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The map label for this gene is guaD [H]

Identifier: 162148937

GI number: 162148937

Start: 3254984

End: 3256333

Strand: Direct

Name: guaD [H]

Synonym: GDI_3167

Alternate gene names: 162148937

Gene position: 3254984-3256333 (Clockwise)

Preceding gene: 162148936

Following gene: 162148939

Centisome position: 82.53

GC content: 70.74

Gene sequence:

>1350_bases
ATGACATCGCCCCCGTCCGCCCCACCCCGCCGCCGCGCCCTGCGCGGACCGGCCGTCACCTTCCGTGCCGACCCGTTCCT
GGACGATCCGTCCCGGGCCCTGGTCCATGAGCCCGACGCGCTGATCCTGGTCGAGGACGGGCACATCACCCGTTTCGGCG
CCTTCGACGCGCTGCGCGATGCCATTCCCGAAGGCACGCCGGTCACGTCCTATCCCGACTGCCTCCTGTCGGCCGGGTTC
GTGGACACGCATGTCCATTACCCGCAACTGCCGATGATCGCGGCCTATGGCGAGCAGTTGCTGGAATGGCTGGAACGCTA
CACCTTCCCGACCGAGGCCCGCTTCGCCGATCCCGTCCATGCCGGCCAGGTCGCCCGGCGCTTCCTGCGCGAACTGCTGC
GGGCCGGCACCACCAGCGCCGCCGTGTACTGCACCGTCCATCCGCAGTCGGTCGAGGCGTTCTTCGCCGAATCGGCCCGG
CTGAACACCCGCATGGTGGCGGGCAAGGTCCTGATGGACCGCAACGCCCCCGACGGCCTGCGCGACACCCCCCAGGGCGG
ATATGACGAATCGCTGGCCCTGATCGACCGCTGGCACGGGCGCGGACGCCAGTTGTACGCCGTGACCCCGCGCTTCGCCG
CCACCAGCACCGAGGAACAGCTCGACCTGGCCGGCAGCCTGCTGCGCCAGCGCGACGGGCTGTTCATGCAGACCCACCTG
TCGGAAAACCAGGCGGAGGTCGACTGGATCCGCCAGCTCTTCCCCCGGCGCCGGTCCTATCTGGATGTCTATGCCCATGC
GGGGCTGGTCGGGCCGCGCAGCGTGCTGGGCCACGGCATCCATGTGGACGAGGAGAATTTCTGCACCTGCCACGCCGCCG
GCTGCGCGCTGGCGCACTGCCCGACATCGAACCTGTTCCTGGGCAGCGGCGCGTTCCGCCTGTTCGACGCGGTGGACCCG
CGCCGTCCGGTCCGCGTCGGGCTGGGCACCGATATCGGTGCCGGAACCAGCCTGTCGCAGCTCCAGACGCTGAACGAGGC
TTACAAGGTCGCGCAATCGACCGGCCGGCGGCTGCACCCGGCGCAGGCCTTCTGGCTGGCCACCGCCGGCGGCGCGCGAT
CGCTGTACCTGGACGACCGGATCGGCACGATCGCCCCGGGCATGGAGGCCGACCTGTGCGTCCTGGACCCCCGCGCCACC
CCGCTGATGGCCCAGCGCGCCGAACGGTGCGAGACGATCGAGGACCTGCTGTTCGTCCTGATGATGCTGGGCGACGACCG
CAGCGTCCGCGCAACCTACGTGGCCGGCGAACTGCTGCACGACCGCGACGCGGCCCCGCAGGCGGCATAG

Upstream 100 bases:

>100_bases
GCGTCGAAACCCTATCGAATCGCCCAGTCCTGACCGGCTTCCAATGGCCGGACGAAACAGGCAAGACTGACCGGCAATTC
CCGAGTGCCGAGCCCCGATC

Downstream 100 bases:

>100_bases
GCCGGACCTGCGCCCCGCGCCGTCAGGGACCGGTCGTGGCGTGATGGTCCGCCGGGTCCGCCTGTCCATGCGACGGCGGA
CCGGAAGCAGCTGAAAAAAG

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTSPPSAPPRRRALRGPAVTFRADPFLDDPSRALVHEPDALILVEDGHITRFGAFDALRDAIPEGTPVTSYPDCLLSAGF
VDTHVHYPQLPMIAAYGEQLLEWLERYTFPTEARFADPVHAGQVARRFLRELLRAGTTSAAVYCTVHPQSVEAFFAESAR
LNTRMVAGKVLMDRNAPDGLRDTPQGGYDESLALIDRWHGRGRQLYAVTPRFAATSTEEQLDLAGSLLRQRDGLFMQTHL
SENQAEVDWIRQLFPRRRSYLDVYAHAGLVGPRSVLGHGIHVDEENFCTCHAAGCALAHCPTSNLFLGSGAFRLFDAVDP
RRPVRVGLGTDIGAGTSLSQLQTLNEAYKVAQSTGRRLHPAQAFWLATAGGARSLYLDDRIGTIAPGMEADLCVLDPRAT
PLMAQRAERCETIEDLLFVLMMLGDDRSVRATYVAGELLHDRDAAPQAA

Sequences:

>Translated_449_residues
MTSPPSAPPRRRALRGPAVTFRADPFLDDPSRALVHEPDALILVEDGHITRFGAFDALRDAIPEGTPVTSYPDCLLSAGF
VDTHVHYPQLPMIAAYGEQLLEWLERYTFPTEARFADPVHAGQVARRFLRELLRAGTTSAAVYCTVHPQSVEAFFAESAR
LNTRMVAGKVLMDRNAPDGLRDTPQGGYDESLALIDRWHGRGRQLYAVTPRFAATSTEEQLDLAGSLLRQRDGLFMQTHL
SENQAEVDWIRQLFPRRRSYLDVYAHAGLVGPRSVLGHGIHVDEENFCTCHAAGCALAHCPTSNLFLGSGAFRLFDAVDP
RRPVRVGLGTDIGAGTSLSQLQTLNEAYKVAQSTGRRLHPAQAFWLATAGGARSLYLDDRIGTIAPGMEADLCVLDPRAT
PLMAQRAERCETIEDLLFVLMMLGDDRSVRATYVAGELLHDRDAAPQAA
>Mature_448_residues
TSPPSAPPRRRALRGPAVTFRADPFLDDPSRALVHEPDALILVEDGHITRFGAFDALRDAIPEGTPVTSYPDCLLSAGFV
DTHVHYPQLPMIAAYGEQLLEWLERYTFPTEARFADPVHAGQVARRFLRELLRAGTTSAAVYCTVHPQSVEAFFAESARL
NTRMVAGKVLMDRNAPDGLRDTPQGGYDESLALIDRWHGRGRQLYAVTPRFAATSTEEQLDLAGSLLRQRDGLFMQTHLS
ENQAEVDWIRQLFPRRRSYLDVYAHAGLVGPRSVLGHGIHVDEENFCTCHAAGCALAHCPTSNLFLGSGAFRLFDAVDPR
RPVRVGLGTDIGAGTSLSQLQTLNEAYKVAQSTGRRLHPAQAFWLATAGGARSLYLDDRIGTIAPGMEADLCVLDPRATP
LMAQRAERCETIEDLLFVLMMLGDDRSVRATYVAGELLHDRDAAPQAA

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=375, Percent_Identity=32.2666666666667, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1789249, Length=435, Percent_Identity=48.0459770114943, Blast_Score=420, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319963, Length=400, Percent_Identity=32, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24643849, Length=390, Percent_Identity=32.8205128205128, Blast_Score=194, Evalue=8e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 49341; Mature: 49210

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPPSAPPRRRALRGPAVTFRADPFLDDPSRALVHEPDALILVEDGHITRFGAFDALRD
CCCCCCCCHHHHHCCCCEEEEECCCCCCCCCHHHEECCCEEEEEECCCEEEEHHHHHHHH
AIPEGTPVTSYPDCLLSAGFVDTHVHYPQLPMIAAYGEQLLEWLERYTFPTEARFADPVH
HCCCCCCCCCCCHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
AGQVARRFLRELLRAGTTSAAVYCTVHPQSVEAFFAESARLNTRMVAGKVLMDRNAPDGL
HHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHEECCCCCCCC
RDTPQGGYDESLALIDRWHGRGRQLYAVTPRFAATSTEEQLDLAGSLLRQRDGLFMQTHL
CCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
SENQAEVDWIRQLFPRRRSYLDVYAHAGLVGPRSVLGHGIHVDEENFCTCHAAGCALAHC
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCEEEECCCEEEEEC
PTSNLFLGSGAFRLFDAVDPRRPVRVGLGTDIGAGTSLSQLQTLNEAYKVAQSTGRRLHP
CCCCEEECCCHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
AQAFWLATAGGARSLYLDDRIGTIAPGMEADLCVLDPRATPLMAQRAERCETIEDLLFVL
CCEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
MMLGDDRSVRATYVAGELLHDRDAAPQAA
HHHCCCCCEEEEEHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSPPSAPPRRRALRGPAVTFRADPFLDDPSRALVHEPDALILVEDGHITRFGAFDALRD
CCCCCCCHHHHHCCCCEEEEECCCCCCCCCHHHEECCCEEEEEECCCEEEEHHHHHHHH
AIPEGTPVTSYPDCLLSAGFVDTHVHYPQLPMIAAYGEQLLEWLERYTFPTEARFADPVH
HCCCCCCCCCCCHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
AGQVARRFLRELLRAGTTSAAVYCTVHPQSVEAFFAESARLNTRMVAGKVLMDRNAPDGL
HHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHEECCCCCCCC
RDTPQGGYDESLALIDRWHGRGRQLYAVTPRFAATSTEEQLDLAGSLLRQRDGLFMQTHL
CCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
SENQAEVDWIRQLFPRRRSYLDVYAHAGLVGPRSVLGHGIHVDEENFCTCHAAGCALAHC
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCEEEECCCEEEEEC
PTSNLFLGSGAFRLFDAVDPRRPVRVGLGTDIGAGTSLSQLQTLNEAYKVAQSTGRRLHP
CCCCEEECCCHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
AQAFWLATAGGARSLYLDDRIGTIAPGMEADLCVLDPRATPLMAQRAERCETIEDLLFVL
CCEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
MMLGDDRSVRATYVAGELLHDRDAAPQAA
HHHCCCCCEEEEEHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]