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The map label for this gene is ddeI [H]

Identifier: 162149538

GI number: 162149538

Start: 3859994

End: 3861301

Strand: Direct

Name: ddeI [H]

Synonym: GDI_3776

Alternate gene names: 162149538

Gene position: 3859994-3861301 (Clockwise)

Preceding gene: 162149537

Following gene: 162149541

Centisome position: 97.87

GC content: 59.33

Gene sequence:

>1308_bases
ATGATACCATCATGCATCGACCTATTTGCCGGTTGTGGCGGGCTCTCGCTCGGTCTTGGAGCCGCCGGTTTCTCGACCCT
GTTCGCGATCGAGGCTCATGCTGATGCATTCGATACCTACCGAGCCAACTTACTTGACAGTGGCAGGAACCGCCATAGTT
GGCCGGCATGGCTCGACAAGCGAGCGTGGCAGGCTCAGGATGTACTTGTCCATCATGAAACGGAACTTGCCGAGTTACGC
GGGAAGGTCGACTTGGTCGCCGGCGGACCACCATGCCAAGGCTTCAGCATGAATGGCCTCCGGCGGCCTGATGATCCACG
CAGCAAAATGGTGGACGTCTATCTCCATTACGTCGAAATCGTCCGTCCACGCCTAATTTTGTTGGAGAATGTGGTTGGCT
TCCGCTCGATGAAGCATCGCACAGGCGGAACTTACAGCGATTATACCAAGCGTAGGCTGGAAGCCTTTGGCTACGACACC
TGGATTGAAATCTTACGGGCTGCGGACTGGGGCGTTCCGCAGCGCAGGCCGAGGTTTGTACTCATCGCTGCACCAAAGGG
CACACTACCCGGCATTGATCCAATGCAACGCTTGCGCGTCGCCAGGAAAGCATTCCTTGTCGCACGAGGGCTTGGGCCGG
ATCATACGAGCGCCAGTGCCGCGATCTCAGACCTTGAGGAAAGCGATATGGAACCCGGACTTGACAGCGAATGGGGCCAT
CTCGGTTTCCGGGCGCTGAAGCGCTCGCGGGAAGCAGCGACTCCGTACCAGCGACTGATGAGGGTTGACAGCCTTAATCA
GCCCGGCGACCTCAGGTTGCCTAGGCACAGCACCGCCTCAGTCGGGCGGATGCGGGACATATTGGACAATTGCGAACGCG
GGGTTTGTCTGAGGCTAGTGGACCGGGAACGCCTCGGCATCAAGAAACGCTCAACCACGCCGCTCGCCCCGGACGGGCCC
GCTCCTACCATTAGCACGCTACCGGATGACTTCGTGCACTATTCCAAGCCACGGGCAATGACGGTCCGGGAGCACGCGCG
CTTGCAATCCTTCCCAGATTGGTTCTCGTTCAAGGGACCCTACACAACCGGCGGCAGCCGCCGGCGGGATGCATGCCCGA
GGTTCACTCAGGTCGGGAACGCGGTGCCACCGCTTTTGGCCGAGGCACTTGGGGAGATGCTTATGGGCCTCTTAGCCGTC
CAAAAGCGTGACGAGTCGCCTCATCTCGCGGAAAGCCTCAGTATGCTCGGCGAATACCCGACGGATGTCCGCAAAATCGT
TGACGGTGATTTCGCCGCTACCTTGTAG

Upstream 100 bases:

>100_bases
GCCCGCTTCAGCGTTGATCGACGCCGATCCGCAGCCAAGCTGAGACTTGTACAATTCGATCGTCCTGGACATTTGCTGCA
ATACGTGCGATAGCGCTGCC

Downstream 100 bases:

>100_bases
TATCCATCCCGCATCTCCCTCGACCTCCACTACATGGCCGAGAAGGTTACGCTTGCGGAGGACCTTCTGGTCATATTGAG
CACTGGACTTGCGTAACTCG

Product: cytosine-specifi methyltransferase DdeI

Products: NA

Alternate protein names: M.DdeI; Cytosine-specific methyltransferase DdeI [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIPSCIDLFAGCGGLSLGLGAAGFSTLFAIEAHADAFDTYRANLLDSGRNRHSWPAWLDKRAWQAQDVLVHHETELAELR
GKVDLVAGGPPCQGFSMNGLRRPDDPRSKMVDVYLHYVEIVRPRLILLENVVGFRSMKHRTGGTYSDYTKRRLEAFGYDT
WIEILRAADWGVPQRRPRFVLIAAPKGTLPGIDPMQRLRVARKAFLVARGLGPDHTSASAAISDLEESDMEPGLDSEWGH
LGFRALKRSREAATPYQRLMRVDSLNQPGDLRLPRHSTASVGRMRDILDNCERGVCLRLVDRERLGIKKRSTTPLAPDGP
APTISTLPDDFVHYSKPRAMTVREHARLQSFPDWFSFKGPYTTGGSRRRDACPRFTQVGNAVPPLLAEALGEMLMGLLAV
QKRDESPHLAESLSMLGEYPTDVRKIVDGDFAATL

Sequences:

