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The map label for this gene is traI

Identifier: 16519684

GI number: 16519684

Start: 534836

End: 535462

Strand: Reverse

Name: traI

Synonym: NGR_a04220

Alternate gene names: 16519684

Gene position: 535462-534836 (Counterclockwise)

Preceding gene: NA

Following gene: 16519685

Centisome position: 99.87

GC content: 58.69

Gene sequence:

>627_bases
ATGCAGATTCTCGCGATCTCAAAGCCGCGAAATATCGAAGAAGCCCAACTTCTCCGCAGCCATCACGAACTGCGCGCCCG
CGTCTTTTCCGATCGTTTGGGCTGGGAAGTCAATGTCGTCGGAGGCTGTGAGTCAGATACTTTCGACGATCTTCAACCGA
CCTACATCCTAGCCGTATCGAGCAACGATCGAGTGGTTGGATGTGCTCGCCTACTGCCTGCGCTCGGCCCCACGATGGTG
GCCAACGTTTTCCCCTCGCTCCTTTCTGCCGGTCACCTCAACGCTCATTCCTCTATGGTCGAGAGCTCGCGCTTTTGCGT
GGACACCTTCCTTGCTGAGAGCAGGGGAGACGGCTCCATTCATGAGGCGACGCTCACAATGTTCGCGGGGATCATCGAAT
GGTCGGTGGCTAACCGCTACACCGAAATCGTCACGGTGACGGATCTTCGGTTCGAACGGATCCTCGCCCGCGTCGGTTGG
CCGCTTCAGCGCATTGGCGAGCCAAGGCCGATCGGAGCGACCGTGGCCGTGGCGGGCACTCTGCCGGCAAAGGCAGACAC
ATTCATGAGGCTCCGCCCCGCCAACTACCGCTCTCAAATCATCAGCACTTTTGGCCAATCAGCGTAA

Upstream 100 bases:

>100_bases
ACGCCCAACGCGTTCCAGGGAACCTTGTAGGATCCTACAACTGCCACCCGATTCCGATTTGTGCTGATTTCCCCGCGGTC
CAACAACGACGGAGAAATTC

Downstream 100 bases:

>100_bases
GGAGAAATCAGTGGAACAGCTTCGCTCTCATCCTCGCCTCGTCCGCAAGCTTCAGGAGGCGCTTGGCGACCAGCTCTGCG
TTGCTTTGGACGATAGCAAC

Product: autoinducer synthesis protein

Products: NA

Alternate protein names: Autoinducer synthesis protein traI

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MQILAISKPRNIEEAQLLRSHHELRARVFSDRLGWEVNVVGGCESDTFDDLQPTYILAVSSNDRVVGCARLLPALGPTMV
ANVFPSLLSAGHLNAHSSMVESSRFCVDTFLAESRGDGSIHEATLTMFAGIIEWSVANRYTEIVTVTDLRFERILARVGW
PLQRIGEPRPIGATVAVAGTLPAKADTFMRLRPANYRSQIISTFGQSA

Sequences:

>Translated_208_residues
MQILAISKPRNIEEAQLLRSHHELRARVFSDRLGWEVNVVGGCESDTFDDLQPTYILAVSSNDRVVGCARLLPALGPTMV
ANVFPSLLSAGHLNAHSSMVESSRFCVDTFLAESRGDGSIHEATLTMFAGIIEWSVANRYTEIVTVTDLRFERILARVGW
PLQRIGEPRPIGATVAVAGTLPAKADTFMRLRPANYRSQIISTFGQSA
>Mature_208_residues
MQILAISKPRNIEEAQLLRSHHELRARVFSDRLGWEVNVVGGCESDTFDDLQPTYILAVSSNDRVVGCARLLPALGPTMV
ANVFPSLLSAGHLNAHSSMVESSRFCVDTFLAESRGDGSIHEATLTMFAGIIEWSVANRYTEIVTVTDLRFERILARVGW
PLQRIGEPRPIGATVAVAGTLPAKADTFMRLRPANYRSQIISTFGQSA

Specific function: Required for the synthesis of OHHL (N-(3-oxooctanoyl)-L- homoserine lactone), an autoinducer molecule which binds to traR and thus acts in the control of conjugal transfer

COG id: COG3916

COG function: function code TQ; N-acyl-L-homoserine lactone synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the autoinducer synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRAI_RHISN (P55394)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_443804.1
- ProteinModelPortal:   P55394
- SMR:   P55394
- GeneID:   962268
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a04220
- HOGENOM:   HBG368426
- ProtClustDB:   PRK13834
- GO:   GO:0000746
- InterPro:   IPR016181
- InterPro:   IPR001690
- InterPro:   IPR018311
- Gene3D:   G3DSA:3.40.630.30
- PRINTS:   PR01549

Pfam domain/function: PF00765 Autoind_synth; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.1.184

Molecular weight: Translated: 22826; Mature: 22826

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00949 AUTOINDUCER_SYNTH_1; PS51187 AUTOINDUCER_SYNTH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILAISKPRNIEEAQLLRSHHELRARVFSDRLGWEVNVVGGCESDTFDDLQPTYILAVS
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEEEC
SNDRVVGCARLLPALGPTMVANVFPSLLSAGHLNAHSSMVESSRFCVDTFLAESRGDGSI
CCCCEEHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCH
HEATLTMFAGIIEWSVANRYTEIVTVTDLRFERILARVGWPLQRIGEPRPIGATVAVAGT
HHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEECC
LPAKADTFMRLRPANYRSQIISTFGQSA
CCCCCCCEEEECCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MQILAISKPRNIEEAQLLRSHHELRARVFSDRLGWEVNVVGGCESDTFDDLQPTYILAVS
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEEEC
SNDRVVGCARLLPALGPTMVANVFPSLLSAGHLNAHSSMVESSRFCVDTFLAESRGDGSI
CCCCEEHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCH
HEATLTMFAGIIEWSVANRYTEIVTVTDLRFERILARVGWPLQRIGEPRPIGATVAVAGT
HHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEECC
LPAKADTFMRLRPANYRSQIISTFGQSA
CCCCCCCEEEECCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424