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The map label for this gene is rsmB [H]

Identifier: 161870942

GI number: 161870942

Start: 2080472

End: 2081731

Strand: Reverse

Name: rsmB [H]

Synonym: NMCC_2036

Alternate gene names: 161870942

Gene position: 2081731-2080472 (Counterclockwise)

Preceding gene: 161870943

Following gene: 161870941

Centisome position: 96.67

GC content: 58.57

Gene sequence:

>1260_bases
ATGAGTATGTCCCTCGCCCAAAAACTGGCCGCCGACAGCGTTGCCGCGGTTGCCGAAGGGCGCAATCTTCAGGATGTTTT
GGCGGAAATCCGCGCGGCGCATCCGCAGCTGACGGCGCAGGAAAACGGCGCGTTGCAGGACATCGCCTACGGCTGCCAGC
GTTATTTGGGCAGTTTGAAACATATGCTCGCGCAGATGCTGAAAAAGCCGATTGACAACCCGCAGCTCGAAAGCCTGCTT
TTGGCGGCGTTGTACCAACTGCATTACACGCGCAACGCGCCGCACGCCGTGGTCAATGAGGCGGTGGAAAGCATCGCGAA
AATCGGGCGCGGGCAGTACCGTTCGTTTGCCAACGCGGTTTTGCGCCGCTTTTTGCGCGAACGCGACAAGCTCGCGGCTT
CCTGCAAAAAAGATGATGTGGCGAAACACAACCTGCCGCTGTGGTGGGTGGCTTACTTGAAAAACCATTATCCGAAACAC
TGGCACAACATCACCGCCGCGCTGCAATCGCATCCGCCGATGACGTTGCGCGTCAACCGCCGGCACGGCAATGCCGAAAG
CTATTTGGAAAAACTGGCTGCGGAAGGTATCGCCGCAAAGGCGTTGGACGAATATGCGGTTACGTTGGAAGAAGCCGTGC
CGGTGAACCGCCTGCCCGGTTTTTCAGACGGCATTGTTTCGGTACAGGACTTCGGCGCGCAGCAGGCGGCGTATTTGTTA
AACCCGAAAGACGGCGAACGGATTTTGGACGCGTGCGCCGCGCCGGGCGGCAAGACGGGGCATATCTTGGAACTGGCGGA
TTGCCGCGTTACCGCCTTGGACATTGATGCAGGCCGTCTGAAGCGTGTGGAGGACAATATCGCCCGTTTGGGCTTTCAGA
CGGCATCGGCGGCGTGTGCCGATGCGCGGGATTTGGCGGCGTGGTATGATGGAAAGCCTTTCGACGCAATCCTTGCCGAC
GTGCCATGCACCGCTTCCGGCGTGGCGCGGCGCAATCCCGACGTGAAATGGCTGCGCCGTCCGACCGACGCGCTCAAAAC
CGCCCGCCAGCAGGAAGCCCTGCTGGACGCACTTTGGCAGACGCTGAAACAGGGCGGCAGAATGCTGCTTGCCACCTGTT
CCGTGTTCGTCGAAGAAAACGACGGGCAATTGCAAAAATTCCTCAACCGCCATGCCGATGCCGAACCGATCGAATCGCGG
GTGCTTTTACCGAACAAACACCAAGATGGTTTTTATTACGCGCTTATTCAAAAGCATTAA

Upstream 100 bases:

>100_bases
AAAACAGATGCCGAATATTATCGGGCATTGCTGGCAGCCGAACAGGCCGTCTGAAATCCGCCGAACACTTGAAATCAAAT
ATGAATCAGAAAGCAAAAAT

Downstream 100 bases:

>100_bases
ACAATGGCTTGTGCTGCTGCCGATGCTTTCCGTTTTGCCGGACGCGGCGGCGGAGGGCATTGCCGCGACCCGCGCCGAAG
CGAGGATAACCGACGGCGGG

Product: 16S RNA methyltransferase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSMSLAQKLAADSVAAVAEGRNLQDVLAEIRAAHPQLTAQENGALQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLL
LAALYQLHYTRNAPHAVVNEAVESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRLPGFSDGIVSVQDFGAQQAAYLL
NPKDGERILDACAAPGGKTGHILELADCRVTALDIDAGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILAD
VPCTASGVARRNPDVKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHADAEPIESR
VLLPNKHQDGFYYALIQKH

Sequences:

>Translated_419_residues
MSMSLAQKLAADSVAAVAEGRNLQDVLAEIRAAHPQLTAQENGALQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLL
LAALYQLHYTRNAPHAVVNEAVESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRLPGFSDGIVSVQDFGAQQAAYLL
NPKDGERILDACAAPGGKTGHILELADCRVTALDIDAGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILAD
VPCTASGVARRNPDVKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHADAEPIESR
VLLPNKHQDGFYYALIQKH
>Mature_418_residues
SMSLAQKLAADSVAAVAEGRNLQDVLAEIRAAHPQLTAQENGALQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLL
AALYQLHYTRNAPHAVVNEAVESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKHW
HNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRLPGFSDGIVSVQDFGAQQAAYLLN
PKDGERILDACAAPGGKTGHILELADCRVTALDIDAGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADV
PCTASGVARRNPDVKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHADAEPIESRV
LLPNKHQDGFYYALIQKH

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=280, Percent_Identity=30, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI76150623, Length=280, Percent_Identity=30, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI32698918, Length=187, Percent_Identity=32.0855614973262, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI270288816, Length=185, Percent_Identity=31.3513513513514, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI23199998, Length=185, Percent_Identity=31.3513513513514, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI8922322, Length=185, Percent_Identity=31.3513513513514, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI270288818, Length=185, Percent_Identity=31.3513513513514, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI301336155, Length=252, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI39995082, Length=188, Percent_Identity=30.3191489361702, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI2367212, Length=425, Percent_Identity=41.6470588235294, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI87081985, Length=176, Percent_Identity=30.1136363636364, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536757, Length=280, Percent_Identity=27.5, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25143471, Length=307, Percent_Identity=27.3615635179153, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115534021, Length=169, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71998419, Length=169, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=238, Percent_Identity=28.9915966386555, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI22024126, Length=219, Percent_Identity=30.1369863013699, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24668781, Length=197, Percent_Identity=33.502538071066, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356513, Length=197, Percent_Identity=33.502538071066, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21355201, Length=199, Percent_Identity=28.1407035175879, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 46330; Mature: 46199

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMSLAQKLAADSVAAVAEGRNLQDVLAEIRAAHPQLTAQENGALQDIAYGCQRYLGSLK
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAVESIAKIGRGQYRSFANAV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKHWHNITAALQSHPPMTLRVNR
HHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEC
RHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRLPGFSDGIVSVQDFGAQQAAYLL
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHEEEC
NPKDGERILDACAAPGGKTGHILELADCRVTALDIDAGRLKRVEDNIARLGFQTASAACA
CCCCHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHH
DARDLAAWYDGKPFDAILADVPCTASGVARRNPDVKWLRRPTDALKTARQQEALLDALWQ
HHHHHHHHCCCCCHHHHHHCCCCCCCCCHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
TLKQGGRMLLATCSVFVEENDGQLQKFLNRHADAEPIESRVLLPNKHQDGFYYALIQKH
HHHHCCCEEEEHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
SMSLAQKLAADSVAAVAEGRNLQDVLAEIRAAHPQLTAQENGALQDIAYGCQRYLGSLK
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAVESIAKIGRGQYRSFANAV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKHWHNITAALQSHPPMTLRVNR
HHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEC
RHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRLPGFSDGIVSVQDFGAQQAAYLL
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHEEEC
NPKDGERILDACAAPGGKTGHILELADCRVTALDIDAGRLKRVEDNIARLGFQTASAACA
CCCCHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHH
DARDLAAWYDGKPFDAILADVPCTASGVARRNPDVKWLRRPTDALKTARQQEALLDALWQ
HHHHHHHHCCCCCHHHHHHCCCCCCCCCHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
TLKQGGRMLLATCSVFVEENDGQLQKFLNRHADAEPIESRVLLPNKHQDGFYYALIQKH
HHHHCCCEEEEHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA