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The map label for this gene is aceF

Identifier: 16273151

GI number: 16273151

Start: 1302097

End: 1303800

Strand: Reverse

Name: aceF

Synonym: HI1232

Alternate gene names: 16273151

Gene position: 1303800-1302097 (Counterclockwise)

Preceding gene: 16273152

Following gene: 16273150

Centisome position: 71.24

GC content: 42.14

Gene sequence:

>1704_bases
ATGTCAAAACAAATTCAAATTCCTGATATTGGTAGTGATGAAGTTACCGTAACAGAAGTCATGGTAAACGTAGGCGATAC
CATTTCTGTTGATCAATCTATCATTAACGTTGAAGGCGATAAAGCTTCAATGGAAGTGCCTGCACCAGAAGCGGGTGTTG
TAAAAGAAATTTTAGTCAAAGTGGGCGATAAAGTTTCAACTGGTACTCCAATGCTTGTATTAGAAGCGGCAGGTGCTGCT
CCAGCAGCTGATGAGCCTACTGCTCCAGTAGCTGATGCGCCTACTGCACCAGTGGTTGCTACCGCACCAACGGCTTCAGC
TATTGTAGAAGTAAATGTGCCAGATATTGGTGGCGATGAAGTCAATGTAACCGAAATTATGGTTGCAGTTGGCGATACAA
TCACTGAAGAACAATCTTTAATTACCGTTGAAGGCGATAAAGCCTCAATGGAAGTTCCTGCACCATTCGGCGGTGTGGTA
AAAGAAATTTTAGTGAAATCAGGCGATAAAGTTTCAACTGGTTCATTAATTATGCGTTTTGAAGTACTAGGTGCAGCTCC
AGCAGAATCAGCATCAGCTCCAGCTTCAACATCAGCACCACAGACAGCAGCTCCTGCTACTACTGCACAAGCACCACAGG
CAGCAGCTCCTGATACTACTGCACAAGCACCACAGGCAGCAGCTCCTGATACTACTGCACAAGCAGCTCAATCAAATAAC
AACGTATCTGGATTAAGCCAAGAACAAGTAGAAGCAAGTACAGGATATGCACACGCAACACCAGTAATTCGTCGTTTAGC
TCGTGAATTTGGTGTAAATCTAGATAAAGTAAAAGGTACTGGTCGTAAAGGTCGTATCGTTAAAGAAGATATTGAAGCTT
ATGTGAAGACCGCAGTTAAAGCCTATGAAAGCGGTGCAACAGCACAAGCTACTGGCAATGGTGTAGCAAATGGTGCTGGC
TTAGGCTTATTGCCATGGCCAAAAGTTGATTTCAGTAAATTTGGCGAAATTGAAGAAGTTGAATTAAGTCGTATCAACAA
AATTTCAGGTGCTAACTTACATCGTAACTGGGTAATAATTCCACACGTCACTCACTTCGATAAAGCAGATATCACGGATT
TAGAAGCATTCCGTAAAGAACAAAATGCGTTACGAGAAAAACAAAAACTCGGTGTAAAAATCACGCCAGTTGTGTTTATT
ATGAAAGCGGTGGCAAAAGCATTAGAAGCGTATCCACGTTTCAACAGCTCAATTACCGAAGATGCTCAACGCTTAATCCT
GAAAAAATATATTAACATCGGTGTCGCAGTAGATACGCCGAATGGCTTAGTCGTGCCTGTATTCAAAAATGTGAACAAGA
AAGGCATTATCGAACTTTCACGTGAATTAATGGAAGTATCGAAAAAAGCCCGTGAAGGCAAATTGACTGCATCTGATATG
CAAGGTGGCTGTTTCACCATTTCAAGTCTTGGTGGTATTGGTACAACGCACTTTGCACCAATCGTAAATGCGCCAGAAGT
AGCTATCCTTGGTGTGTCTAAATCATCAATGGAACCAGTATGGAATGGTAAAGAATTTGCGCCACGCTTAATTCTTCCAA
TGTCATTATCATTCGACCACCGTGTCATTGATGGTGCAGATGGAGCTCGTTTCATTAGCTATCTTGGCTCTGTTTTAGCA
GATTTACGTCGCTTAGTAATGTAA

Upstream 100 bases:

>100_bases
GTTTGAATGTAGATCGTATCAATCCGCTTTATGCGTAATTGATGAAAACTGCGGTCAAAAAACATTGTGTTTTATGACCG
CACTTTAAAAGGAACAAAAA

Downstream 100 bases:

>100_bases
TCTGTAGGTGGGCTTTAAGCCCACCGATTAATGAAATCTTTGGTGGGCAGAAACCCATCCTATAAAGACCGTAAAAGTGC
GGTCAATATTTTTAACGTTT

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSKQIQIPDIGSDEVTVTEVMVNVGDTISVDQSIINVEGDKASMEVPAPEAGVVKEILVKVGDKVSTGTPMLVLEAAGAA
PAADEPTAPVADAPTAPVVATAPTASAIVEVNVPDIGGDEVNVTEIMVAVGDTITEEQSLITVEGDKASMEVPAPFGGVV
KEILVKSGDKVSTGSLIMRFEVLGAAPAESASAPASTSAPQTAAPATTAQAPQAAAPDTTAQAPQAAAPDTTAQAAQSNN
NVSGLSQEQVEASTGYAHATPVIRRLAREFGVNLDKVKGTGRKGRIVKEDIEAYVKTAVKAYESGATAQATGNGVANGAG
LGLLPWPKVDFSKFGEIEEVELSRINKISGANLHRNWVIIPHVTHFDKADITDLEAFRKEQNALREKQKLGVKITPVVFI
MKAVAKALEAYPRFNSSITEDAQRLILKKYINIGVAVDTPNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDM
QGGCFTISSLGGIGTTHFAPIVNAPEVAILGVSKSSMEPVWNGKEFAPRLILPMSLSFDHRVIDGADGARFISYLGSVLA
DLRRLVM

Sequences:

>Translated_567_residues
MSKQIQIPDIGSDEVTVTEVMVNVGDTISVDQSIINVEGDKASMEVPAPEAGVVKEILVKVGDKVSTGTPMLVLEAAGAA
PAADEPTAPVADAPTAPVVATAPTASAIVEVNVPDIGGDEVNVTEIMVAVGDTITEEQSLITVEGDKASMEVPAPFGGVV
KEILVKSGDKVSTGSLIMRFEVLGAAPAESASAPASTSAPQTAAPATTAQAPQAAAPDTTAQAPQAAAPDTTAQAAQSNN
NVSGLSQEQVEASTGYAHATPVIRRLAREFGVNLDKVKGTGRKGRIVKEDIEAYVKTAVKAYESGATAQATGNGVANGAG
LGLLPWPKVDFSKFGEIEEVELSRINKISGANLHRNWVIIPHVTHFDKADITDLEAFRKEQNALREKQKLGVKITPVVFI
MKAVAKALEAYPRFNSSITEDAQRLILKKYINIGVAVDTPNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDM
QGGCFTISSLGGIGTTHFAPIVNAPEVAILGVSKSSMEPVWNGKEFAPRLILPMSLSFDHRVIDGADGARFISYLGSVLA
DLRRLVM
>Mature_566_residues
SKQIQIPDIGSDEVTVTEVMVNVGDTISVDQSIINVEGDKASMEVPAPEAGVVKEILVKVGDKVSTGTPMLVLEAAGAAP
AADEPTAPVADAPTAPVVATAPTASAIVEVNVPDIGGDEVNVTEIMVAVGDTITEEQSLITVEGDKASMEVPAPFGGVVK
EILVKSGDKVSTGSLIMRFEVLGAAPAESASAPASTSAPQTAAPATTAQAPQAAAPDTTAQAPQAAAPDTTAQAAQSNNN
VSGLSQEQVEASTGYAHATPVIRRLAREFGVNLDKVKGTGRKGRIVKEDIEAYVKTAVKAYESGATAQATGNGVANGAGL
GLLPWPKVDFSKFGEIEEVELSRINKISGANLHRNWVIIPHVTHFDKADITDLEAFRKEQNALREKQKLGVKITPVVFIM
KAVAKALEAYPRFNSSITEDAQRLILKKYINIGVAVDTPNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQ
GGCFTISSLGGIGTTHFAPIVNAPEVAILGVSKSSMEPVWNGKEFAPRLILPMSLSFDHRVIDGADGARFISYLGSVLAD
LRRLVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains

Homologues:

Organism=Homo sapiens, GI110671329, Length=496, Percent_Identity=27.4193548387097, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI31711992, Length=436, Percent_Identity=30.7339449541284, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI19923748, Length=206, Percent_Identity=34.4660194174757, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI203098816, Length=238, Percent_Identity=31.9327731092437, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI203098753, Length=238, Percent_Identity=31.9327731092437, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI260898739, Length=143, Percent_Identity=35.6643356643357, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1786305, Length=563, Percent_Identity=66.785079928952, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=439, Percent_Identity=28.246013667426, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=447, Percent_Identity=28.4116331096197, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=455, Percent_Identity=29.010989010989, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=208, Percent_Identity=35.5769230769231, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=29.5238095238095, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=240, Percent_Identity=30.4166666666667, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=27.6548672566372, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI18859875, Length=448, Percent_Identity=29.9107142857143, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=30.2127659574468, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=30.2127659574468, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=33.953488372093, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ODP2_HAEIN (P45118)

Other databases:

- EMBL:   L42023
- PIR:   I64111
- RefSeq:   NP_439388.1
- ProteinModelPortal:   P45118
- GeneID:   949836
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1232
- NMPDR:   fig|71421.1.peg.1178
- TIGR:   HI_1232
- HOGENOM:   HBG630916
- OMA:   KIAEGDS
- ProtClustDB:   PRK11855
- BioCyc:   HINF71421:HI_1232-MONOMER
- BRENDA:   2.3.1.12
- GO:   GO:0006096
- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- Gene3D:   G3DSA:4.10.320.10
- Gene3D:   G3DSA:3.30.559.10
- TIGRFAMs:   TIGR01348

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding; SSF47005 E3_bd; SSF51230 Hybrid_motif

EC number: =2.3.1.12

Molecular weight: Translated: 59411; Mature: 59280

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: ACT_SITE 484-484 ACT_SITE 540-540 ACT_SITE 544-544

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQIQIPDIGSDEVTVTEVMVNVGDTISVDQSIINVEGDKASMEVPAPEAGVVKEILVK
CCCCCCCCCCCCCCEEHHHHHHHCCCCEECCCEEEEECCCCCEEECCCCCHHHHHHHHHH
VGDKVSTGTPMLVLEAAGAAPAADEPTAPVADAPTAPVVATAPTASAIVEVNVPDIGGDE
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCC
VNVTEIMVAVGDTITEEQSLITVEGDKASMEVPAPFGGVVKEILVKSGDKVSTGSLIMRF
CCHHHEEEHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHCCCCCCCCCEEEEE
EVLGAAPAESASAPASTSAPQTAAPATTAQAPQAAAPDTTAQAPQAAAPDTTAQAAQSNN
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
NVSGLSQEQVEASTGYAHATPVIRRLAREFGVNLDKVKGTGRKGRIVKEDIEAYVKTAVK
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEHCCCCCCCCCCHHHHHHHHHHHHHH
AYESGATAQATGNGVANGAGLGLLPWPKVDFSKFGEIEEVELSRINKISGANLHRNWVII
HHHCCCCEECCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCEEEE
PHVTHFDKADITDLEAFRKEQNALREKQKLGVKITPVVFIMKAVAKALEAYPRFNSSITE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHH
DAQRLILKKYINIGVAVDTPNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDM
HHHHHHHHHHHHCEEEEECCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
QGGCFTISSLGGIGTTHFAPIVNAPEVAILGVSKSSMEPVWNGKEFAPRLILPMSLSFDH
CCCEEEECCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCC
RVIDGADGARFISYLGSVLADLRRLVM
EEECCCCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SKQIQIPDIGSDEVTVTEVMVNVGDTISVDQSIINVEGDKASMEVPAPEAGVVKEILVK
CCCCCCCCCCCCCEEHHHHHHHCCCCEECCCEEEEECCCCCEEECCCCCHHHHHHHHHH
VGDKVSTGTPMLVLEAAGAAPAADEPTAPVADAPTAPVVATAPTASAIVEVNVPDIGGDE
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCC
VNVTEIMVAVGDTITEEQSLITVEGDKASMEVPAPFGGVVKEILVKSGDKVSTGSLIMRF
CCHHHEEEHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHCCCCCCCCCEEEEE
EVLGAAPAESASAPASTSAPQTAAPATTAQAPQAAAPDTTAQAPQAAAPDTTAQAAQSNN
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
NVSGLSQEQVEASTGYAHATPVIRRLAREFGVNLDKVKGTGRKGRIVKEDIEAYVKTAVK
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEHCCCCCCCCCCHHHHHHHHHHHHHH
AYESGATAQATGNGVANGAGLGLLPWPKVDFSKFGEIEEVELSRINKISGANLHRNWVII
HHHCCCCEECCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCEEEE
PHVTHFDKADITDLEAFRKEQNALREKQKLGVKITPVVFIMKAVAKALEAYPRFNSSITE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHH
DAQRLILKKYINIGVAVDTPNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDM
HHHHHHHHHHHHCEEEEECCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
QGGCFTISSLGGIGTTHFAPIVNAPEVAILGVSKSSMEPVWNGKEFAPRLILPMSLSFDH
CCCEEEECCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCC
RVIDGADGARFISYLGSVLADLRRLVM
EEECCCCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800