BacMap

Definition	Sinorhizobium fredii NGR234 plasmid pNGR234a, complete sequence.
Accession	NC_000914
Length	536,165

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The map label for this gene is Not Available

Identifier: 16519722

GI number: 16519722

Start: 493676

End: 494422

Strand: Reverse

Name: Not Available

Synonym: NGR_a03840

Alternate gene names: 16519722

Gene position: 494422-493676 (Counterclockwise)

Preceding gene: 16519718

Following gene: 16519723

Centisome position: 92.21

GC content: 58.9

Gene sequence:

>747_bases
TTGCATCCGATGAACAGATACGAAGTTGCCCTTGTCACAGGGGCCTCGAGCGGTATTGGGAAGGCAATCGCGCTTGAGCT
TGCCTCGGCCGGGCTCAGGGTCCTTGCTCTTGGCAGGGACCGTGCTGCGCTTGACGAGCTTCACTCGACCGCGGGCATCG
TGCCCGTCGTGTTCGATCTCTCTGACGTTTCCGAAGTCTATGGGAAAATCGCGGGGGAAAAGATCGACGTGCTGGTGAAC
AACGCCGGATTACTCACCGCCTCGGCAAGCCTCGTCGACCTGTCTGAAGACGATATTGACGCAATGATCGACATCAACAT
TCGGTCGGTCTTCAAGCTGACGCGGCATGTCCTGAAGCAGATGATCGAACGTCGCCGCGGCCATATCTTTTTCACCGGTT
CAAGCGGCGGCCTTGCCCCTCATCCCAACTCTTCCGTTTACGGCGCGACGAAGGCGGCCGTCAGCCTGTTTTCATCTGCA
CTGCGCTGCGATCTGATCGGTCTACCGATCAGGGTGACGGAGCTTTTTCCCGGCCGCGTTGAGACGAACCTCTATCGGAC
AGCGCTCGGCAAAGAGGGCGCAAAGAAGAAGCTCTACGACGACAATGAGGCGATCCAGCCCGAACACATGGCGCGACTGC
TGCGTACGGCGCTGGAGTTGCCGGACTTCGTCGACGTAACCCGCCTGGAAGTGATGCCGACGGGCCAAGTAGTCGGCGGC
GCCCAGATGTCCAAACTTTCTCGTTAA

Upstream 100 bases:

>100_bases
GCCTGTCGATTGCCGCTTCGGACGCGGAACTCGAAAGCGCGTGTGAACGTCTCGCCAAGCTGGTTGCGTCGCTTAGCAAA
TAGCACTCGAGGACTAAGGA

Downstream 100 bases:

>100_bases
CTGCGAACCTTGGAAGGAAAAGATCATGCAGGGTATGAACATCGCCGTCGTCGGTGGTGCGAGCGGCTTCGGCCTCGAAA
CGGCAAAGCTCATGGTCCGT

Product: short chain dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MHPMNRYEVALVTGASSGIGKAIALELASAGLRVLALGRDRAALDELHSTAGIVPVVFDLSDVSEVYGKIAGEKIDVLVN
NAGLLTASASLVDLSEDDIDAMIDINIRSVFKLTRHVLKQMIERRRGHIFFTGSSGGLAPHPNSSVYGATKAAVSLFSSA
LRCDLIGLPIRVTELFPGRVETNLYRTALGKEGAKKKLYDDNEAIQPEHMARLLRTALELPDFVDVTRLEVMPTGQVVGG
AQMSKLSR

Sequences:

