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The map label for this gene is nolK

Identifier: 16519645

GI number: 16519645

Start: 50815

End: 51759

Strand: Direct

Name: nolK

Synonym: NGR_a00420

Alternate gene names: 16519645

Gene position: 50815-51759 (Clockwise)

Preceding gene: 16519646

Following gene: 16519644

Centisome position: 9.48

GC content: 49.52

Gene sequence:

>945_bases
ATGCCGATGTATTTGCTAGACGGAAAGCGTATTTGGGTCGCAGGACACAAAGGTATGGTCGGCAGCGCCATAATTCGATC
GCTTGCCTCCGAGGATTGCGAAGTCATCGTTGCAGATAGGCAAAAGCTTGATCTGACGCGGCAAGAGGAAGTTGAGAAAT
TTCTATTAAAGGAAAAGCCGCACGCGGTCATAATGGCGGCAGCGAAGGTGGGTGGGATCCTGGCAAATGATACTATGCCC
GCTGACTTCATCTATCAAAACCTTATCATGGAGGCTAATGTCATTGAGGGCTCCTTCCGCAGTGGCGTTGAAAAGCTTCT
TTTCCTTGGATCGAGTTGCATATATCCGAAGTATGCGGCGCAGCCCATAAGGGAAGAGGCTCTATTAACCGGACCACTTG
AGCCGACCAACGAGTGGTATGCGATCGCCAAAATCGCCGGCATTAAGTTGTGTCAAGCGTATCGTAAGCAATACGGCGCA
AACTTCATATCAGCCATGCCGACAAATCTCTATGGCCCACGCGATAAGTTCGATCTTAACTCCAGCCACGTCGTCCCTGC
CTTAATACGCAAAGCACATGAGGCAAAGATTAAAGACCTTGGGTGCTTGTCTATATGGGGAAGCGGCACACCTACTCGAG
ACTTTTTGTACAGTGAAGACTGCTCCGACGCCCTGGTCTTCCTACTTAAGCATTATTCCGAAACGGAACACATTAACATA
GGCTCCGGGGGGGAAATAAGTATCATTGAACTAGCCCACATCGTCTGCCGTGTTGTTGGTTTTAAAGGCGATATAGTCTT
CGACACATCCAAGCCGGACGGAACGCCACGAAAGCTTTTATCTAGCGAAAGACTCGTGTCGATGGGTTGGCGCCCGAAGA
CCTCGCTCGAGCTGGGACTGGCCAAATCCTATGAATCGTTTGTCAGCAATGTGGCTGATAATTAA

Upstream 100 bases:

>100_bases
TTTTGGGCTGGAGGCACGAGACAAGTGTGAGAGATCTTGCTTGCGAAATGGTAAGGGAAGATCTCTCTTATTTGAGGGGC
ACCCGACAGTAAGAGGCGAG

Downstream 100 bases:

>100_bases
GGCGGGTCGCGACTCTGGCACTGACACCGGTTTGCAAAGACGCCCCACGGCGCCGCTCCCTCCCACACCATCGGCCTGCG
GGTCACGGGCGAGTCGCTTG

Product: nucleotide sugar epimerase/dehydrogenase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPMYLLDGKRIWVAGHKGMVGSAIIRSLASEDCEVIVADRQKLDLTRQEEVEKFLLKEKPHAVIMAAAKVGGILANDTMP
ADFIYQNLIMEANVIEGSFRSGVEKLLFLGSSCIYPKYAAQPIREEALLTGPLEPTNEWYAIAKIAGIKLCQAYRKQYGA
NFISAMPTNLYGPRDKFDLNSSHVVPALIRKAHEAKIKDLGCLSIWGSGTPTRDFLYSEDCSDALVFLLKHYSETEHINI
GSGGEISIIELAHIVCRVVGFKGDIVFDTSKPDGTPRKLLSSERLVSMGWRPKTSLELGLAKSYESFVSNVADN

Sequences:

>Translated_314_residues
MPMYLLDGKRIWVAGHKGMVGSAIIRSLASEDCEVIVADRQKLDLTRQEEVEKFLLKEKPHAVIMAAAKVGGILANDTMP
ADFIYQNLIMEANVIEGSFRSGVEKLLFLGSSCIYPKYAAQPIREEALLTGPLEPTNEWYAIAKIAGIKLCQAYRKQYGA
NFISAMPTNLYGPRDKFDLNSSHVVPALIRKAHEAKIKDLGCLSIWGSGTPTRDFLYSEDCSDALVFLLKHYSETEHINI
GSGGEISIIELAHIVCRVVGFKGDIVFDTSKPDGTPRKLLSSERLVSMGWRPKTSLELGLAKSYESFVSNVADN
>Mature_313_residues
PMYLLDGKRIWVAGHKGMVGSAIIRSLASEDCEVIVADRQKLDLTRQEEVEKFLLKEKPHAVIMAAAKVGGILANDTMPA
DFIYQNLIMEANVIEGSFRSGVEKLLFLGSSCIYPKYAAQPIREEALLTGPLEPTNEWYAIAKIAGIKLCQAYRKQYGAN
FISAMPTNLYGPRDKFDLNSSHVVPALIRKAHEAKIKDLGCLSIWGSGTPTRDFLYSEDCSDALVFLLKHYSETEHINIG
SGGEISIIELAHIVCRVVGFKGDIVFDTSKPDGTPRKLLSSERLVSMGWRPKTSLELGLAKSYESFVSNVADN

Specific function: Putative nucleotide sugar epimerase/dehydrogenase

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family

Homologues:

Organism=Homo sapiens, GI4507709, Length=284, Percent_Identity=35.9154929577465, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI7657641, Length=335, Percent_Identity=23.5820895522388, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI42516563, Length=317, Percent_Identity=28.7066246056782, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1788365, Length=315, Percent_Identity=51.1111111111111, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1788353, Length=351, Percent_Identity=22.7920227920228, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17554428, Length=309, Percent_Identity=33.0097087378641, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI19922778, Length=316, Percent_Identity=31.6455696202532, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI21356223, Length=324, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y4AF_RHISN (P55353)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_443765.1
- ProteinModelPortal:   P55353
- SMR:   P55353
- GeneID:   962636
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a00420
- HOGENOM:   HBG381459
- GO:   GO:0009877
- InterPro:   IPR001509
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01370 Epimerase

EC number: 1.1.1.271

Molecular weight: Translated: 34674; Mature: 34543

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMYLLDGKRIWVAGHKGMVGSAIIRSLASEDCEVIVADRQKLDLTRQEEVEKFLLKEKP
CCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHCCCHHHHHHHHHHHHCCC
HAVIMAAAKVGGILANDTMPADFIYQNLIMEANVIEGSFRSGVEKLLFLGSSCIYPKYAA
CEEEEEHHHHCCEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHC
QPIREEALLTGPLEPTNEWYAIAKIAGIKLCQAYRKQYGANFISAMPTNLYGPRDKFDLN
CCHHHHHEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCC
SSHVVPALIRKAHEAKIKDLGCLSIWGSGTPTRDFLYSEDCSDALVFLLKHYSETEHINI
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCHHHHHHHHHHHCCCCCEEEC
GSGGEISIIELAHIVCRVVGFKGDIVFDTSKPDGTPRKLLSSERLVSMGWRPKTSLELGL
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHH
AKSYESFVSNVADN
HHHHHHHHHHHCCC
>Mature Secondary Structure 
PMYLLDGKRIWVAGHKGMVGSAIIRSLASEDCEVIVADRQKLDLTRQEEVEKFLLKEKP
CEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHCCCHHHHHHHHHHHHCCC
HAVIMAAAKVGGILANDTMPADFIYQNLIMEANVIEGSFRSGVEKLLFLGSSCIYPKYAA
CEEEEEHHHHCCEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHC
QPIREEALLTGPLEPTNEWYAIAKIAGIKLCQAYRKQYGANFISAMPTNLYGPRDKFDLN
CCHHHHHEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCC
SSHVVPALIRKAHEAKIKDLGCLSIWGSGTPTRDFLYSEDCSDALVFLLKHYSETEHINI
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCHHHHHHHHHHHCCCCCEEEC
GSGGEISIIELAHIVCRVVGFKGDIVFDTSKPDGTPRKLLSSERLVSMGWRPKTSLELGL
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHH
AKSYESFVSNVADN
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424