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The map label for this gene is ilvE

Identifier: 16273115

GI number: 16273115

Start: 1258603

End: 1259634

Strand: Reverse

Name: ilvE

Synonym: HI1193

Alternate gene names: 16273115

Gene position: 1259634-1258603 (Counterclockwise)

Preceding gene: 16273121

Following gene: 16273113

Centisome position: 68.83

GC content: 43.41

Gene sequence:

>1032_bases
ATGAAAGACTTAGACTGGAATAACCTTGGTTTTAGTTATATTAAAACAGATTACCGCTTCATCGCTCACTGGAAAGATGG
CAAATGGGACGAAGGAAAACTCACTACAGATAGCACATTACATATTCACGAAGGTTCAACCGCTCTTCACTACGGTCAGC
AATGTTTTGAAGGATTAAAGGCTTACCGTTGCAAAGATGGTTCAATCAACTTATTCCGCCCGCAAGCAAATGCAGAGCGT
ATGCAACGTACCGCAGACCGTCTTTTAATGCCACGCGTACCAACTGAATTATTCGTGCGTGCATGTAAAGAAGTGGTTAA
AGCTAACCAAGATTGGTTGGGTCCTTACGGTTCTGGTGCAACCTTATACTTACGTCCATTCTTAATTGGCGTGGGCGAAA
ATATCGGCGTGAAAACGGCACCAGAGTTTATTTTCTCTGTTTTTTGTTGCCCAGTGGGTGCTTACTTCAAAGGTGGTTTG
GCTCCATCAAACTTTATCACGACCGATTATGACCGTGCTGCACCAATGGGCACTGGTGGTGTGAAAGTGGGTGGGAACTA
CGCAGCAAGCTTACTTCCACACGAATTAGCCGCAGAACAAGGTACGCCAGAACGTAAATTTGCGGATGCAATCTATTTAG
ATCCAAAAACTCACACCAAAATTGAAGAAGTCGGTGCGGCAAACTTCTTTGGGATCACAAAAGACAATAAATTCATCACA
CCAAAATCAGAATCTATTTTACCAAGTATCACTAAATACTCTCTTTTACATATCGCAAAAGAGCGTTTAGGTATGGAAGC
TATTGAAGGCGATGTGTATATCGATCAACTCGATCAATTCGTAGAAGCTGGTGCGTGCGGTACAGCGGCGGTGATTACCC
CAGTGGGCGGCATTCAACACAACGGAAAATTCCACGTATTCGATTCAGAAACTGAAGTAGGCCCTGTTACCCGTCGTCTT
TATGATGAATTAACAGGCATTCAATTCGGTGATATCGAAGCACCTGAAGGCTGGATTGTGAAAGTAGAATAA

Upstream 100 bases:

>100_bases
CTAAAAATTGCATCATTTCGAGAATTTATTATTTAAATCACTTCAGGAATGGTGTAGATTAAGCGAAATTTCATTCAAAA
AATAATATTAAGGAAGTAAA

Downstream 100 bases:

>100_bases
TTTCTACCAAAAAACGAAAGTGCGGTTAAAAATCAACCGCACTTTTTTATTGATTTAATCCCCATTGGCGAGAATCTTTA
TCAAATTTAACACCTGAATG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDSTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPQANAER
MQRTADRLLMPRVPTELFVRACKEVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGL
APSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQHNGKFHVFDSETEVGPVTRRL
YDELTGIQFGDIEAPEGWIVKVE

Sequences:

>Translated_343_residues
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDSTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPQANAER
MQRTADRLLMPRVPTELFVRACKEVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGL
APSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQHNGKFHVFDSETEVGPVTRRL
YDELTGIQFGDIEAPEGWIVKVE
>Mature_343_residues
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDSTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPQANAER
MQRTADRLLMPRVPTELFVRACKEVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGL
APSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQHNGKFHVFDSETEVGPVTRRL
YDELTGIQFGDIEAPEGWIVKVE

Specific function: Acts on leucine, isoleucine and valine

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI38176287, Length=346, Percent_Identity=33.8150289017341, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI296010904, Length=346, Percent_Identity=33.8150289017341, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI296010906, Length=346, Percent_Identity=33.8150289017341, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI50658084, Length=349, Percent_Identity=31.8051575931232, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI258614015, Length=304, Percent_Identity=32.2368421052632, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI296010902, Length=346, Percent_Identity=28.6127167630058, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI296010900, Length=346, Percent_Identity=28.6127167630058, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI48994963, Length=328, Percent_Identity=29.5731707317073, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17568601, Length=359, Percent_Identity=31.7548746518106, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17565728, Length=348, Percent_Identity=30.1724137931034, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322608, Length=338, Percent_Identity=32.2485207100592, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322002, Length=345, Percent_Identity=30.4347826086957, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24641779, Length=350, Percent_Identity=30.2857142857143, Blast_Score=161, Evalue=7e-40,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ILVE_HAEIN (P54689)

Other databases:

- EMBL:   L42023
- PIR:   A64189
- RefSeq:   NP_439349.1
- ProteinModelPortal:   P54689
- SMR:   P54689
- GeneID:   950143
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1193
- NMPDR:   fig|71421.1.peg.1140
- TIGR:   HI_1193
- HOGENOM:   HBG617123
- OMA:   YIKTDYR
- ProtClustDB:   PRK13357
- BioCyc:   HINF71421:HI_1193-MONOMER
- BRENDA:   2.6.1.42
- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786
- PANTHER:   PTHR11825
- PANTHER:   PTHR11825:SF2
- PIRSF:   PIRSF006468
- TIGRFAMs:   TIGR01123

Pfam domain/function: PF01063 Aminotran_4; SSF56752 Aminotrans_IV

EC number: =2.6.1.42

Molecular weight: Translated: 37900; Mature: 37900

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDSTLHIHEGSTALHYGQQCFEGLK
CCCCCCCCCCHHHEECCEEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
AYRCKDGSINLFRPQANAERMQRTADRLLMPRVPTELFVRACKEVVKANQDWLGPYGSGA
HEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGLAPSNFITTDYDRAAPMGTGG
EEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCCCCCCCCC
VKVGGNYAASLLPHELAAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
EEECCCHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCHHHHCCCCCEEEECCCCCEEC
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQH
CCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCEEHHHHHHHHHCCCCCCEEEEECCCCCEE
NGKFHVFDSETEVGPVTRRLYDELTGIQFGDIEAPEGWIVKVE
CCEEEEECCCCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC
>Mature Secondary Structure
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDSTLHIHEGSTALHYGQQCFEGLK
CCCCCCCCCCHHHEECCEEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
AYRCKDGSINLFRPQANAERMQRTADRLLMPRVPTELFVRACKEVVKANQDWLGPYGSGA
HEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGLAPSNFITTDYDRAAPMGTGG
EEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCCCCCCCCC
VKVGGNYAASLLPHELAAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
EEECCCHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCHHHHCCCCCEEEECCCCCEEC
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQH
CCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCEEHHHHHHHHHCCCCCCEEEEECCCCCEE
NGKFHVFDSETEVGPVTRRLYDELTGIQFGDIEAPEGWIVKVE
CCEEEEECCCCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800