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The map label for this gene is dacB

Identifier: 16273241

GI number: 16273241

Start: 1407458

End: 1408897

Strand: Reverse

Name: dacB

Synonym: HI1330

Alternate gene names: 16273241

Gene position: 1408897-1407458 (Counterclockwise)

Preceding gene: 16273243

Following gene: 16273233

Centisome position: 76.98

GC content: 37.71

Gene sequence:

>1440_bases
ATGAAAAAATTATCTTCAATTTCCACCGCACTTGGTTCCTTTTTACTTTCAGTGAGTTTTAGCCTTCCAACCTTCGCCAA
TATTAATGTTTCAGATTTAACACAAAAACTGCCTGAAGGCTCTAACGTAGGTTTCATCGCCAAAAATATAAATCAAAACC
AAATTATTGCTGATTATAATGGCTCAACCTTTATGCTTTCTGCAAGCACACAAAAAGTTTTTACAGCCGTTGCCGCTAAA
CTTGCATTAGACGATCAATTCCAATTTGAGACCGCACTTTTGAGTAATGGGAAAATTCAAAATGGGAATTTAGATGGCAA
CTTAATCGTGCGTTTCACAGGCGATCCTGATCTCACAAGAGGTCAGCTTTATAGCTTACTTGCCGAATTAAAAAAACAAG
GCATCAAAAAAATTAATGGCGATTTAGTATTAGATACCTCTGTTTTTTCTAGTCACGACCGAGGATTAGGTTGGATTTGG
AACGATCTCACAATGTGTTTCAACTCTCCACCAGCAGCTGCAAATATTGATAACAACTGTTTTTATGCGGAACTAGATGC
GAATAAAAATCCAGGTGAAATAGTAAAAATTAATGTGCCTGCACAATTTCCTATTCAAGTGTTCGGACAAGTTTATGTAG
CGGATAGCAATGAAGCGCCTTATTGTCAGCTCGATGTTGTTGTACACGACAATAATCGCTATCAAGTAAAAGGATGTTTA
GCCCGTCAATATAAACCTTTTGGCTTAAGTTTTGCCGTGCAAAATACGGATGCTTATGCTGCTGAGATTATTCAACGTCA
ATTAAGACAATTAGGTATTGAGTTCAATGGCAAAGTCTTGTTACCCCAAAAACCACAACAAGGTCAGCTATTAGCGAAAC
ATTTATCAAAACCATTACCAGATTTATTGAAAAAAATGATGAAAAAATCCGATAACCAAATTGCCGATTCATTATTCCGA
GCGGTGGCATTCAATTACTATAAACGCCCCGCCTCCTTTCAATTAGGCACATTAGCGGTTAAATCCATATTGCAAAAACA
AGGAATTCGTTTTGGTAATAGCATTTTAGCCGATGGTTCGGGTCTATCTCGCCATAATCTTGTGGCACCTAAAACGATGC
TATCCGTTTTAGAATACATCGCCAAAAACGAAGATAAACTACATTTAATGGAAACTTTCCCCATCGCAGGTGTAGATGGC
ACGATCAGTGGGCGAGGTGGATTAATTAGCCCGCCATTAGTGAAAAACGTGATTGCTAAAACTGGTTCTTTGAAAGGCGT
TTATAACCTAGCTGGCTTTATGACAAATGCGCGAGGGGAAAAAGTGGCATTTGTACAATTTATTAATGGCTACTCAACTG
GCGATTTAGAAAGCAAAACAAAACGTGCCCCATTAGTACAATTCGAGCGTAATTTATATAACGAACTCTATAAATATTAA

Upstream 100 bases:

>100_bases
TATCAAAAATAAACTTGAAATGAGAAAATAATTTACTCAATTTCATTCGGGGCTTATTATTGTTCGTCTAAAAAAAAATA
GCAACCATAACAAACCTAAT

Downstream 100 bases:

>100_bases
GGTGCTGTAGTAGATAACAACTAAAAATCCCATTTAGGTTAGAATAAACCAATGAGAAAAAGTCGCTTAAGTCAGCATAA
ACAAAATAAACTCATTGAAC

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKKLSSISTALGSFLLSVSFSLPTFANINVSDLTQKLPEGSNVGFIAKNINQNQIIADYNGSTFMLSASTQKVFTAVAAK
LALDDQFQFETALLSNGKIQNGNLDGNLIVRFTGDPDLTRGQLYSLLAELKKQGIKKINGDLVLDTSVFSSHDRGLGWIW
NDLTMCFNSPPAAANIDNNCFYAELDANKNPGEIVKINVPAQFPIQVFGQVYVADSNEAPYCQLDVVVHDNNRYQVKGCL
ARQYKPFGLSFAVQNTDAYAAEIIQRQLRQLGIEFNGKVLLPQKPQQGQLLAKHLSKPLPDLLKKMMKKSDNQIADSLFR
AVAFNYYKRPASFQLGTLAVKSILQKQGIRFGNSILADGSGLSRHNLVAPKTMLSVLEYIAKNEDKLHLMETFPIAGVDG
TISGRGGLISPPLVKNVIAKTGSLKGVYNLAGFMTNARGEKVAFVQFINGYSTGDLESKTKRAPLVQFERNLYNELYKY

Sequences:

>Translated_479_residues
MKKLSSISTALGSFLLSVSFSLPTFANINVSDLTQKLPEGSNVGFIAKNINQNQIIADYNGSTFMLSASTQKVFTAVAAK
LALDDQFQFETALLSNGKIQNGNLDGNLIVRFTGDPDLTRGQLYSLLAELKKQGIKKINGDLVLDTSVFSSHDRGLGWIW
NDLTMCFNSPPAAANIDNNCFYAELDANKNPGEIVKINVPAQFPIQVFGQVYVADSNEAPYCQLDVVVHDNNRYQVKGCL
ARQYKPFGLSFAVQNTDAYAAEIIQRQLRQLGIEFNGKVLLPQKPQQGQLLAKHLSKPLPDLLKKMMKKSDNQIADSLFR
AVAFNYYKRPASFQLGTLAVKSILQKQGIRFGNSILADGSGLSRHNLVAPKTMLSVLEYIAKNEDKLHLMETFPIAGVDG
TISGRGGLISPPLVKNVIAKTGSLKGVYNLAGFMTNARGEKVAFVQFINGYSTGDLESKTKRAPLVQFERNLYNELYKY
>Mature_479_residues
MKKLSSISTALGSFLLSVSFSLPTFANINVSDLTQKLPEGSNVGFIAKNINQNQIIADYNGSTFMLSASTQKVFTAVAAK
LALDDQFQFETALLSNGKIQNGNLDGNLIVRFTGDPDLTRGQLYSLLAELKKQGIKKINGDLVLDTSVFSSHDRGLGWIW
NDLTMCFNSPPAAANIDNNCFYAELDANKNPGEIVKINVPAQFPIQVFGQVYVADSNEAPYCQLDVVVHDNNRYQVKGCL
ARQYKPFGLSFAVQNTDAYAAEIIQRQLRQLGIEFNGKVLLPQKPQQGQLLAKHLSKPLPDLLKKMMKKSDNQIADSLFR
AVAFNYYKRPASFQLGTLAVKSILQKQGIRFGNSILADGSGLSRHNLVAPKTMLSVLEYIAKNEDKLHLMETFPIAGVDG
TISGRGGLISPPLVKNVIAKTGSLKGVYNLAGFMTNARGEKVAFVQFINGYSTGDLESKTKRAPLVQFERNLYNELYKY

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family

Homologues:

Organism=Escherichia coli, GI1789573, Length=454, Percent_Identity=44.0528634361233, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DACB_HAEIN (P45161)

Other databases:

- EMBL:   L42023
- PIR:   A64117
- RefSeq:   NP_439482.1
- PDB:   3A3D
- PDB:   3A3E
- PDB:   3A3F
- PDB:   3A3I
- PDBsum:   3A3D
- PDBsum:   3A3E
- PDBsum:   3A3F
- PDBsum:   3A3I
- ProteinModelPortal:   P45161
- SMR:   P45161
- MEROPS:   S13.001
- GeneID:   950252
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1330
- NMPDR:   fig|71421.1.peg.1267
- TIGR:   HI_1330
- HOGENOM:   HBG432869
- OMA:   MLLKSDN
- ProtClustDB:   PRK11113
- BioCyc:   HINF71421:HI_1330-MONOMER
- BRENDA:   3.4.16.4
- GO:   GO:0006508
- InterPro:   IPR012338
- InterPro:   IPR000667
- PRINTS:   PR00922
- TIGRFAMs:   TIGR00666

Pfam domain/function: PF02113 Peptidase_S13; SSF56601 PBP_transp_fold

EC number: =3.4.16.4

Molecular weight: Translated: 52686; Mature: 52686

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: ACT_SITE 69-69 ACT_SITE 72-72 ACT_SITE 310-310 BINDING 420-420

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSSISTALGSFLLSVSFSLPTFANINVSDLTQKLPEGSNVGFIAKNINQNQIIADYN
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEEECC
GSTFMLSASTQKVFTAVAAKLALDDQFQFETALLSNGKIQNGNLDGNLIVRFTGDPDLTR
CCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEEEEEECCCCCCCH
GQLYSLLAELKKQGIKKINGDLVLDTSVFSSHDRGLGWIWNDLTMCFNSPPAAANIDNNC
HHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCE
FYAELDANKNPGEIVKINVPAQFPIQVFGQVYVADSNEAPYCQLDVVVHDNNRYQVKGCL
EEEEECCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCEEEEEEEEECCCEEEEEEHH
ARQYKPFGLSFAVQNTDAYAAEIIQRQLRQLGIEFNGKVLLPQKPQQGQLLAKHLSKPLP
HHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCCHH
DLLKKMMKKSDNQIADSLFRAVAFNYYKRPASFQLGTLAVKSILQKQGIRFGNSILADGS
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCC
GLSRHNLVAPKTMLSVLEYIAKNEDKLHLMETFPIAGVDGTISGRGGLISPPLVKNVIAK
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
TGSLKGVYNLAGFMTNARGEKVAFVQFINGYSTGDLESKTKRAPLVQFERNLYNELYKY
CCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLSSISTALGSFLLSVSFSLPTFANINVSDLTQKLPEGSNVGFIAKNINQNQIIADYN
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEEECC
GSTFMLSASTQKVFTAVAAKLALDDQFQFETALLSNGKIQNGNLDGNLIVRFTGDPDLTR
CCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEEEEEECCCCCCCH
GQLYSLLAELKKQGIKKINGDLVLDTSVFSSHDRGLGWIWNDLTMCFNSPPAAANIDNNC
HHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCE
FYAELDANKNPGEIVKINVPAQFPIQVFGQVYVADSNEAPYCQLDVVVHDNNRYQVKGCL
EEEEECCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCEEEEEEEEECCCEEEEEEHH
ARQYKPFGLSFAVQNTDAYAAEIIQRQLRQLGIEFNGKVLLPQKPQQGQLLAKHLSKPLP
HHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCCHH
DLLKKMMKKSDNQIADSLFRAVAFNYYKRPASFQLGTLAVKSILQKQGIRFGNSILADGS
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCC
GLSRHNLVAPKTMLSVLEYIAKNEDKLHLMETFPIAGVDGTISGRGGLISPPLVKNVIAK
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
TGSLKGVYNLAGFMTNARGEKVAFVQFINGYSTGDLESKTKRAPLVQFERNLYNELYKY
CCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800