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The map label for this gene is gltD [H]

Identifier: 162280756

GI number: 162280756

Start: 2651177

End: 2652598

Strand: Reverse

Name: gltD [H]

Synonym: Pcryo_2164

Alternate gene names: 162280756

Gene position: 2652598-2651177 (Counterclockwise)

Preceding gene: 93006989

Following gene: 93006987

Centisome position: 86.69

GC content: 46.34

Gene sequence:

>1422_bases
ATGGCAAAACGCTTAGAAAATAGTTTTCAGTTTTTAGATGTGCCACGGCTTGAGCCGATCAAAAAAGACATCGCCACGCG
TTCAACAGAGTTTGTTGAAATTTATCAGCCGTTTGAAAGTGAATCAGTCGCTCAGCAATCGCATCGCTGTTTAGAATGTG
GCAATCCGTATTGTGAGTGGAAATGCCCCGTACATAATTACATTCCTAATTGGTTAAAATTAGCCGCTGAGGGTCAAATT
TTCCAAGCTGCAGAATTGTGTCATCAAACCAATACTTTGCCTGAAGTCTGTGGACGTGTGTGCCCGCAAGACCGCCTATG
TGAAGGCTCTTGCACACTCAATGACGGCTTTGGTGCCGTGACGATTGGCAATGTCGAGAAGTATATCAATGACACCGCTT
TTGCCCTCGGCTGGCGTCCTGATATGTCTAAAGTCATTTGGACAGACAAGAAGGTTGCCATCATTGGAGCAGGACCTGCA
GGGCTTGGCTGCGCAGATATCTTGGTCAGAAATGGCGTCAAACCCGTTATCTTTGATAAACGTCCAGAGATTGGGGGTTT
ATTAACTTTCGGTATTCCAGAGTTCAAAATGGAAAAAGACGTCATGCGTAATCGACGTGTCATCTTTGAAGGTATGGGCA
TGGAATTCCGCCTTGAGACGGAAATCGGTACTGATGTCAGTATTGATGAGCTGCTCGGCGAGTATGATGCTGTATTTATG
GGTATGGGTACTTATACCTATATGCGTGGTGGCTTTGCTGGTGAAGAGTTGGAGGGTGTGTATGACGCTCTGGATTACCT
GATTGCCAACGTCAATCGCTGTAATGATTGGGAAAAAGACTCAGGCGAGTATATCGACCTAAAAGGCAAAAAAGTCGTGG
TGTTGGGTGGTGGCGATACCGCGATGGACTGTAATCGTACCAGTATTCGTCAAGGTGCTGAGCAAGTATTCTGTGCTTAT
CGTCGTGATGAGGAAAATATGCCAGGCTCACGTCGTGAAGTGGTCAATGCACGCGAGGAGGGGGTTGAGTTCTTATTTAA
CCGCCAACCGACTGAAATAATTGGCTTAAACGGTAAGGTTAATGCGGTAAAAGTAGTAACGACGCATCTGGGCGCACCAG
ACGAAAACGGTCGTCAGTATCCTGAGCCAATTGCCAACTCAGAAGAGATTATTCCCTGTGATGCAGTGATTATGGCATTT
GGTTTCCGTCCAAGTCCGGCTGATTGGTTTGACGCGCAGCAGATTTCTATGGACAAATCTGGTCGAGTATTGGCAGCGGA
AAAACAAACCTTTAAGTTCCAAACCAATAATCCTAAGATCTTTGCCGGCGGCGATATGGTACGTGGCTCTGATTTGGTAG
TCACTGCCATTTGGGAAGGTCGTGAAGCGGCTGAAGGGATTTTAGACTACTTAGAAGTGTAG

Upstream 100 bases:

>100_bases
GTAAATGACAGCCTATATAAGTAAATTTGCCTCATTGAGGCTGCCTATATCGACTGTTGTTAGCCACTATAAAAGACCCC
TATATAAAAGAGACAATACC

Downstream 100 bases:

>100_bases
TAATTAGCCTGATTGACAGCCAAATACTCGCTTAATCATGCTTTTTTCTGGCGATATTCGAGCATGTTTTGACACTTAAC
GTCTTTTATCTTTTAATAGG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MAKRLENSFQFLDVPRLEPIKKDIATRSTEFVEIYQPFESESVAQQSHRCLECGNPYCEWKCPVHNYIPNWLKLAAEGQI
FQAAELCHQTNTLPEVCGRVCPQDRLCEGSCTLNDGFGAVTIGNVEKYINDTAFALGWRPDMSKVIWTDKKVAIIGAGPA
GLGCADILVRNGVKPVIFDKRPEIGGLLTFGIPEFKMEKDVMRNRRVIFEGMGMEFRLETEIGTDVSIDELLGEYDAVFM
GMGTYTYMRGGFAGEELEGVYDALDYLIANVNRCNDWEKDSGEYIDLKGKKVVVLGGGDTAMDCNRTSIRQGAEQVFCAY
RRDEENMPGSRREVVNAREEGVEFLFNRQPTEIIGLNGKVNAVKVVTTHLGAPDENGRQYPEPIANSEEIIPCDAVIMAF
GFRPSPADWFDAQQISMDKSGRVLAAEKQTFKFQTNNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLEV

Sequences:

>Translated_473_residues
MAKRLENSFQFLDVPRLEPIKKDIATRSTEFVEIYQPFESESVAQQSHRCLECGNPYCEWKCPVHNYIPNWLKLAAEGQI
FQAAELCHQTNTLPEVCGRVCPQDRLCEGSCTLNDGFGAVTIGNVEKYINDTAFALGWRPDMSKVIWTDKKVAIIGAGPA
GLGCADILVRNGVKPVIFDKRPEIGGLLTFGIPEFKMEKDVMRNRRVIFEGMGMEFRLETEIGTDVSIDELLGEYDAVFM
GMGTYTYMRGGFAGEELEGVYDALDYLIANVNRCNDWEKDSGEYIDLKGKKVVVLGGGDTAMDCNRTSIRQGAEQVFCAY
RRDEENMPGSRREVVNAREEGVEFLFNRQPTEIIGLNGKVNAVKVVTTHLGAPDENGRQYPEPIANSEEIIPCDAVIMAF
GFRPSPADWFDAQQISMDKSGRVLAAEKQTFKFQTNNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLEV
>Mature_472_residues
AKRLENSFQFLDVPRLEPIKKDIATRSTEFVEIYQPFESESVAQQSHRCLECGNPYCEWKCPVHNYIPNWLKLAAEGQIF
QAAELCHQTNTLPEVCGRVCPQDRLCEGSCTLNDGFGAVTIGNVEKYINDTAFALGWRPDMSKVIWTDKKVAIIGAGPAG
LGCADILVRNGVKPVIFDKRPEIGGLLTFGIPEFKMEKDVMRNRRVIFEGMGMEFRLETEIGTDVSIDELLGEYDAVFMG
MGTYTYMRGGFAGEELEGVYDALDYLIANVNRCNDWEKDSGEYIDLKGKKVVVLGGGDTAMDCNRTSIRQGAEQVFCAYR
RDEENMPGSRREVVNAREEGVEFLFNRQPTEIIGLNGKVNAVKVVTTHLGAPDENGRQYPEPIANSEEIIPCDAVIMAFG
FRPSPADWFDAQQISMDKSGRVLAAEKQTFKFQTNNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLEV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=452, Percent_Identity=29.2035398230088, Blast_Score=152, Evalue=7e-37,
Organism=Escherichia coli, GI1789606, Length=470, Percent_Identity=66.3829787234042, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=461, Percent_Identity=56.3991323210412, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI87082180, Length=457, Percent_Identity=52.9540481400438, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1788468, Length=446, Percent_Identity=28.6995515695067, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1789243, Length=470, Percent_Identity=28.936170212766, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17570289, Length=489, Percent_Identity=33.9468302658487, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI71984108, Length=462, Percent_Identity=29.8701298701299, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17543860, Length=212, Percent_Identity=25.4716981132075, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=491, Percent_Identity=32.1792260692464, Blast_Score=220, Evalue=4e-58,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=34.0816326530612, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=34.0816326530612, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24665547, Length=488, Percent_Identity=34.0163934426229, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24665543, Length=488, Percent_Identity=34.0163934426229, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24640763, Length=456, Percent_Identity=27.1929824561404, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI18858217, Length=456, Percent_Identity=27.1929824561404, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52738; Mature: 52607

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRLENSFQFLDVPRLEPIKKDIATRSTEFVEIYQPFESESVAQQSHRCLECGNPYCEW
CCCCCCCCCCEECCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
KCPVHNYIPNWLKLAAEGQIFQAAELCHQTNTLPEVCGRVCPQDRLCEGSCTLNDGFGAV
ECCHHHHCCHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEECCCCCEE
TIGNVEKYINDTAFALGWRPDMSKVIWTDKKVAIIGAGPAGLGCADILVRNGVKPVIFDK
EECCHHHHHCCCEEEEECCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHCCCCCEEECC
RPEIGGLLTFGIPEFKMEKDVMRNRRVIFEGMGMEFRLETEIGTDVSIDELLGEYDAVFM
CCCCCCEEEECCCCHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHEEEE
GMGTYTYMRGGFAGEELEGVYDALDYLIANVNRCNDWEKDSGEYIDLKGKKVVVLGGGDT
ECCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEECCCCC
AMDCNRTSIRQGAEQVFCAYRRDEENMPGSRREVVNAREEGVEFLFNRQPTEIIGLNGKV
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
NAVKVVTTHLGAPDENGRQYPEPIANSEEIIPCDAVIMAFGFRPSPADWFDAQQISMDKS
EEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHCCCCCC
GRVLAAEKQTFKFQTNNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLEV
CCEEEECCCEEEEECCCCEEEECCCEECCCCEEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKRLENSFQFLDVPRLEPIKKDIATRSTEFVEIYQPFESESVAQQSHRCLECGNPYCEW
CCCCCCCCCEECCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
KCPVHNYIPNWLKLAAEGQIFQAAELCHQTNTLPEVCGRVCPQDRLCEGSCTLNDGFGAV
ECCHHHHCCHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEECCCCCEE
TIGNVEKYINDTAFALGWRPDMSKVIWTDKKVAIIGAGPAGLGCADILVRNGVKPVIFDK
EECCHHHHHCCCEEEEECCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHCCCCCEEECC
RPEIGGLLTFGIPEFKMEKDVMRNRRVIFEGMGMEFRLETEIGTDVSIDELLGEYDAVFM
CCCCCCEEEECCCCHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHEEEE
GMGTYTYMRGGFAGEELEGVYDALDYLIANVNRCNDWEKDSGEYIDLKGKKVVVLGGGDT
ECCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEECCCCC
AMDCNRTSIRQGAEQVFCAYRRDEENMPGSRREVVNAREEGVEFLFNRQPTEIIGLNGKV
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
NAVKVVTTHLGAPDENGRQYPEPIANSEEIIPCDAVIMAFGFRPSPADWFDAQQISMDKS
EEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHCCCCCC
GRVLAAEKQTFKFQTNNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLEV
CCEEEECCCEEEEECCCCEEEECCCEECCCCEEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]