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The map label for this gene is potB

Identifier: 16273256

GI number: 16273256

Start: 1422185

End: 1423045

Strand: Reverse

Name: potB

Synonym: HI1346

Alternate gene names: 16273256

Gene position: 1423045-1422185 (Counterclockwise)

Preceding gene: 30995444

Following gene: 16273255

Centisome position: 77.76

GC content: 36.12

Gene sequence:

>861_bases
ATGAAGATAATCAATAATAAATTCCAGAAAATTACTGTTGCGATTATCTTCAGTTGGCTAATCTTCTTTGTGCTGATTCC
AAACTTATTGGTACTTGCCGTAAGTTTTCTGACTCGAGATGGTAGCAACTTTTATGCATTCCCAATTACTATTGAAAACT
ATACAAACTTGTTTAATCCGCTTTATGCACAAGTTGTGTGGAATTCATTGTCTATGTCGGGCATCGCCACCATTATTTGC
TTACTAATAGGTTATCCATTTGCTTTTATGATGAGTAAAATTCATCCTAAATATCGACCGCTCTTATTGTTCCTTGTGGT
TTTACCATTTTGGACAAACTCGTTAATTCGTATTTATGGAATGAAGGTATTTCTTGGCGTGAAAGGCATACTTAACACGA
TGTTAATTGATATGGGAATTTTGAGTGCGCCAATTCGTATTTTGAATACCGAAATTGCGGTAATCATTGGCTTAGTTTAT
TTACTATTGCCATTTATGATTTTGCCACTCTACTCTGCCATTGAAAAACTAGATAATCGTTTATTAGAAGCAGCACGAGA
TTTAGGCGCAAATACATTCCAACGTTTCTTCCGAGTGATTTTACCATTAACTATGCCAGGCATTATCGCGGGTTGTTTAT
TGGTATTATTGCCAGCAATGGGAATGTTTTATGTAGCAGACCTACTCGGTGGAGCAAAAGTCTTGCTTGTTGGTAACGTA
ATTAAGAGCGAATTCTTAATTTCTCGTAACTGGCCATTTGGTTCTGCAGTCAGTATTGGTTTAACTGTTCTAATGGCATT
GTTGATTTTCGTGTACTACCGCGCGAATAAACTATTGAATAGGAAAGTGGAGTTAGAATAA

Upstream 100 bases:

>100_bases
GTAAGTGAATTCTTTAATGAAGATGATCCACATATGGATCATAGCATAGGACAACGCGTAGGTATCACATGGCACGAAGG
CTGGGAGGTTGTACTCAACG

Downstream 100 bases:

>100_bases
TGAGTCGTTTCTACGTAATGCATTTATGTTTGTGGTATACGCTTATTTGTATATCCCAATTATTATTTTAGTCACTAATT
CCTTTAACAAAGATCGTTAT

Product: spermidine/putrescine ABC transporter membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKIINNKFQKITVAIIFSWLIFFVLIPNLLVLAVSFLTRDGSNFYAFPITIENYTNLFNPLYAQVVWNSLSMSGIATIIC
LLIGYPFAFMMSKIHPKYRPLLLFLVVLPFWTNSLIRIYGMKVFLGVKGILNTMLIDMGILSAPIRILNTEIAVIIGLVY
LLLPFMILPLYSAIEKLDNRLLEAARDLGANTFQRFFRVILPLTMPGIIAGCLLVLLPAMGMFYVADLLGGAKVLLVGNV
IKSEFLISRNWPFGSAVSIGLTVLMALLIFVYYRANKLLNRKVELE

Sequences:

>Translated_286_residues
MKIINNKFQKITVAIIFSWLIFFVLIPNLLVLAVSFLTRDGSNFYAFPITIENYTNLFNPLYAQVVWNSLSMSGIATIIC
LLIGYPFAFMMSKIHPKYRPLLLFLVVLPFWTNSLIRIYGMKVFLGVKGILNTMLIDMGILSAPIRILNTEIAVIIGLVY
LLLPFMILPLYSAIEKLDNRLLEAARDLGANTFQRFFRVILPLTMPGIIAGCLLVLLPAMGMFYVADLLGGAKVLLVGNV
IKSEFLISRNWPFGSAVSIGLTVLMALLIFVYYRANKLLNRKVELE
>Mature_286_residues
MKIINNKFQKITVAIIFSWLIFFVLIPNLLVLAVSFLTRDGSNFYAFPITIENYTNLFNPLYAQVVWNSLSMSGIATIIC
LLIGYPFAFMMSKIHPKYRPLLLFLVVLPFWTNSLIRIYGMKVFLGVKGILNTMLIDMGILSAPIRILNTEIAVIIGLVY
LLLPFMILPLYSAIEKLDNRLLEAARDLGANTFQRFFRVILPLTMPGIIAGCLLVLLPAMGMFYVADLLGGAKVLLVGNV
IKSEFLISRNWPFGSAVSIGLTVLMALLIFVYYRANKLLNRKVELE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1787369, Length=274, Percent_Identity=61.6788321167883, Blast_Score=353, Evalue=9e-99,
Organism=Escherichia coli, GI1787080, Length=219, Percent_Identity=43.8356164383562, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787713, Length=252, Percent_Identity=30.5555555555556, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): POTB_HAEIN (P45170)

Other databases:

- EMBL:   L42023
- PIR:   A64118
- RefSeq:   NP_439497.1
- ProteinModelPortal:   P45170
- GeneID:   950272
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1346
- NMPDR:   fig|71421.1.peg.1282
- TIGR:   HI_1346
- HOGENOM:   HBG685300
- OMA:   MIIATSF
- ProtClustDB:   PRK09497
- BioCyc:   HINF71421:HI_1346-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 32211; Mature: 32211

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe7e08d8)-; HASH(0xe467484)-; HASH(0xe3a11d4)-; HASH(0xccc4428)-; HASH(0xe47d74c)-; HASH(0xe5ca108)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure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HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800