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The map label for this gene is thrS [H]

Identifier: 162227933

GI number: 162227933

Start: 1628792

End: 1630699

Strand: Direct

Name: thrS [H]

Synonym: Bcen2424_1475

Alternate gene names: 162227933

Gene position: 1628792-1630699 (Clockwise)

Preceding gene: 116689496

Following gene: 116689498

Centisome position: 46.75

GC content: 61.01

Gene sequence:

>1908_bases
ATGGTTTCGATACGCTTGCCTGACGGCTCAGTTCGACAATACGAGCATCCGGTGACAGTTGCCGAGGTTGCGGCCTCGAT
CGGTCCCGGCCTTGCGAAGGCTGCGCTTGGTGGCAAGCTCGATGGCGAGCTCGTTGATACGTCGACGGTGATCGACCGCG
ACGCGTCGCTCGCGATCGTCACGGACAAGGATGCCGACGGCCTCGACATCATCCGCCACTCGACCGCCCACTTGCTCGCG
TATGCGGTGAAGGAGCTGTATCCGGACGCGCAGGTGACGATCGGCCCGGTGATCGACAACGGTTTCTATTACGACTTCTC
GTACAACCGCCCGTTTACGCCGGAAGATCTGGAAAAGATCGAAAAGCGCATGCAGGAACTCGCGAAGAAGGACGAGCCGG
TCACGCGCCGTGTCGTGTCGCGTGACGAAGCGGCCGGTTACTTCCGCAGCATCGGCGAGAAGTACAAGGCGGAGATCATC
GAATCGATTCCGCAAAGCGACGAAATCAAGCTGTACTCGCACGGCGGCTTCACCGATCTGTGCCGCGGCCCGCACGTGCC
GTCCACCGGCAAGCTGAAGGTCTTCAAGCTGATGAAGGTCGCGGGTGCGTACTGGCGCGGCGATTCGAAGAACGAGCAGC
TGCAGCGTATCTACGGCACGGCCTGGACGAAGAAGGAAGACCAGGATCAGTACCTGCACATGCTCGAGGAGGCGGAGAAG
CGCGACCACCGCAAGCTGGGCAAGCAGCTCGACCTGTTCCACATGCAGGAAGAGTCGCCGGGCATGGTGTTCTGGCATCC
GAAGGGCTGGGCGCTGTGGCAGCAGGTCGAGCAGTACATGCGCCGCCGCGTCAACGAAGCCGGTTATCTCGAGATCAAGA
CGCCGATGATCATGGACCGCTCGCTGTGGGAAGCGTCGGGCCACTGGCAGAACTATCGCGAGAACATGTTCACGACGGAG
TCGGAGAAGCGCGACTACGCGATCAAGCCGATGAACTGCCCGGGCCACGTCCAGGTGTTCAAGCACGGCCTGCGCTCGTA
TCGCGATCTGCCGCTGCGTTACGCGGAATTCGGCTCGTGCCACCGCAACGAGGCATCGGGCGCGCTGCACGGCCTGATGC
GCGTGCGCGGCTTCGTGCAGGACGATGCGCACATCTTCTGTACGGAAGACCAATTCATCTCGGAATCGATCGCGTTTAAC
ACGCTGGCCATGAGCGTGTACAAGGACTTCGGTTTCGATCACATCGACATCAAGTTGTCGCTGCGTCCCGACCAGCGTGC
GGGTACGGACGAGACGTGGGATCGTGCCGAGCAGGGTTTGCGCGATGCACTGACGGCCTGCGGCCTTACGTGGGAAGAAT
TGCCGGGTGAGGGCGCGTTCTACGGTCCGAAGATCGAGTACCACATCAAGGATGCGCTCGGCCGCTCGTGGCAGTGCGGC
ACGCTGCAGCTCGACATGGTGTTGCCGGAGCGCCTGGGGGCCGAGTACGTCGCGGAAGACAACAGCCGCCGCCGGCCGGT
GATGCTGCACCGCGCGATCGTCGGTTCGATGGAGCGATTCCTCGGCATTTTGATCGAGCACCATGCTGGTGCAATGCCGG
TTTGGCTCGCGCCGTACCAGGCAATTGTGCTCAATATCGCCGAAAGTCAGGCCGAATATGCGCAGTCTCTGACCCAAACG
TTGCAAAAACAAGGGGTTAGAGTGGCGGCCGATTTGCGCAACGAGAAGATTAGCTATAAAATACGCGAGCACACGCTGGA
AAAGGTGCCTTATCTCCTCGTCGTGGGCGATAAGGAGCGTGATGCGCAAACGGTAGCCGTGCGTGCCCGTGGCGGCGTCG
ATCTTGGCGTAATGCCGGTCGAAGCCTTCGTTGAGCGTCTGCAGGAAGACCTGCGCTCGTTCAAGTAA