>Translated_435_residues
MIPSCIDLFAGCGGLSLGLGAAGFSTLFAIEAHADAFDTYRANLLDSGRNRHSWPAWLDKRAWQAQDVLVHHETELAELR
GKVDLVAGGPPCQGFSMNGLRRPDDPRSKMVDVYLHYVEIVRPRLILLENVVGFRSMKHRTGGTYSDYTKRRLEAFGYDT
WIEILRAADWGVPQRRPRFVLIAAPKGTLPGIDPMQRLRVARKAFLVARGLGPDHTSASAAISDLEESDMEPGLDSEWGH
LGFRALKRSREAATPYQRLMRVDSLNQPGDLRLPRHSTASVGRMRDILDNCERGVCLRLVDRERLGIKKRSTTPLAPDGP
APTISTLPDDFVHYSKPRAMTVREHARLQSFPDWFSFKGPYTTGGSRRRDACPRFTQVGNAVPPLLAEALGEMLMGLLAV
QKRDESPHLAESLSMLGEYPTDVRKIVDGDFAATL
>Mature_435_residues
MIPSCIDLFAGCGGLSLGLGAAGFSTLFAIEAHADAFDTYRANLLDSGRNRHSWPAWLDKRAWQAQDVLVHHETELAELR
GKVDLVAGGPPCQGFSMNGLRRPDDPRSKMVDVYLHYVEIVRPRLILLENVVGFRSMKHRTGGTYSDYTKRRLEAFGYDT
WIEILRAADWGVPQRRPRFVLIAAPKGTLPGIDPMQRLRVARKAFLVARGLGPDHTSASAAISDLEESDMEPGLDSEWGH
LGFRALKRSREAATPYQRLMRVDSLNQPGDLRLPRHSTASVGRMRDILDNCERGVCLRLVDRERLGIKKRSTTPLAPDGP
APTISTLPDDFVHYSKPRAMTVREHARLQSFPDWFSFKGPYTTGGSRRRDACPRFTQVGNAVPPLLAEALGEMLMGLLAV
QKRDESPHLAESLSMLGEYPTDVRKIVDGDFAATL

Specific function: This methylase recognizes the double-stranded sequence CTNAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the DdeI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=190, Percent_Identity=36.3157894736842, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI4503351, Length=190, Percent_Identity=36.3157894736842, Blast_Score=90, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 48394; Mature: 48394

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPSCIDLFAGCGGLSLGLGAAGFSTLFAIEAHADAFDTYRANLLDSGRNRHSWPAWLDK
CCCHHHHHHHCCCCHHHCCCHHHHHHHEEEHHCCHHHHHHHHHHHHCCCCCCCCHHHHHH
RAWQAQDVLVHHETELAELRGKVDLVAGGPPCQGFSMNGLRRPDDPRSKMVDVYLHYVEI
HHHHHHHHHHHCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VRPRLILLENVVGFRSMKHRTGGTYSDYTKRRLEAFGYDTWIEILRAADWGVPQRRPRFV
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEE
LIAAPKGTLPGIDPMQRLRVARKAFLVARGLGPDHTSASAAISDLEESDMEPGLDSEWGH
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
LGFRALKRSREAATPYQRLMRVDSLNQPGDLRLPRHSTASVGRMRDILDNCERGVCLRLV
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEH
DRERLGIKKRSTTPLAPDGPAPTISTLPDDFVHYSKPRAMTVREHARLQSFPDWFSFKGP
HHHHHCCCCCCCCCCCCCCCCCCHHCCCHHHHHCCCCCCHHHHHHHHHHCCCCHHCCCCC
YTTGGSRRRDACPRFTQVGNAVPPLLAEALGEMLMGLLAVQKRDESPHLAESLSMLGEYP
CCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
TDVRKIVDGDFAATL
HHHHHHHCCCHHHCC
>Mature Secondary Structure
MIPSCIDLFAGCGGLSLGLGAAGFSTLFAIEAHADAFDTYRANLLDSGRNRHSWPAWLDK
CCCHHHHHHHCCCCHHHCCCHHHHHHHEEEHHCCHHHHHHHHHHHHCCCCCCCCHHHHHH
RAWQAQDVLVHHETELAELRGKVDLVAGGPPCQGFSMNGLRRPDDPRSKMVDVYLHYVEI
HHHHHHHHHHHCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VRPRLILLENVVGFRSMKHRTGGTYSDYTKRRLEAFGYDTWIEILRAADWGVPQRRPRFV
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEE
LIAAPKGTLPGIDPMQRLRVARKAFLVARGLGPDHTSASAAISDLEESDMEPGLDSEWGH
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
LGFRALKRSREAATPYQRLMRVDSLNQPGDLRLPRHSTASVGRMRDILDNCERGVCLRLV
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEH
DRERLGIKKRSTTPLAPDGPAPTISTLPDDFVHYSKPRAMTVREHARLQSFPDWFSFKGP
HHHHHCCCCCCCCCCCCCCCCCCHHCCCHHHHHCCCCCCHHHHHHHHHHCCCCHHCCCCC
YTTGGSRRRDACPRFTQVGNAVPPLLAEALGEMLMGLLAVQKRDESPHLAESLSMLGEYP
CCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
TDVRKIVDGDFAATL
HHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2823226 [H]