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The map label for this gene is noeL

Identifier: 16519646

GI number: 16519646

Start: 49751

End: 50806

Strand: Direct

Name: noeL

Synonym: NGR_a00410

Alternate gene names: 16519646

Gene position: 49751-50806 (Clockwise)

Preceding gene: 16519647

Following gene: 16519645

Centisome position: 9.28

GC content: 51.7

Gene sequence:

>1056_bases
GTGACAGACCGGAAAGTAGCTTTAATATCTGGCGTAACGGGTCAGGATGGGGCTTATCTTGCTGAACTGCTGTTGGACGA
AGGCTATATTGTTCACGGCATAAAGCGTCGTTCGTCATCATTCAACACTCAACGGATAGAGCACATTTATCAAGAACGTC
ACGATCCTGAAGCGAGATTTTTTCTCCACTATGGGGATATGACGGATTCGACCAATTTGCTGCGCATTGTTCAGCAAACC
CAGCCGCATGAGATCTACAACCTTGCAGCGCAAAGCCACGTTCAGGTAAGCTTCGAAACCCCCGAGTACACTGCAAACGC
CGATGCAATTGGTACATTGCGCATGCTGGAAGCTATTCGGATTCTTGGGCTGACCAATCGGACTCGCTTTTATCAGGCAT
CGACTTCAGAGCTATATGGGCTGGCTCAAGAGAGCCCCCAAAACGAAAAGACGCCATTCTACCCGCGTTCACCCTACGCG
GCCGCAAAGCTGTACGCGTACTGGATTGTCGTAAATTATAGAGAGGCTTACGGCATGCATGCCTCCAACGGTATTCTTTT
CAACCACGAAAGTCCGCTTCGTGGGGAGACGTTCGTGACTCGCAAGATCACCCGGGCTGCAGCGGCAATCAGTCTAGGTA
AACAAGAGGTCCTTTATCTTGGAAATCTAGATGCTCAACGTGACTGGGGACATGCCCGTGAGTATGTGCGGGGCATGTGG
ATGATGTGTCAACAGGATAGACCAGGCGACTATGTCCTGGCTACCGGGGTGACAACATCGGTTCGCACGTTTGTCGAATG
GGCCTTCGAGGAAACCGGAATGACGATTGAATGGGTGGGAGAAGGCATTGAAGAAAGGGGCATAGACGCCGCGACAGGCA
GATGCGTCGTTGCGGTGGATCCGCGCTATTTCAGGCCCACGGAAGTAGATTTACTTTTAGGCGATGCCACCAAGGCCCGG
CAGGTTTTGGGCTGGAGGCACGAGACAAGTGTGAGAGATCTTGCTTGCGAAATGGTAAGGGAAGATCTCTCTTATTTGAG
GGGCACCCGACAGTAA

Upstream 100 bases:

>100_bases
CCATAGGCCCGCTCTGTCGCCGAAGCACGACGATTTCCTTCACACCAGCTGCCCTAGCGCACGCCCTCTCCCGCAGGAAT
ATCACTGGAGATTCGACAAA

Downstream 100 bases:

>100_bases
GAGGCGAGATGCCGATGTATTTGCTAGACGGAAAGCGTATTTGGGTCGCAGGACACAAAGGTATGGTCGGCAGCGCCATA
ATTCGATCGCTTGCCTCCGA

Product: GDP-mannose 4,6, dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTDRKVALISGVTGQDGAYLAELLLDEGYIVHGIKRRSSSFNTQRIEHIYQERHDPEARFFLHYGDMTDSTNLLRIVQQT
QPHEIYNLAAQSHVQVSFETPEYTANADAIGTLRMLEAIRILGLTNRTRFYQASTSELYGLAQESPQNEKTPFYPRSPYA
AAKLYAYWIVVNYREAYGMHASNGILFNHESPLRGETFVTRKITRAAAAISLGKQEVLYLGNLDAQRDWGHAREYVRGMW
MMCQQDRPGDYVLATGVTTSVRTFVEWAFEETGMTIEWVGEGIEERGIDAATGRCVVAVDPRYFRPTEVDLLLGDATKAR
QVLGWRHETSVRDLACEMVREDLSYLRGTRQ

Sequences:

>Translated_351_residues
MTDRKVALISGVTGQDGAYLAELLLDEGYIVHGIKRRSSSFNTQRIEHIYQERHDPEARFFLHYGDMTDSTNLLRIVQQT
QPHEIYNLAAQSHVQVSFETPEYTANADAIGTLRMLEAIRILGLTNRTRFYQASTSELYGLAQESPQNEKTPFYPRSPYA
AAKLYAYWIVVNYREAYGMHASNGILFNHESPLRGETFVTRKITRAAAAISLGKQEVLYLGNLDAQRDWGHAREYVRGMW
MMCQQDRPGDYVLATGVTTSVRTFVEWAFEETGMTIEWVGEGIEERGIDAATGRCVVAVDPRYFRPTEVDLLLGDATKAR
QVLGWRHETSVRDLACEMVREDLSYLRGTRQ
>Mature_350_residues
TDRKVALISGVTGQDGAYLAELLLDEGYIVHGIKRRSSSFNTQRIEHIYQERHDPEARFFLHYGDMTDSTNLLRIVQQTQ
PHEIYNLAAQSHVQVSFETPEYTANADAIGTLRMLEAIRILGLTNRTRFYQASTSELYGLAQESPQNEKTPFYPRSPYAA
AKLYAYWIVVNYREAYGMHASNGILFNHESPLRGETFVTRKITRAAAAISLGKQEVLYLGNLDAQRDWGHAREYVRGMWM
MCQQDRPGDYVLATGVTTSVRTFVEWAFEETGMTIEWVGEGIEERGIDAATGRCVVAVDPRYFRPTEVDLLLGDATKARQ
VLGWRHETSVRDLACEMVREDLSYLRGTRQ

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=345, Percent_Identity=60, Blast_Score=433, Evalue=1e-121,
Organism=Escherichia coli, GI1788366, Length=349, Percent_Identity=64.4699140401146, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI48994969, Length=257, Percent_Identity=27.6264591439689, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788353, Length=208, Percent_Identity=27.4038461538462, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=346, Percent_Identity=62.4277456647399, Blast_Score=453, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI133901786, Length=348, Percent_Identity=62.0689655172414, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI133901790, Length=346, Percent_Identity=62.4277456647399, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17539422, Length=346, Percent_Identity=62.4277456647399, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI133901788, Length=346, Percent_Identity=62.4277456647399, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17507723, Length=346, Percent_Identity=62.4277456647399, Blast_Score=443, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24158427, Length=347, Percent_Identity=59.9423631123919, Blast_Score=441, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39982; Mature: 39850

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRKVALISGVTGQDGAYLAELLLDEGYIVHGIKRRSSSFNTQRIEHIYQERHDPEARF
CCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEEHHCCCCCCHHHHHHHHHHCCCCCEEE
FLHYGDMTDSTNLLRIVQQTQPHEIYNLAAQSHVQVSFETPEYTANADAIGTLRMLEAIR
EEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
ILGLTNRTRFYQASTSELYGLAQESPQNEKTPFYPRSPYAAAKLYAYWIVVNYREAYGMH
HHCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEEEEEEEEEHHHCCCC
ASNGILFNHESPLRGETFVTRKITRAAAAISLGKQEVLYLGNLDAQRDWGHAREYVRGMW
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
MMCQQDRPGDYVLATGVTTSVRTFVEWAFEETGMTIEWVGEGIEERGIDAATGRCVVAVD
HHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCEEEEEEC
PRYFRPTEVDLLLGDATKARQVLGWRHETSVRDLACEMVREDLSYLRGTRQ
CCCCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDRKVALISGVTGQDGAYLAELLLDEGYIVHGIKRRSSSFNTQRIEHIYQERHDPEARF
CCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEEHHCCCCCCHHHHHHHHHHCCCCCEEE
FLHYGDMTDSTNLLRIVQQTQPHEIYNLAAQSHVQVSFETPEYTANADAIGTLRMLEAIR
EEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
ILGLTNRTRFYQASTSELYGLAQESPQNEKTPFYPRSPYAAAKLYAYWIVVNYREAYGMH
HHCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEEEEEEEEEHHHCCCC
ASNGILFNHESPLRGETFVTRKITRAAAAISLGKQEVLYLGNLDAQRDWGHAREYVRGMW
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
MMCQQDRPGDYVLATGVTTSVRTFVEWAFEETGMTIEWVGEGIEERGIDAATGRCVVAVD
HHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCEEEEEEC
PRYFRPTEVDLLLGDATKARQVLGWRHETSVRDLACEMVREDLSYLRGTRQ
CCCCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10065558 [H]