Upstream 100 bases:

>100_bases
GAAAGCGGGGCCGCATTTTTTTTGTCGCGAATTTTTTGACGCGTGAGTTCGATCTCGCGTACCTGGTCTGCCGCCCACTG
TCGGCATCACGGAGATTGCT

Downstream 100 bases:

>100_bases
CCGCCCTGGCAGCGCGGCTCGTTTTTTTAATTTTTAGAGGAAACGTAACATCGCTACTGATAAGTCGTCGCACCGCATCA
ACGGTGAAATCACTGCGCCG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MVSIRLPDGSVRQYEHPVTVAEVAASIGPGLAKAALGGKLDGELVDTSTVIDRDASLAIVTDKDADGLDIIRHSTAHLLA
YAVKELYPDAQVTIGPVIDNGFYYDFSYNRPFTPEDLEKIEKRMQELAKKDEPVTRRVVSRDEAAGYFRSIGEKYKAEII
ESIPQSDEIKLYSHGGFTDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEQLQRIYGTAWTKKEDQDQYLHMLEEAEK
RDHRKLGKQLDLFHMQEESPGMVFWHPKGWALWQQVEQYMRRRVNEAGYLEIKTPMIMDRSLWEASGHWQNYRENMFTTE
SEKRDYAIKPMNCPGHVQVFKHGLRSYRDLPLRYAEFGSCHRNEASGALHGLMRVRGFVQDDAHIFCTEDQFISESIAFN
TLAMSVYKDFGFDHIDIKLSLRPDQRAGTDETWDRAEQGLRDALTACGLTWEELPGEGAFYGPKIEYHIKDALGRSWQCG
TLQLDMVLPERLGAEYVAEDNSRRRPVMLHRAIVGSMERFLGILIEHHAGAMPVWLAPYQAIVLNIAESQAEYAQSLTQT
LQKQGVRVAADLRNEKISYKIREHTLEKVPYLLVVGDKERDAQTVAVRARGGVDLGVMPVEAFVERLQEDLRSFK

Sequences:

>Translated_635_residues
MVSIRLPDGSVRQYEHPVTVAEVAASIGPGLAKAALGGKLDGELVDTSTVIDRDASLAIVTDKDADGLDIIRHSTAHLLA
YAVKELYPDAQVTIGPVIDNGFYYDFSYNRPFTPEDLEKIEKRMQELAKKDEPVTRRVVSRDEAAGYFRSIGEKYKAEII
ESIPQSDEIKLYSHGGFTDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEQLQRIYGTAWTKKEDQDQYLHMLEEAEK
RDHRKLGKQLDLFHMQEESPGMVFWHPKGWALWQQVEQYMRRRVNEAGYLEIKTPMIMDRSLWEASGHWQNYRENMFTTE
SEKRDYAIKPMNCPGHVQVFKHGLRSYRDLPLRYAEFGSCHRNEASGALHGLMRVRGFVQDDAHIFCTEDQFISESIAFN
TLAMSVYKDFGFDHIDIKLSLRPDQRAGTDETWDRAEQGLRDALTACGLTWEELPGEGAFYGPKIEYHIKDALGRSWQCG
TLQLDMVLPERLGAEYVAEDNSRRRPVMLHRAIVGSMERFLGILIEHHAGAMPVWLAPYQAIVLNIAESQAEYAQSLTQT
LQKQGVRVAADLRNEKISYKIREHTLEKVPYLLVVGDKERDAQTVAVRARGGVDLGVMPVEAFVERLQEDLRSFK
>Mature_635_residues
MVSIRLPDGSVRQYEHPVTVAEVAASIGPGLAKAALGGKLDGELVDTSTVIDRDASLAIVTDKDADGLDIIRHSTAHLLA
YAVKELYPDAQVTIGPVIDNGFYYDFSYNRPFTPEDLEKIEKRMQELAKKDEPVTRRVVSRDEAAGYFRSIGEKYKAEII
ESIPQSDEIKLYSHGGFTDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEQLQRIYGTAWTKKEDQDQYLHMLEEAEK
RDHRKLGKQLDLFHMQEESPGMVFWHPKGWALWQQVEQYMRRRVNEAGYLEIKTPMIMDRSLWEASGHWQNYRENMFTTE
SEKRDYAIKPMNCPGHVQVFKHGLRSYRDLPLRYAEFGSCHRNEASGALHGLMRVRGFVQDDAHIFCTEDQFISESIAFN
TLAMSVYKDFGFDHIDIKLSLRPDQRAGTDETWDRAEQGLRDALTACGLTWEELPGEGAFYGPKIEYHIKDALGRSWQCG
TLQLDMVLPERLGAEYVAEDNSRRRPVMLHRAIVGSMERFLGILIEHHAGAMPVWLAPYQAIVLNIAESQAEYAQSLTQT
LQKQGVRVAADLRNEKISYKIREHTLEKVPYLLVVGDKERDAQTVAVRARGGVDLGVMPVEAFVERLQEDLRSFK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=632, Percent_Identity=39.3987341772152, Blast_Score=474, Evalue=1e-133,
Organism=Homo sapiens, GI38202255, Length=638, Percent_Identity=38.5579937304075, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI20070344, Length=651, Percent_Identity=37.173579109063, Blast_Score=408, Evalue=1e-114,
Organism=Escherichia coli, GI1788013, Length=634, Percent_Identity=58.9905362776025, Blast_Score=793, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=155, Percent_Identity=27.0967741935484, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71984184, Length=632, Percent_Identity=38.7658227848101, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71984192, Length=632, Percent_Identity=38.7658227848101, Blast_Score=474, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322112, Length=654, Percent_Identity=38.5321100917431, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=40.0503778337531, Blast_Score=305, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24583842, Length=631, Percent_Identity=39.4611727416799, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI28574141, Length=631, Percent_Identity=39.4611727416799, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24583839, Length=631, Percent_Identity=39.4611727416799, Blast_Score=478, Evalue=1e-135,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 72318; Mature: 72318

