Click here to switch to the map view.

The map label for this gene is oprJ [H]

Identifier: 162149612

GI number: 162149612

Start: 3933702

End: 3935297

Strand: Direct

Name: oprJ [H]

Synonym: GDI_3851

Alternate gene names: 162149612

Gene position: 3933702-3935297 (Clockwise)

Preceding gene: 162149611

Following gene: 162149614

Centisome position: 99.73

GC content: 68.3

Gene sequence:

>1596_bases
ATGATCGTGCGATCGCTGCGCCGGACTGTCATGTCCGGGGGGGCGATGCTGTCGCTGGCGGCGTTGTCCGCCTGCGACCT
GGCGCCCACCTATCACCCACCCACCTATGTCGTGCCTGCGTCTTGGCACGGGCAGGCGCCGTTCGCCACCGCCACGCCGG
CCGATACCCAGTTGCCGTCCAACTGGTGGACCATGCTGCATGATCCGCAGCTCGACGCGCTGGAAGACAAGGCCACGGCC
GCGAACGCGGACCTGCAGGCCGCGGCCGAACGCTTCGTCCAGGCCCGCGCCATGGTCATGCAGGCCCGTGCGGACCTGCT
GCCGCATTTCGGCCTGGCCTTCGGCGCGTCGGACAACAAGCAGTCGGCGGACCGCCTGTTCCGCTACAAGGGCGCCACCA
CCGCGACTGACGAGTTCTATGGCGGCCTGGCGTCGTGGGAGCCCGACTTCTGGTCGGAGATCCGCAACCAGGTGCGCATG
GCGAAATATTCCGCCCAGGAGAAGGCGGCGGATTTCGCCGCCGCGCGGCTGAGCCTGCAGGCGGAACTGGCCAACGATTA
CGTGGCCCTGCGCGGCTATGACGCGCAGGATGCGATCTATCGCAAGTCCATCGCCTATTACGAACGCGCGGTATCGGTCA
CGCAGACGCAGCTTGCCAACCAGGCGGCGCCCAAGCTGGACCTGGCCCGTGCGCAGAACCGTCTGTACGTCACCCAGGCG
GCGGAGATGGACATCCTGGCCGAGCGCGAGGTGACCGAGCATGCGATCGCGGTGCTGACCAACAGCGCGCCGTCCAGCTT
CCATATCGCGCCGGTCGATACGCTGACCTTCACGCGGGCGGCTATTCCGGTGGGCGTGCCGTCCGACCTGCTGCAGCGGC
GCCCGGATATCGCGGCGGCGGAACGGCAGATGGCGCAGGCCAACCGCAGCATCGGCGTGTCGCGCGCGGCATTCTATCCG
CATATCTCGCTCAACGCGAATGGCGGCTTCGACGCCAACGGGTTCGACCTGGCCAACCTGGCGAACAGCATGTGGTCGTA
CGGCGCGTCGGCGTCGATGCCGATCTTCGAGGGCGGGCTGCGCCGCGCGGCGCTGCAGGCCTCGTGGTCCACCTATCGCG
AGACGCGCGACCATTACCGGTCCTCGGTCCTTTCGGCGTTTCGCGAGGTCGAGGACGGGCTGTCGCGCACCGACCGGCTG
TGGGCGGAAAACGACCGCCTGAAGGCGGCGGTGTCGGCCGCGCTGGACATGCAGAACATGACGATGACCCTGTACAAGGG
CGGCCTGTCGGCCTATCTGGACGCGATCATCGCGCAGGAGGCGGCGCTGGACGCGCAGATTTCGCAGGTGCAGGTCGAAA
CCCGCTATGTCCAGGCGGAGGTGGGCCTGATCCGCGCGCTGGGCGGCGGCTGGAACGACAGGCTGCTGCCGACGCCCGAC
CAGACGATGACGTTCTCGATCCTGCAATATGACGGGCTGCACCACCCCACGCCCGCCGGCGGCATCGACACGCCGGGCGA
CACCGATACGCGCTATGACGACCTGACGGGCGCGACGACGCTGCGCACGGGGACGGTCCCGGTTTCGGGACGGTGA

Upstream 100 bases:

>100_bases
GGATCATCAACAACCCTCCGGCCGATCTGCTGGAGGGGCAGAAGGTGCATGTGGTCCAGCCGGCCAAGGGGTATGACCAG
GCAAGCGAGGAAATCGAGGG

Downstream 100 bases:

>100_bases
CCGGGCGGCGCTGACGCCTGCATGAAGCCCGGGGCCGGCTGGTCCCGGGCTTTTTTTTGACAATCATGCCGTTTGCGAAG
GGGCCGGCTTCGATCCCATG

Product: outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MIVRSLRRTVMSGGAMLSLAALSACDLAPTYHPPTYVVPASWHGQAPFATATPADTQLPSNWWTMLHDPQLDALEDKATA
ANADLQAAAERFVQARAMVMQARADLLPHFGLAFGASDNKQSADRLFRYKGATTATDEFYGGLASWEPDFWSEIRNQVRM
AKYSAQEKAADFAAARLSLQAELANDYVALRGYDAQDAIYRKSIAYYERAVSVTQTQLANQAAPKLDLARAQNRLYVTQA
AEMDILAEREVTEHAIAVLTNSAPSSFHIAPVDTLTFTRAAIPVGVPSDLLQRRPDIAAAERQMAQANRSIGVSRAAFYP
HISLNANGGFDANGFDLANLANSMWSYGASASMPIFEGGLRRAALQASWSTYRETRDHYRSSVLSAFREVEDGLSRTDRL
WAENDRLKAAVSAALDMQNMTMTLYKGGLSAYLDAIIAQEAALDAQISQVQVETRYVQAEVGLIRALGGGWNDRLLPTPD
QTMTFSILQYDGLHHPTPAGGIDTPGDTDTRYDDLTGATTLRTGTVPVSGR

Sequences:

>Translated_531_residues
MIVRSLRRTVMSGGAMLSLAALSACDLAPTYHPPTYVVPASWHGQAPFATATPADTQLPSNWWTMLHDPQLDALEDKATA
ANADLQAAAERFVQARAMVMQARADLLPHFGLAFGASDNKQSADRLFRYKGATTATDEFYGGLASWEPDFWSEIRNQVRM
AKYSAQEKAADFAAARLSLQAELANDYVALRGYDAQDAIYRKSIAYYERAVSVTQTQLANQAAPKLDLARAQNRLYVTQA
AEMDILAEREVTEHAIAVLTNSAPSSFHIAPVDTLTFTRAAIPVGVPSDLLQRRPDIAAAERQMAQANRSIGVSRAAFYP
HISLNANGGFDANGFDLANLANSMWSYGASASMPIFEGGLRRAALQASWSTYRETRDHYRSSVLSAFREVEDGLSRTDRL
WAENDRLKAAVSAALDMQNMTMTLYKGGLSAYLDAIIAQEAALDAQISQVQVETRYVQAEVGLIRALGGGWNDRLLPTPD
QTMTFSILQYDGLHHPTPAGGIDTPGDTDTRYDDLTGATTLRTGTVPVSGR
>Mature_531_residues
MIVRSLRRTVMSGGAMLSLAALSACDLAPTYHPPTYVVPASWHGQAPFATATPADTQLPSNWWTMLHDPQLDALEDKATA
ANADLQAAAERFVQARAMVMQARADLLPHFGLAFGASDNKQSADRLFRYKGATTATDEFYGGLASWEPDFWSEIRNQVRM
AKYSAQEKAADFAAARLSLQAELANDYVALRGYDAQDAIYRKSIAYYERAVSVTQTQLANQAAPKLDLARAQNRLYVTQA
AEMDILAEREVTEHAIAVLTNSAPSSFHIAPVDTLTFTRAAIPVGVPSDLLQRRPDIAAAERQMAQANRSIGVSRAAFYP
HISLNANGGFDANGFDLANLANSMWSYGASASMPIFEGGLRRAALQASWSTYRETRDHYRSSVLSAFREVEDGLSRTDRL
WAENDRLKAAVSAALDMQNMTMTLYKGGLSAYLDAIIAQEAALDAQISQVQVETRYVQAEVGLIRALGGGWNDRLLPTPD
QTMTFSILQYDGLHHPTPAGGIDTPGDTDTRYDDLTGATTLRTGTVPVSGR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=461, Percent_Identity=27.9826464208243, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI2367348, Length=500, Percent_Identity=26, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 57843; Mature: 57843

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVRSLRRTVMSGGAMLSLAALSACDLAPTYHPPTYVVPASWHGQAPFATATPADTQLPS
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
NWWTMLHDPQLDALEDKATAANADLQAAAERFVQARAMVMQARADLLPHFGLAFGASDNK
CHHHEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCH
QSADRLFRYKGATTATDEFYGGLASWEPDFWSEIRNQVRMAKYSAQEKAADFAAARLSLQ
HHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AELANDYVALRGYDAQDAIYRKSIAYYERAVSVTQTQLANQAAPKLDLARAQNRLYVTQA
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEEC
AEMDILAEREVTEHAIAVLTNSAPSSFHIAPVDTLTFTRAAIPVGVPSDLLQRRPDIAAA
CCHHHHHHHHHHHHHHHEEECCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHCCCCHHHH
ERQMAQANRSIGVSRAAFYPHISLNANGGFDANGFDLANLANSMWSYGASASMPIFEGGL
HHHHHHHHHHCCCCHHEECCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHH
RRAALQASWSTYRETRDHYRSSVLSAFREVEDGLSRTDRLWAENDRLKAAVSAALDMQNM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
TMTLYKGGLSAYLDAIIAQEAALDAQISQVQVETRYVQAEVGLIRALGGGWNDRLLPTPD
EEEEHHCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QTMTFSILQYDGLHHPTPAGGIDTPGDTDTRYDDLTGATTLRTGTVPVSGR
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MIVRSLRRTVMSGGAMLSLAALSACDLAPTYHPPTYVVPASWHGQAPFATATPADTQLPS
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
NWWTMLHDPQLDALEDKATAANADLQAAAERFVQARAMVMQARADLLPHFGLAFGASDNK
CHHHEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCH
QSADRLFRYKGATTATDEFYGGLASWEPDFWSEIRNQVRMAKYSAQEKAADFAAARLSLQ
HHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AELANDYVALRGYDAQDAIYRKSIAYYERAVSVTQTQLANQAAPKLDLARAQNRLYVTQA
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEEC
AEMDILAEREVTEHAIAVLTNSAPSSFHIAPVDTLTFTRAAIPVGVPSDLLQRRPDIAAA
CCHHHHHHHHHHHHHHHEEECCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHCCCCHHHH
ERQMAQANRSIGVSRAAFYPHISLNANGGFDANGFDLANLANSMWSYGASASMPIFEGGL
HHHHHHHHHHCCCCHHEECCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHH
RRAALQASWSTYRETRDHYRSSVLSAFREVEDGLSRTDRLWAENDRLKAAVSAALDMQNM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
TMTLYKGGLSAYLDAIIAQEAALDAQISQVQVETRYVQAEVGLIRALGGGWNDRLLPTPD
EEEEHHCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QTMTFSILQYDGLHHPTPAGGIDTPGDTDTRYDDLTGATTLRTGTVPVSGR
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]