>Translated_248_residues
MHPMNRYEVALVTGASSGIGKAIALELASAGLRVLALGRDRAALDELHSTAGIVPVVFDLSDVSEVYGKIAGEKIDVLVN
NAGLLTASASLVDLSEDDIDAMIDINIRSVFKLTRHVLKQMIERRRGHIFFTGSSGGLAPHPNSSVYGATKAAVSLFSSA
LRCDLIGLPIRVTELFPGRVETNLYRTALGKEGAKKKLYDDNEAIQPEHMARLLRTALELPDFVDVTRLEVMPTGQVVGG
AQMSKLSR
>Mature_248_residues
MHPMNRYEVALVTGASSGIGKAIALELASAGLRVLALGRDRAALDELHSTAGIVPVVFDLSDVSEVYGKIAGEKIDVLVN
NAGLLTASASLVDLSEDDIDAMIDINIRSVFKLTRHVLKQMIERRRGHIFFTGSSGGLAPHPNSSVYGATKAAVSLFSSA
LRCDLIGLPIRVTELFPGRVETNLYRTALGKEGAKKKLYDDNEAIQPEHMARLLRTALELPDFVDVTRLEVMPTGQVVGG
AQMSKLSR

Specific function: Unknown

COG id: COG4221

COG function: function code R; Short-chain alcohol dehydrogenase of unknown specificity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family

Homologues:

Organism=Homo sapiens, GI7705855, Length=190, Percent_Identity=35.2631578947368, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI20149619, Length=215, Percent_Identity=32.093023255814, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4557649, Length=185, Percent_Identity=36.7567567567568, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI109715829, Length=256, Percent_Identity=30.859375, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI226371731, Length=186, Percent_Identity=32.258064516129, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI7706318, Length=202, Percent_Identity=32.6732673267327, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI32483357, Length=219, Percent_Identity=30.1369863013699, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI209862901, Length=207, Percent_Identity=32.3671497584541, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI19923983, Length=207, Percent_Identity=32.3671497584541, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI7705925, Length=178, Percent_Identity=32.5842696629214, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI66933014, Length=161, Percent_Identity=29.8136645962733, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4503817, Length=218, Percent_Identity=27.9816513761468, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI223718074, Length=245, Percent_Identity=28.1632653061224, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI5031737, Length=205, Percent_Identity=30.2439024390244, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI4504503, Length=204, Percent_Identity=27.9411764705882, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI22507405, Length=201, Percent_Identity=27.8606965174129, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI7657478, Length=186, Percent_Identity=32.258064516129, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI126723191, Length=179, Percent_Identity=30.7262569832402, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787820, Length=246, Percent_Identity=34.9593495934959, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87082100, Length=218, Percent_Identity=34.8623853211009, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787335, Length=186, Percent_Identity=34.4086021505376, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786812, Length=189, Percent_Identity=31.7460317460317, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87082160, Length=192, Percent_Identity=29.6875, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786701, Length=181, Percent_Identity=32.5966850828729, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788459, Length=192, Percent_Identity=29.1666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787526, Length=201, Percent_Identity=28.3582089552239, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367175, Length=192, Percent_Identity=29.6875, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=195, Percent_Identity=35.3846153846154, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115534694, Length=198, Percent_Identity=34.8484848484849, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560676, Length=200, Percent_Identity=28, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17538486, Length=243, Percent_Identity=30.0411522633745, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193204405, Length=248, Percent_Identity=30.241935483871, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17562910, Length=213, Percent_Identity=31.4553990610329, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559104, Length=217, Percent_Identity=30.8755760368664, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17561402, Length=222, Percent_Identity=29.7297297297297, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=213, Percent_Identity=29.5774647887324, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560332, Length=228, Percent_Identity=31.140350877193, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994600, Length=196, Percent_Identity=30.1020408163265, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17557668, Length=190, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=240, Percent_Identity=28.75, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=242, Percent_Identity=29.7520661157025, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=237, Percent_Identity=29.957805907173, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560176, Length=193, Percent_Identity=27.4611398963731, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25152911, Length=192, Percent_Identity=29.6875, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71997402, Length=223, Percent_Identity=28.6995515695067, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17562990, Length=229, Percent_Identity=29.6943231441048, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=186, Percent_Identity=28.494623655914, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17562908, Length=196, Percent_Identity=32.1428571428571, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=216, Percent_Identity=28.2407407407407, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=202, Percent_Identity=31.1881188118812, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI212646214, Length=190, Percent_Identity=27.3684210526316, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=241, Percent_Identity=26.1410788381743, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=204, Percent_Identity=28.921568627451, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17565030, Length=237, Percent_Identity=29.535864978903, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17555706, Length=249, Percent_Identity=28.9156626506024, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=188, Percent_Identity=27.1276595744681, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=204, Percent_Identity=27.9411764705882, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=248, Percent_Identity=31.4516129032258, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322067, Length=245, Percent_Identity=28.1632653061224, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24584748, Length=185, Percent_Identity=38.3783783783784, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI161076933, Length=185, Percent_Identity=38.3783783783784, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI28574402, Length=188, Percent_Identity=37.2340425531915, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24584744, Length=188, Percent_Identity=37.2340425531915, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI281364494, Length=257, Percent_Identity=29.9610894941634, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI23397609, Length=186, Percent_Identity=33.8709677419355, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI116007236, Length=256, Percent_Identity=31.640625, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357041, Length=198, Percent_Identity=33.8383838383838, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24641232, Length=258, Percent_Identity=31.0077519379845, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640442, Length=193, Percent_Identity=35.7512953367876, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28571526, Length=191, Percent_Identity=31.9371727748691, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21355631, Length=169, Percent_Identity=31.9526627218935, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648944, Length=237, Percent_Identity=32.0675105485232, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24580925, Length=265, Percent_Identity=28.3018867924528, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21358495, Length=236, Percent_Identity=28.8135593220339, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI116007238, Length=205, Percent_Identity=32.6829268292683, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24584752, Length=189, Percent_Identity=32.8042328042328, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24643142, Length=181, Percent_Identity=34.2541436464088, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24644339, Length=203, Percent_Identity=29.064039408867, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24649181, Length=190, Percent_Identity=32.1052631578947, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364892, Length=200, Percent_Identity=32, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24641388, Length=260, Percent_Identity=28.4615384615385, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24640492, Length=194, Percent_Identity=29.3814432989691, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI116007234, Length=259, Percent_Identity=30.5019305019305, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24639444, Length=186, Percent_Identity=30.6451612903226, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): Y4EK_RHISN (P55434)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_443842.1
- ProteinModelPortal:   P55434
- SMR:   P55434
- GeneID:   962291
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a03840
- HOGENOM:   HBG750976
- ProtClustDB:   CLSK2484862
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PRINTS:   PR00081
- PRINTS:   PR00080

Pfam domain/function: PF00106 adh_short

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26680; Mature: 26680

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 147-147 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPMNRYEVALVTGASSGIGKAIALELASAGLRVLALGRDRAALDELHSTAGIVPVVFDL
CCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCEEEEECH
SDVSEVYGKIAGEKIDVLVNNAGLLTASASLVDLSEDDIDAMIDINIRSVFKLTRHVLKQ
HHHHHHHHHHCCCEEEEEEECCCEEEECCEEEECCCCCCCEEEECCHHHHHHHHHHHHHH
MIERRRGHIFFTGSSGGLAPHPNSSVYGATKAAVSLFSSALRCDLIGLPIRVTELFPGRV
HHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCH
ETNLYRTALGKEGAKKKLYDDNEAIQPEHMARLLRTALELPDFVDVTRLEVMPTGQVVGG
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEECC
AQMSKLSR
HHHHHCCC
>Mature Secondary Structure
MHPMNRYEVALVTGASSGIGKAIALELASAGLRVLALGRDRAALDELHSTAGIVPVVFDL
CCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCEEEEECH
SDVSEVYGKIAGEKIDVLVNNAGLLTASASLVDLSEDDIDAMIDINIRSVFKLTRHVLKQ
HHHHHHHHHHCCCEEEEEEECCCEEEECCEEEECCCCCCCEEEECCHHHHHHHHHHHHHH
MIERRRGHIFFTGSSGGLAPHPNSSVYGATKAAVSLFSSALRCDLIGLPIRVTELFPGRV
HHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCH
ETNLYRTALGKEGAKKKLYDDNEAIQPEHMARLLRTALELPDFVDVTRLEVMPTGQVVGG
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEECC
AQMSKLSR
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424