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The map label for this gene is pheS [H]

Identifier: 162227932

GI number: 162227932

Start: 1632191

End: 1633213

Strand: Direct

Name: pheS [H]

Synonym: Bcen2424_1479

Alternate gene names: 162227932

Gene position: 1632191-1633213 (Clockwise)

Preceding gene: 116689500

Following gene: 116689502

Centisome position: 46.85

GC content: 62.76

Gene sequence:

>1023_bases
ATGGGATCTATGGATCTGGACCAGATTGTCGCCGACGCGCAGCAGTCCTTCGAACAGGCTGCCGACATCACCACGCTCGA
AAACGAGAAAGCACGATTTCTCGGCAAATCGGGTGCGCTGACCGAGTTGCTGAAGGGCCTCGGCAAGCTCGATCCCGAAG
CACGCAAGACCGAAGGCGCACGCATCAACGTCGCGAAGCAGCAGGTTGAAGCCGCGCTCACCGCCCGTCGCCAGGCACTG
GCCGACGCGCTGCTGAATCAGCGCCTCACCGCCGAAGCGATCGACGTGACGTTGCCGGGCCGCGGCGCCGGCGCAGGCAG
CCTGCACCCCGTGATGCGCACGTGGGAGCGTGTCGAACAGATTTTCGGCTCGATCGGTTTCGACGTGGCCGACGGCCCCG
AGATCGAGACCGACTGGTACAACTTCACGTCGCTGAACAGCCCGGAGAACCATCCGGCGCGTTCGATGCAGGACACCTTC
TACGTCGAAGGCAAGGATGCCGACGGCCGCCAGCTGCTGCTGCGCACGCACACAAGCCCAATGCAGGTGCGTTACGCGCG
CATGAACCGTCCGCCGATCAAGGTGATCGCGCCGGGCCGCACGTATCGCGTCGACAGCGATGCGACCCACTCGCCGATGT
TCAATCAGGTCGAGGGGCTGTGGATCGACGAAAACATCAGCTTCGCCGACCTCAAGGGCGTCTATACCGACTTCCTGAAA
AAATTCTTCGAGCGCGACGACATCCTCGTGCGCTTCCGTCCGTCGTATTTCCCGTTCACGGAACCGTCAGCCGAGATCGA
CATGATGTTCGAGCAAGGCAAGAACGCCGGCAAGTGGCTCGAGATTTCCGGCTCGGGGCAGGTGCATCCGACCGTGATCC
GCAACATGGGCCTCGACCCCGAGCGCTACATCGGTTTCGCGTTCGGCAGCGGCCTCGAGCGCCTGACGATGCTGCGCTAC
GGCGTCCAGGATCTGCGGCTGTTCTTCGAGAACGACCTGCGTTTCCTGCGCCAGTTCGCATAA

Upstream 100 bases:

>100_bases
CCGGTAGTCTCGGCCGCTGCAGCGAAAACGGGGCTCTTCCGAGCCCCTTTTTTGTTTGGTGGGCGGATTCGCTCGCCAAG
ACCGAATGACGTTGGAAATG

Downstream 100 bases:

>100_bases
CGCCGCTCGCCGCGCCGACCTGTGCCCGCGCACGCCCCCGATGGACGTTTCCGACGGGGCCCGGGCGTCGATCTAACCTG
TTTCGAACGTAGACATCCAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MGSMDLDQIVADAQQSFEQAADITTLENEKARFLGKSGALTELLKGLGKLDPEARKTEGARINVAKQQVEAALTARRQAL
ADALLNQRLTAEAIDVTLPGRGAGAGSLHPVMRTWERVEQIFGSIGFDVADGPEIETDWYNFTSLNSPENHPARSMQDTF
YVEGKDADGRQLLLRTHTSPMQVRYARMNRPPIKVIAPGRTYRVDSDATHSPMFNQVEGLWIDENISFADLKGVYTDFLK
KFFERDDILVRFRPSYFPFTEPSAEIDMMFEQGKNAGKWLEISGSGQVHPTVIRNMGLDPERYIGFAFGSGLERLTMLRY
GVQDLRLFFENDLRFLRQFA

Sequences:

>Translated_340_residues
MGSMDLDQIVADAQQSFEQAADITTLENEKARFLGKSGALTELLKGLGKLDPEARKTEGARINVAKQQVEAALTARRQAL
ADALLNQRLTAEAIDVTLPGRGAGAGSLHPVMRTWERVEQIFGSIGFDVADGPEIETDWYNFTSLNSPENHPARSMQDTF
YVEGKDADGRQLLLRTHTSPMQVRYARMNRPPIKVIAPGRTYRVDSDATHSPMFNQVEGLWIDENISFADLKGVYTDFLK
KFFERDDILVRFRPSYFPFTEPSAEIDMMFEQGKNAGKWLEISGSGQVHPTVIRNMGLDPERYIGFAFGSGLERLTMLRY
GVQDLRLFFENDLRFLRQFA
>Mature_339_residues
GSMDLDQIVADAQQSFEQAADITTLENEKARFLGKSGALTELLKGLGKLDPEARKTEGARINVAKQQVEAALTARRQALA
DALLNQRLTAEAIDVTLPGRGAGAGSLHPVMRTWERVEQIFGSIGFDVADGPEIETDWYNFTSLNSPENHPARSMQDTFY
VEGKDADGRQLLLRTHTSPMQVRYARMNRPPIKVIAPGRTYRVDSDATHSPMFNQVEGLWIDENISFADLKGVYTDFLKK
FFERDDILVRFRPSYFPFTEPSAEIDMMFEQGKNAGKWLEISGSGQVHPTVIRNMGLDPERYIGFAFGSGLERLTMLRYG
VQDLRLFFENDLRFLRQFA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=263, Percent_Identity=31.9391634980989, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI5729820, Length=290, Percent_Identity=31.0344827586207, Blast_Score=115, Evalue=8e-26,
Organism=Escherichia coli, GI1788007, Length=334, Percent_Identity=57.4850299401198, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32563657, Length=265, Percent_Identity=33.2075471698113, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17508957, Length=265, Percent_Identity=33.2075471698113, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32566635, Length=249, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=267, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6325304, Length=94, Percent_Identity=40.4255319148936, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI18858079, Length=300, Percent_Identity=30.3333333333333, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17137424, Length=249, Percent_Identity=29.3172690763052, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38325; Mature: 38194

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSMDLDQIVADAQQSFEQAADITTLENEKARFLGKSGALTELLKGLGKLDPEARKTEGA
CCCCCHHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCCHHHHHHHHHCCCCHHHHCCCC
RINVAKQQVEAALTARRQALADALLNQRLTAEAIDVTLPGRGAGAGSLHPVMRTWERVEQ
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCCCCCCHHHHHHHHHHH
IFGSIGFDVADGPEIETDWYNFTSLNSPENHPARSMQDTFYVEGKDADGRQLLLRTHTSP
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCC
MQVRYARMNRPPIKVIAPGRTYRVDSDATHSPMFNQVEGLWIDENISFADLKGVYTDFLK
CEEHHHHCCCCCEEEECCCCEEEECCCCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHH
KFFERDDILVRFRPSYFPFTEPSAEIDMMFEQGKNAGKWLEISGSGQVHPTVIRNMGLDP
HHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHCCCCH
ERYIGFAFGSGLERLTMLRYGVQDLRLFFENDLRFLRQFA
HHHEEEHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GSMDLDQIVADAQQSFEQAADITTLENEKARFLGKSGALTELLKGLGKLDPEARKTEGA
CCCCHHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCCHHHHHHHHHCCCCHHHHCCCC
RINVAKQQVEAALTARRQALADALLNQRLTAEAIDVTLPGRGAGAGSLHPVMRTWERVEQ
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCCCCCCHHHHHHHHHHH
IFGSIGFDVADGPEIETDWYNFTSLNSPENHPARSMQDTFYVEGKDADGRQLLLRTHTSP
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCC
MQVRYARMNRPPIKVIAPGRTYRVDSDATHSPMFNQVEGLWIDENISFADLKGVYTDFLK
CEEHHHHCCCCCEEEECCCCEEEECCCCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHH
KFFERDDILVRFRPSYFPFTEPSAEIDMMFEQGKNAGKWLEISGSGQVHPTVIRNMGLDP
HHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHCCCCH
ERYIGFAFGSGLERLTMLRYGVQDLRLFFENDLRFLRQFA
HHHEEEHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA