Click here to switch to the map view.

The map label for this gene is istB [H]

Identifier: 162149341

GI number: 162149341

Start: 3666724

End: 3667362

Strand: Direct

Name: istB [H]

Synonym: GDI_3574

Alternate gene names: 162149341

Gene position: 3666724-3667362 (Clockwise)

Preceding gene: 162149339

Following gene: 162149342

Centisome position: 92.97

GC content: 59.15

Gene sequence:

>639_bases
TTGAAGGCGGAACTCGCCGAGCGAGAGGTGCGCTCCATCGCCTATCAAACCAAGACTGCCAGGTTCCCGGCCTACAAAGA
TTTGGCAGGGTTCGATTTCTCGGCCGCCGAGGTCAACGAGGCCATGGTCCGTCAACTCCATGCCGGGGATTTCATCGACC
GTGCCGACAACGTCGTCCTCATTGGTGGACCAGGAACCGGCAAGACCCATCTGGCCACCGCACTTGCCGTGCAGGCGATC
GAACATCACCGCAAGAAGATACGGTTCTGGTCCACGGTCGACCTCGTCAACGCCCTCGAACAGGAAAAAACCGCCAATCG
CGCAGGACAGATCGCGGAACGTCTCCTGCGCCTCGATCTCGTGATCCTGGACGAACTTGGCTATTTGCCGTTCAGCGCAT
CAGGCGGTGCCCTGCTGTTCCATCTCCTCAGCCGTCTCTACGAGCGCACCAGCGTCATCATCACCACCAATCTGAGCTTC
AGCGAATGGGGCGAAGTCTTCGGTGATCCCAAAATGACGACAGCCCTGCTCGATCGCCTTACCCACCACTGTCATATCCT
CGAAACCGGAAATGACAGCTACCGGTTCCGCGCAAGCTCCGCCGCCCCCAGGAACCGGAAGGAAAAGGCAACCGCTTGA

Upstream 100 bases:

>100_bases
TGCGCGGCCTGCGGATGTATGGCATGGCCCAGGCCACGGCCGAACTGACCGAACAGGTGCGCCGGCATTCGAGGCCGCCA
TCCCCGTCCTCTCCCAGCTT

Downstream 100 bases:

>100_bases
CCAGCCCATAAAACATAGCAGAGATAACCAAAGGCCGGGTCAGTTCTCGATGAAAACCCAGGGTCAAATCTCAGCAGAAA
TTAACACTGATGGGGGATTA

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKAELAEREVRSIAYQTKTARFPAYKDLAGFDFSAAEVNEAMVRQLHAGDFIDRADNVVLIGGPGTGKTHLATALAVQAI
EHHRKKIRFWSTVDLVNALEQEKTANRAGQIAERLLRLDLVILDELGYLPFSASGGALLFHLLSRLYERTSVIITTNLSF
SEWGEVFGDPKMTTALLDRLTHHCHILETGNDSYRFRASSAAPRNRKEKATA

Sequences:

>Translated_212_residues
MKAELAEREVRSIAYQTKTARFPAYKDLAGFDFSAAEVNEAMVRQLHAGDFIDRADNVVLIGGPGTGKTHLATALAVQAI
EHHRKKIRFWSTVDLVNALEQEKTANRAGQIAERLLRLDLVILDELGYLPFSASGGALLFHLLSRLYERTSVIITTNLSF
SEWGEVFGDPKMTTALLDRLTHHCHILETGNDSYRFRASSAAPRNRKEKATA
>Mature_212_residues
MKAELAEREVRSIAYQTKTARFPAYKDLAGFDFSAAEVNEAMVRQLHAGDFIDRADNVVLIGGPGTGKTHLATALAVQAI
EHHRKKIRFWSTVDLVNALEQEKTANRAGQIAERLLRLDLVILDELGYLPFSASGGALLFHLLSRLYERTSVIITTNLSF
SEWGEVFGDPKMTTALLDRLTHHCHILETGNDSYRFRASSAAPRNRKEKATA

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 23610; Mature: 23610

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAELAEREVRSIAYQTKTARFPAYKDLAGFDFSAAEVNEAMVRQLHAGDFIDRADNVVL
CCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEE
IGGPGTGKTHLATALAVQAIEHHRKKIRFWSTVDLVNALEQEKTANRAGQIAERLLRLDL
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VILDELGYLPFSASGGALLFHLLSRLYERTSVIITTNLSFSEWGEVFGDPKMTTALLDRL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHCCCHHHHHHHHHH
THHCHILETGNDSYRFRASSAAPRNRKEKATA
HHHEEEEECCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKAELAEREVRSIAYQTKTARFPAYKDLAGFDFSAAEVNEAMVRQLHAGDFIDRADNVVL
CCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEE
IGGPGTGKTHLATALAVQAIEHHRKKIRFWSTVDLVNALEQEKTANRAGQIAERLLRLDL
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VILDELGYLPFSASGGALLFHLLSRLYERTSVIITTNLSFSEWGEVFGDPKMTTALLDRL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHCCCHHHHHHHHHH
THHCHILETGNDSYRFRASSAAPRNRKEKATA
HHHEEEEECCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]