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The map label for this gene is yfcG [H]

Identifier: 162148934

GI number: 162148934

Start: 3250565

End: 3251320

Strand: Direct

Name: yfcG [H]

Synonym: GDI_3164

Alternate gene names: 162148934

Gene position: 3250565-3251320 (Clockwise)

Preceding gene: 162148933

Following gene: 162148935

Centisome position: 82.41

GC content: 66.8

Gene sequence:

>756_bases
GTGCGGTATGATCACCCTGCCGGCCGTCCCATCGGCCGGCGATCGGGAGGCACCATGATCGACCTGTATTACTGGACGAC
GCCGAACGGCCACAAGATCACGCTGTTCCTGGAAGAAACCGGGCTGCCCTATCGCCTGATCCCGATCAATATCGGCAAGG
GCGAACAATTCGCCCCCGACTTCCTGAAGATCGCGCCGAACAACCGCATCCCCGCCATCGTCGATCAGGACCCGGTCGTG
GGCGGGCCGCCGATCCCGCTCTTCGAATCCGGGGCGATCCTCTATTACCTGGCCCGCAAGACAGGCCGTTTCCTGCCCGC
CACGCTGCGGGAGGAAACCGAGGTCATGACATGGCTGTTCTGGCAGATGGGCGGCCTGGGCCCCATGGCCGGGCAGAACC
ATCATTTCCGCCTCTACGCCCCCGAACGCCTGCCCTATGCAACCACGCGCTATACCCAGGAAACCGAGCGCCTGTACGGC
GTGCTGGACCGCCACCTGGCGGGCCGGGACTTCATCGCGGGCGGCGGATACAGCATCGCCGACATGGCCTGCCACCCCTG
GATCATCCCCGAAAACCAGGGACAGGACATGGCCGCCTTCCCCAACCTGGCGCAATGGCATGCCCGGATCGCCGCACGAC
CGGCGACCCGACGGGCCTACAGCCTGACCGACCCCGTCCGCGCCGGGCAGCCGCCGGTGCGCGAACCGGCCGAACGCGCG
GCGCTCTATGCCACGCTCGACACAACCGCCGATTGA

Upstream 100 bases:

>100_bases
AGGGCGCGGCGGACGCCGCCCCGACCCGCGAGGAAGTGGAGGGCCTGCTTTCGGCACAGCCGCGATAAGTATCCGACCTT
TTGGCGCGACCCCGCGTTGC

Downstream 100 bases:

>100_bases
ACGTCTGCATCATGCCGCACGCGCACGCCAGAGTGACGCTGGCCCATGAGGTCGATTTCGCGGGCTGGCGAACCGCGACG
CGCCGCCTGGTCATGGCCGG

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRYDHPAGRPIGRRSGGTMIDLYYWTTPNGHKITLFLEETGLPYRLIPINIGKGEQFAPDFLKIAPNNRIPAIVDQDPVV
GGPPIPLFESGAILYYLARKTGRFLPATLREETEVMTWLFWQMGGLGPMAGQNHHFRLYAPERLPYATTRYTQETERLYG
VLDRHLAGRDFIAGGGYSIADMACHPWIIPENQGQDMAAFPNLAQWHARIAARPATRRAYSLTDPVRAGQPPVREPAERA
ALYATLDTTAD

Sequences:

>Translated_251_residues
MRYDHPAGRPIGRRSGGTMIDLYYWTTPNGHKITLFLEETGLPYRLIPINIGKGEQFAPDFLKIAPNNRIPAIVDQDPVV
GGPPIPLFESGAILYYLARKTGRFLPATLREETEVMTWLFWQMGGLGPMAGQNHHFRLYAPERLPYATTRYTQETERLYG
VLDRHLAGRDFIAGGGYSIADMACHPWIIPENQGQDMAAFPNLAQWHARIAARPATRRAYSLTDPVRAGQPPVREPAERA
ALYATLDTTAD
>Mature_251_residues
MRYDHPAGRPIGRRSGGTMIDLYYWTTPNGHKITLFLEETGLPYRLIPINIGKGEQFAPDFLKIAPNNRIPAIVDQDPVV
GGPPIPLFESGAILYYLARKTGRFLPATLREETEVMTWLFWQMGGLGPMAGQNHHFRLYAPERLPYATTRYTQETERLYG
VLDRHLAGRDFIAGGGYSIADMACHPWIIPENQGQDMAAFPNLAQWHARIAARPATRRAYSLTDPVRAGQPPVREPAERA
ALYATLDTTAD

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=202, Percent_Identity=58.4158415841584, Blast_Score=243, Evalue=9e-66,
Organism=Escherichia coli, GI87082195, Length=229, Percent_Identity=39.7379912663755, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI87081789, Length=204, Percent_Identity=28.921568627451, Blast_Score=61, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324100, Length=234, Percent_Identity=30.7692307692308, Blast_Score=112, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28571670, Length=174, Percent_Identity=27.5862068965517, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17864594, Length=197, Percent_Identity=26.3959390862944, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864592, Length=195, Percent_Identity=25.6410256410256, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 28132; Mature: 28132

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYDHPAGRPIGRRSGGTMIDLYYWTTPNGHKITLFLEETGLPYRLIPINIGKGEQFAPD
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCC
FLKIAPNNRIPAIVDQDPVVGGPPIPLFESGAILYYLARKTGRFLPATLREETEVMTWLF
CEEECCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
WQMGGLGPMAGQNHHFRLYAPERLPYATTRYTQETERLYGVLDRHLAGRDFIAGGGYSIA
HHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHH
DMACHPWIIPENQGQDMAAFPNLAQWHARIAARPATRRAYSLTDPVRAGQPPVREPAERA
HHCCCCEECCCCCCCCHHHCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHE
ALYATLDTTAD
EEEEEECCCCC
>Mature Secondary Structure
MRYDHPAGRPIGRRSGGTMIDLYYWTTPNGHKITLFLEETGLPYRLIPINIGKGEQFAPD
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCC
FLKIAPNNRIPAIVDQDPVVGGPPIPLFESGAILYYLARKTGRFLPATLREETEVMTWLF
CEEECCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
WQMGGLGPMAGQNHHFRLYAPERLPYATTRYTQETERLYGVLDRHLAGRDFIAGGGYSIA
HHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHH
DMACHPWIIPENQGQDMAAFPNLAQWHARIAARPATRRAYSLTDPVRAGQPPVREPAERA
HHCCCCEECCCCCCCCHHHCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHE
ALYATLDTTAD
EEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]