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The map label for this gene is greA [H]

Identifier: 162149110

GI number: 162149110

Start: 3436042

End: 3436515

Strand: Direct

Name: greA [H]

Synonym: GDI_3340

Alternate gene names: 162149110

Gene position: 3436042-3436515 (Clockwise)

Preceding gene: 162149109

Following gene: 162149111

Centisome position: 87.12

GC content: 64.14

Gene sequence:

>474_bases
TTGCAGAAATTTCCGATGACAGGCAAGGGCCTGCAGCGGCTGGAAGAAGAACTTCGCAAGCTCAAGAGTGAAGAACGCCC
GGCCGTCATACGTGCCATCGCCGAGGCCCGCAGCCACGGCGACCTTTCGGAAAACGCCGAATACCACGCGGCGCGGGACC
GCCAGTCCTTCATCGAGGGCCGGATTCTTGAACTTGAGGACATCATCTCGTCGGCCGAGGTGATCGATCCGGCGAGCCTG
TCGGGCGACCAGGTGAAATTCGGCGCCCAGGTCAGCCTGGTGGACGAGGAAACCGACAAGGAAGCCACCTACCAGATCGT
CGGCGTGCACGAGGCCGACATCAAGCAGGGGCTGCTGTCGGTGTCCTCGCCGCTGGCCAAGTCCCTGATCGGCAAGCGGA
TCGGCGATTCCGTCTCGGTCCCCGCCCCGGGCGGCGACCGGACGTACGAGATCCTGGCCGTCACCTACGGCTAG

Upstream 100 bases:

>100_bases
TTCCTGCCGGCCCCGCACCCGGCGCATTCGGTCTTGTGGGATGCTCTTCTTCAACCTTTGATGTCAGTCGCGCGACTGAC
CTTCATTCCGGGGACGCGCC

Downstream 100 bases:

>100_bases
GCGGGATGCGATGATCACCCTCAACGACGTTCGCGCCGCCGCCGCGCGGATCGAGGGGCGTGTCCTGCGCACGCCCACCG
TTCCCTCGCACGCGCTTTCC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MQKFPMTGKGLQRLEEELRKLKSEERPAVIRAIAEARSHGDLSENAEYHAARDRQSFIEGRILELEDIISSAEVIDPASL
SGDQVKFGAQVSLVDEETDKEATYQIVGVHEADIKQGLLSVSSPLAKSLIGKRIGDSVSVPAPGGDRTYEILAVTYG

Sequences:

>Translated_157_residues
MQKFPMTGKGLQRLEEELRKLKSEERPAVIRAIAEARSHGDLSENAEYHAARDRQSFIEGRILELEDIISSAEVIDPASL
SGDQVKFGAQVSLVDEETDKEATYQIVGVHEADIKQGLLSVSSPLAKSLIGKRIGDSVSVPAPGGDRTYEILAVTYG
>Mature_157_residues
MQKFPMTGKGLQRLEEELRKLKSEERPAVIRAIAEARSHGDLSENAEYHAARDRQSFIEGRILELEDIISSAEVIDPASL
SGDQVKFGAQVSLVDEETDKEATYQIVGVHEADIKQGLLSVSSPLAKSLIGKRIGDSVSVPAPGGDRTYEILAVTYG

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=52.2292993630573, Blast_Score=162, Evalue=1e-41,
Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=32.6797385620915, Blast_Score=87, Evalue=5e-19,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17093; Mature: 17093

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKFPMTGKGLQRLEEELRKLKSEERPAVIRAIAEARSHGDLSENAEYHAARDRQSFIEG
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
RILELEDIISSAEVIDPASLSGDQVKFGAQVSLVDEETDKEATYQIVGVHEADIKQGLLS
CHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEECCCCCCCCEEEEEECCHHHHHHHHHH
VSSPLAKSLIGKRIGDSVSVPAPGGDRTYEILAVTYG
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MQKFPMTGKGLQRLEEELRKLKSEERPAVIRAIAEARSHGDLSENAEYHAARDRQSFIEG
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
RILELEDIISSAEVIDPASLSGDQVKFGAQVSLVDEETDKEATYQIVGVHEADIKQGLLS
CHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEECCCCCCCCEEEEEECCHHHHHHHHHH
VSSPLAKSLIGKRIGDSVSVPAPGGDRTYEILAVTYG
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA