQCRDYARVDLRLDRSGQPFVLEINSMPGLSTHSAYVLAAMTAGHSYSSLING
ILDVAHRRYFGNGILG

Sequences:

>Translated_336_residues
MRIAVVCNSAFTGVINRFGQPYPQPPQPWPQGRIADSVVAALQECGHETLLCEGDKGLLTTLERFMPPDPLARTSGMVFN
LAEGIQGEYRFTHVPAMLEMAGVPYTGSSPLGHGLTDDKVISKTLMRDSGVPTPNFSIMRRGTESTDDLRFPVVVKPRHE
DNSFGLQLVHEPAQLQQAVKMIVTKYAQDALVEEYIDGREIHVALLGNQEVEVLPMVEFELGEHEAPLLTWEAKYLAAVQ
PPKTCPAKIESKLATLLRDISVATFRACQCRDYARVDLRLDRSGQPFVLEINSMPGLSTHSAYVLAAMTAGHSYSSLING
ILDVAHRRYFGNGILG
>Mature_336_residues
MRIAVVCNSAFTGVINRFGQPYPQPPQPWPQGRIADSVVAALQECGHETLLCEGDKGLLTTLERFMPPDPLARTSGMVFN
LAEGIQGEYRFTHVPAMLEMAGVPYTGSSPLGHGLTDDKVISKTLMRDSGVPTPNFSIMRRGTESTDDLRFPVVVKPRHE
DNSFGLQLVHEPAQLQQAVKMIVTKYAQDALVEEYIDGREIHVALLGNQEVEVLPMVEFELGEHEAPLLTWEAKYLAAVQ
PPKTCPAKIESKLATLLRDISVATFRACQCRDYARVDLRLDRSGQPFVLEINSMPGLSTHSAYVLAAMTAGHSYSSLING
ILDVAHRRYFGNGILG

Specific function: Could be involved in the biosynthesis of a cell wall component

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=240, Percent_Identity=31.6666666666667, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1786579, Length=269, Percent_Identity=27.5092936802974, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y4SG_RHISN (P55650)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_444059.1
- ProteinModelPortal:   P55650
- SMR:   P55650
- GeneID:   962220
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01660
- HOGENOM:   HBG427092
- ProtClustDB:   CLSK516124
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011095
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20

Pfam domain/function: PF07478 Dala_Dala_lig_C; SSF52440 PreATP-grasp-like

EC number: 6.3.2.4 [C]

Molecular weight: Translated: 36973; Mature: 36973

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAVVCNSAFTGVINRFGQPYPQPPQPWPQGRIADSVVAALQECGHETLLCEGDKGLLT
CEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHH
TLERFMPPDPLARTSGMVFNLAEGIQGEYRFTHVPAMLEMAGVPYTGSSPLGHGLTDDKV
HHHHHCCCCCCHHHCCCEEEHHHCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCHHH
ISKTLMRDSGVPTPNFSIMRRGTESTDDLRFPVVVKPRHEDNSFGLQLVHEPAQLQQAVK
HHHHHHHCCCCCCCCHHHEECCCCCCCCEECCEEEECCCCCCCCCEEEECCHHHHHHHHH
MIVTKYAQDALVEEYIDGREIHVALLGNQEVEVLPMVEFELGEHEAPLLTWEAKYLAAVQ
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEEEECCCCCCCEEEECHHEEEECC
PPKTCPAKIESKLATLLRDISVATFRACQCRDYARVDLRLDRSGQPFVLEINSMPGLSTH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCCCC
SAYVLAAMTAGHSYSSLINGILDVAHRRYFGNGILG
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRIAVVCNSAFTGVINRFGQPYPQPPQPWPQGRIADSVVAALQECGHETLLCEGDKGLLT
CEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHH
TLERFMPPDPLARTSGMVFNLAEGIQGEYRFTHVPAMLEMAGVPYTGSSPLGHGLTDDKV
HHHHHCCCCCCHHHCCCEEEHHHCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCHHH
ISKTLMRDSGVPTPNFSIMRRGTESTDDLRFPVVVKPRHEDNSFGLQLVHEPAQLQQAVK
HHHHHHHCCCCCCCCHHHEECCCCCCCCEECCEEEECCCCCCCCCEEEECCHHHHHHHHH
MIVTKYAQDALVEEYIDGREIHVALLGNQEVEVLPMVEFELGEHEAPLLTWEAKYLAAVQ
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEEEECCCCCCCEEEECHHEEEECC
PPKTCPAKIESKLATLLRDISVATFRACQCRDYARVDLRLDRSGQPFVLEINSMPGLSTH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCCCC
SAYVLAAMTAGHSYSSLINGILDVAHRRYFGNGILG
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1018 [C]

Specific activity: 31

Km value (mM): 15 {D-norleucine}} 6 {D-Phe}} 40 {ATP}} 2.5 {ATP}} 2.4 {ATP}} 0.179 {ATP}} 0.161 {ATP}} 0.055 {ATP}} 0.049 {ATP}} 0.033 {ATP}} 0.008 {ATP}} 9 {D-Met}} 86 {D-Ala}} 70.3 {D-Ala}} 60.4 {D-Ala}} 57.5 {D-

Substrates: D-Alanine; ATP [C]

Specific reaction: (2) D-Alanine + ATP <==> ADP + D-Alanyl-D-alanine + H+ + Phosphate [C]

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: [1 (S)-Aminoethyl] [(2RS) 2-carboxy-1-octyl] phosphinic acid; Aminoalkyl phosphinate; D-Ala-D-2-hydroxy; D-Ala-D-Ala; D-Ala-D-Alaphosphinate; D-Cycloserine; Gly; Gly-Gly; Phosphinates; Phosphonate dipeptide analogs [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424