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The map label for this gene is arsB [H]

Identifier: 162149412

GI number: 162149412

Start: 3727878

End: 3729182

Strand: Direct

Name: arsB [H]

Synonym: GDI_3650

Alternate gene names: 162149412

Gene position: 3727878-3729182 (Clockwise)

Preceding gene: 162149411

Following gene: 162149413

Centisome position: 94.52

GC content: 69.66

Gene sequence:

>1305_bases
ATGCTGGCGCTTGCGATCTTTCTGGTCACGCTGATCCTCGTCATCTGGCAGCCCCGGCTTCCCGGGCAGCCGAGGGGGCT
GGGCATCGGGTGGAGCGCGCTGGGCGGGGCGGGGGTGGCGTGGGCGGCGGGCGTCATCCACGCGCGGGACATTCCGGTGG
TCTGGCATATCGTCTGGAATGCGACCTTCACCTTCATCGCGCTGATCGTCATCTCGCTGCTGCTGGACCGGGCCGGGTTC
TTCCAGTGGGCGGCGCTTCATATCGCGCGCTGGGGCGGCGGGCGGGGGCGGGTGCTGTTCCCGCTGGTCGTGGGGCTGGG
GGCGGCGACGGCCGCGATCTTCGCCAATGACGGGGCGGCGCTGCTGCTGACGCCCATCGTCATCGCCATCCTGGCGTCGC
TGCGGCTGTCGCACGCGGCATCGCTGGCCTTCGTGATCGCGACCGGCTTCGTCGCGGACACGACCAGCCTGCCGCTGGTG
ATTTCCAATCTGGTCAACATCGTCAGCGCCGGCTTCTTCGCCGTGCCGTTCGACCGCTATGCCGCCGTCATGGTGCCGGT
GGACATGGTGTCGCTGGCCGCGACGTTCGGCGTGCTGTGGCTGTATTTTCGCCGGGACATACCGCGACACTATGCCGTCG
CGACCCTGCCGGAACCGGCGGGCGCCATTCGCGATCCGATGGTGTTCCGGGCCGCGTTTCCGCTGCTGGCGGCGCTGCTG
GTCGCCTACTTCGTCACCGCGCCGTTTCATATCCCGGTCTGCGTGGTGGCTGGGCTGGCGGCGCTGGTGCTGCTGGCCCT
GGCGGCGGGCGGTCGGGTCATTCCGGTGCGCGCGGTGCTGTGGGGGGCGCCGTGGCAGATCGTGCTGTTCAGCCTGGGCA
TGTATCTGGTGGTCTATGGGCTGCGGAATGCCGGCCTGACGGCCCATGTGGCGGACGCGCTGGTGTGGCTGGGGCACCGG
GGCACGATCGTCGCCACCCTCGGGACCGGGGTCCTGGCCGCGCTGCTGTCGTCGGGACTGAACAACATGCCCAGCGTGCT
GGTCGGTGCGCTGGCGATCCAGCAGGCGCAGGACATCGCGCCGCAGACGCGCGAACTGATGGTCTACGCCAATATCGTCG
GCTGCGACCTGGGGCCGAAATTCACCCCGATCGGCAGCCTGGCGACCCTGTTATGGCTGCATGTGCTGGCCGGAAAGGGG
CTGCGCATCGGCTGGGGGCAGTATATGCGCGTCGGCCTGGCGATCACCCCGCCGGTCCTGCTGGCCGCGCTGCTGGCGCT
GGCGGTGTGGCTGCCCGTGCTGTGA

Upstream 100 bases:

>100_bases
TCCTGATCAACCGGCCGATCGTCGTGACGCCGCTGGGGGTCAGGCTGTGCCGCCCGGCCGAGCGCGTCCTGGACATCCTG
CCGCCGCGCCAGGGCGCGTG

Downstream 100 bases:

>100_bases
ATGCGGGCCTTGCGTCCCGCCCCCTCCGCGCCGTATCGGCATGGGATGGGTTTGCGAAGGGGGCGGGCATGGACGGCATG
GGTGCGATCGATCCGGGGGG

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MLALAIFLVTLILVIWQPRLPGQPRGLGIGWSALGGAGVAWAAGVIHARDIPVVWHIVWNATFTFIALIVISLLLDRAGF
FQWAALHIARWGGGRGRVLFPLVVGLGAATAAIFANDGAALLLTPIVIAILASLRLSHAASLAFVIATGFVADTTSLPLV
ISNLVNIVSAGFFAVPFDRYAAVMVPVDMVSLAATFGVLWLYFRRDIPRHYAVATLPEPAGAIRDPMVFRAAFPLLAALL
VAYFVTAPFHIPVCVVAGLAALVLLALAAGGRVIPVRAVLWGAPWQIVLFSLGMYLVVYGLRNAGLTAHVADALVWLGHR
GTIVATLGTGVLAALLSSGLNNMPSVLVGALAIQQAQDIAPQTRELMVYANIVGCDLGPKFTPIGSLATLLWLHVLAGKG
LRIGWGQYMRVGLAITPPVLLAALLALAVWLPVL

Sequences:

>Translated_434_residues
MLALAIFLVTLILVIWQPRLPGQPRGLGIGWSALGGAGVAWAAGVIHARDIPVVWHIVWNATFTFIALIVISLLLDRAGF
FQWAALHIARWGGGRGRVLFPLVVGLGAATAAIFANDGAALLLTPIVIAILASLRLSHAASLAFVIATGFVADTTSLPLV
ISNLVNIVSAGFFAVPFDRYAAVMVPVDMVSLAATFGVLWLYFRRDIPRHYAVATLPEPAGAIRDPMVFRAAFPLLAALL
VAYFVTAPFHIPVCVVAGLAALVLLALAAGGRVIPVRAVLWGAPWQIVLFSLGMYLVVYGLRNAGLTAHVADALVWLGHR
GTIVATLGTGVLAALLSSGLNNMPSVLVGALAIQQAQDIAPQTRELMVYANIVGCDLGPKFTPIGSLATLLWLHVLAGKG
LRIGWGQYMRVGLAITPPVLLAALLALAVWLPVL
>Mature_434_residues
MLALAIFLVTLILVIWQPRLPGQPRGLGIGWSALGGAGVAWAAGVIHARDIPVVWHIVWNATFTFIALIVISLLLDRAGF
FQWAALHIARWGGGRGRVLFPLVVGLGAATAAIFANDGAALLLTPIVIAILASLRLSHAASLAFVIATGFVADTTSLPLV
ISNLVNIVSAGFFAVPFDRYAAVMVPVDMVSLAATFGVLWLYFRRDIPRHYAVATLPEPAGAIRDPMVFRAAFPLLAALL
VAYFVTAPFHIPVCVVAGLAALVLLALAAGGRVIPVRAVLWGAPWQIVLFSLGMYLVVYGLRNAGLTAHVADALVWLGHR
GTIVATLGTGVLAALLSSGLNNMPSVLVGALAIQQAQDIAPQTRELMVYANIVGCDLGPKFTPIGSLATLLWLHVLAGKG
LRIGWGQYMRVGLAITPPVLLAALLALAVWLPVL

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=431, Percent_Identity=59.6287703016241, Blast_Score=467, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45717; Mature: 45717

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALAIFLVTLILVIWQPRLPGQPRGLGIGWSALGGAGVAWAAGVIHARDIPVVWHIVWN
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCEEEHHHHH
ATFTFIALIVISLLLDRAGFFQWAALHIARWGGGRGRVLFPLVVGLGAATAAIFANDGAA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEECCCCH
LLLTPIVIAILASLRLSHAASLAFVIATGFVADTTSLPLVISNLVNIVSAGFFAVPFDRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECHHHH
AAVMVPVDMVSLAATFGVLWLYFRRDIPRHYAVATLPEPAGAIRDPMVFRAAFPLLAALL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHH
VAYFVTAPFHIPVCVVAGLAALVLLALAAGGRVIPVRAVLWGAPWQIVLFSLGMYLVVYG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHH
LRNAGLTAHVADALVWLGHRGTIVATLGTGVLAALLSSGLNNMPSVLVGALAIQQAQDIA
HCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
PQTRELMVYANIVGCDLGPKFTPIGSLATLLWLHVLAGKGLRIGWGQYMRVGLAITPPVL
CCHHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEECCHHHHHCCEEECCHHH
LAALLALAVWLPVL
HHHHHHHHHHHCCC
>Mature Secondary Structure
MLALAIFLVTLILVIWQPRLPGQPRGLGIGWSALGGAGVAWAAGVIHARDIPVVWHIVWN
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCEEEHHHHH
ATFTFIALIVISLLLDRAGFFQWAALHIARWGGGRGRVLFPLVVGLGAATAAIFANDGAA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEECCCCH
LLLTPIVIAILASLRLSHAASLAFVIATGFVADTTSLPLVISNLVNIVSAGFFAVPFDRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECHHHH
AAVMVPVDMVSLAATFGVLWLYFRRDIPRHYAVATLPEPAGAIRDPMVFRAAFPLLAALL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHH
VAYFVTAPFHIPVCVVAGLAALVLLALAAGGRVIPVRAVLWGAPWQIVLFSLGMYLVVYG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHH
LRNAGLTAHVADALVWLGHRGTIVATLGTGVLAALLSSGLNNMPSVLVGALAIQQAQDIA
HCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
PQTRELMVYANIVGCDLGPKFTPIGSLATLLWLHVLAGKGLRIGWGQYMRVGLAITPPVL
CCHHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEECCHHHHHCCEEECCHHH
LAALLALAVWLPVL
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]