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSIRLPDGSVRQYEHPVTVAEVAASIGPGLAKAALGGKLDGELVDTSTVIDRDASLAIV
CEEEECCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCCEECCHHHCCCCCCEEEE
TDKDADGLDIIRHSTAHLLAYAVKELYPDAQVTIGPVIDNGFYYDFSYNRPFTPEDLEKI
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHH
EKRMQELAKKDEPVTRRVVSRDEAAGYFRSIGEKYKAEIIESIPQSDEIKLYSHGGFTDL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHH
CRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEQLQRIYGTAWTKKEDQDQYLHMLEEAEK
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RDHRKLGKQLDLFHMQEESPGMVFWHPKGWALWQQVEQYMRRRVNEAGYLEIKTPMIMDR
HHHHHHCCCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHCH
SLWEASGHWQNYRENMFTTESEKRDYAIKPMNCPGHVQVFKHGLRSYRDLPLRYAEFGSC
HHHHCCCCHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCC
HRNEASGALHGLMRVRGFVQDDAHIFCTEDQFISESIAFNTLAMSVYKDFGFDHIDIKLS
CCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
LRPDQRAGTDETWDRAEQGLRDALTACGLTWEELPGEGAFYGPKIEYHIKDALGRSWQCG
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCEEEEEEHHHCCCCCCCC
TLQLDMVLPERLGAEYVAEDNSRRRPVMLHRAIVGSMERFLGILIEHHAGAMPVWLAPYQ
CEEEEEECHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHH
AIVLNIAESQAEYAQSLTQTLQKQGVRVAADLRNEKISYKIREHTLEKVPYLLVVGDKER
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCEEEHHHHHHHHHCCEEEEECCCCC
DAQTVAVRARGGVDLGVMPVEAFVERLQEDLRSFK
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSIRLPDGSVRQYEHPVTVAEVAASIGPGLAKAALGGKLDGELVDTSTVIDRDASLAIV
CEEEECCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCCEECCHHHCCCCCCEEEE
TDKDADGLDIIRHSTAHLLAYAVKELYPDAQVTIGPVIDNGFYYDFSYNRPFTPEDLEKI
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHH
EKRMQELAKKDEPVTRRVVSRDEAAGYFRSIGEKYKAEIIESIPQSDEIKLYSHGGFTDL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHH
CRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEQLQRIYGTAWTKKEDQDQYLHMLEEAEK
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RDHRKLGKQLDLFHMQEESPGMVFWHPKGWALWQQVEQYMRRRVNEAGYLEIKTPMIMDR
HHHHHHCCCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHCH
SLWEASGHWQNYRENMFTTESEKRDYAIKPMNCPGHVQVFKHGLRSYRDLPLRYAEFGSC
HHHHCCCCHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCC
HRNEASGALHGLMRVRGFVQDDAHIFCTEDQFISESIAFNTLAMSVYKDFGFDHIDIKLS
CCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
LRPDQRAGTDETWDRAEQGLRDALTACGLTWEELPGEGAFYGPKIEYHIKDALGRSWQCG
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCEEEEEEHHHCCCCCCCC
TLQLDMVLPERLGAEYVAEDNSRRRPVMLHRAIVGSMERFLGILIEHHAGAMPVWLAPYQ
CEEEEEECHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHH
AIVLNIAESQAEYAQSLTQTLQKQGVRVAADLRNEKISYKIREHTLEKVPYLLVVGDKER
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCEEEHHHHHHHHHCCEEEEECCCCC
DAQTVAVRARGGVDLGVMPVEAFVERLQEDLRSFK
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA