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The map label for this gene is vsr [H]

Identifier: 162149541

GI number: 162149541

Start: 3864624

End: 3865085

Strand: Direct

Name: vsr [H]

Synonym: GDI_3779

Alternate gene names: 162149541

Gene position: 3864624-3865085 (Clockwise)

Preceding gene: 162149538

Following gene: 162149542

Centisome position: 97.98

GC content: 58.87

Gene sequence:

>462_bases
TTGGCAGACTTCCTTGAGCCTCTGGAACGATCACGCCGCATGGGGCGGATCAGGTCACAGGACACGAAGCCAGAGATCGC
GCTCCGACGGGCATTGCACCGGCTCGGGTTACGCTTCCGCCTAGGTGGCGCAGGATTGCCCGGGCGCCCTGATATTGTAC
TACCACGCCATCGTGCCGCGGTATTCGTTCACGGTTGCTTCTGGCACCGCCATCCCGGTTGCAAGGTTGCATCGACGCCA
AAGTCGAATACTGACTTTTGGGTTGCAAGATTCGACCGAAATGTTGCCCGCGATGGGAAGGTCATGCGGGAGCTCCAGGA
GTTAGGCTGGAACGTTTTTGTAATCTGGGAGTGCGAGCTTTCCGCTAAGTCGCGCTTGAGCCCAACGGCCACCCAGTTGG
CGGCGCAAATACGCGAGAGAACTATCACCAGCATAGCCGGGGTTCTGCCACAGACTGGATGA

Upstream 100 bases:

>100_bases
CCCGTAATGTGGATCGTAAGTTGCCCCGAACAGCCAGCATGGGAAAACTGACTTTCCCTACCCATCTGTCTCTTTTGCAC
GGTCGCACGCAGGAGGCAAG

Downstream 100 bases:

>100_bases
ATGCCAGCATCGACCGCACTCGCCTCCCGCGCGCGCTACCAGACGCCGATCATATAGATGAAGGGTCACAACGAGCGCAT
CACGATGATCACAAAGTGCT

Product: repair endonuclease

Products: DNA [C]

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MADFLEPLERSRRMGRIRSQDTKPEIALRRALHRLGLRFRLGGAGLPGRPDIVLPRHRAAVFVHGCFWHRHPGCKVASTP
KSNTDFWVARFDRNVARDGKVMRELQELGWNVFVIWECELSAKSRLSPTATQLAAQIRERTITSIAGVLPQTG

Sequences:

>Translated_153_residues
MADFLEPLERSRRMGRIRSQDTKPEIALRRALHRLGLRFRLGGAGLPGRPDIVLPRHRAAVFVHGCFWHRHPGCKVASTP
KSNTDFWVARFDRNVARDGKVMRELQELGWNVFVIWECELSAKSRLSPTATQLAAQIRERTITSIAGVLPQTG
>Mature_152_residues
ADFLEPLERSRRMGRIRSQDTKPEIALRRALHRLGLRFRLGGAGLPGRPDIVLPRHRAAVFVHGCFWHRHPGCKVASTPK
SNTDFWVARFDRNVARDGKVMRELQELGWNVFVIWECELSAKSRLSPTATQLAAQIRERTITSIAGVLPQTG

Specific function: May nick specific sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=130, Percent_Identity=42.3076923076923, Blast_Score=116, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 17349; Mature: 17218

Theoretical pI: Translated: 11.79; Mature: 11.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFLEPLERSRRMGRIRSQDTKPEIALRRALHRLGLRFRLGGAGLPGRPDIVLPRHRAA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCEE
VFVHGCFWHRHPGCKVASTPKSNTDFWVARFDRNVARDGKVMRELQELGWNVFVIWECEL
EEEEEHHEECCCCCCCCCCCCCCCCEEEEECCCCHHCCCHHHHHHHHCCCCEEEEEEECC
SAKSRLSPTATQLAAQIRERTITSIAGVLPQTG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ADFLEPLERSRRMGRIRSQDTKPEIALRRALHRLGLRFRLGGAGLPGRPDIVLPRHRAA
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCEE
VFVHGCFWHRHPGCKVASTPKSNTDFWVARFDRNVARDGKVMRELQELGWNVFVIWECEL
EEEEEHHEECCCCCCCCCCCCCCCCEEEEECCCCHHCCCHHHHHHHHCCCCEEEEEEECC
SAKSRLSPTATQLAAQIRERTITSIAGVLPQTG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]