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The map label for this gene is gidA [H]

Identifier: 161870869

GI number: 161870869

Start: 2008880

End: 2010808

Strand: Direct

Name: gidA [H]

Synonym: NMCC_1957

Alternate gene names: 161870869

Gene position: 2008880-2010808 (Clockwise)

Preceding gene: 161870868

Following gene: 161870870

Centisome position: 93.29

GC content: 57.54

Gene sequence:

>1929_bases
ATGCCGTCTGAAACACCGACAGGCAATACACACATGACCCACATGATTTACCCCAAAACCTACGACGTTATCGTCGTCGG
CGGCGGACACGCCGGCACAGAAGCCGCGCTCGCCGCCGCCCGTATGGGCGCGCAGACGCTTTTGCTCACACACAATATTG
AAACGCTCGGACAAATGTCGTGCAACCCTTCCATCGGCGGCATCGGCAAAGGGCATTTGGTGCGCGAACTCGACGCGCTC
GGCGGCGCGATGGCGTTGGCAACCGACAAATCCGGCATCCAGTTCCGCCGTCTGAACGCCAGCAAAGGCGCGGCAGTACG
GGCCACGCGCGCGCAGGCGGATCGTATCCTATATAAAGCCGCCATCCGCGAAATGTTGGAAAACCAAGAAAACCTCGACC
TTTTCCAACAAGCCGTCGAAGACGTAACGCTCGATGGCGACCGCATTTCAGGCGTAATCACCGCGATGGGCGTGGAGTTT
AAAGCACGCGCCGTGGTGTTGACCGCAGGCACGTTTTTGTCCGGCAAAATCCACATCGGTTTGGAAAACTACGAGGGCGG
ACGCGCCGGCGACCCCGCCGCCAAATCGCTGGGCGGACGTTTGCGCGAATTGAAGCTGCCGCAAGGCCGTCTGAAAACCG
GCACGCCGCCGCGCATCGACGGACGCACGATTGACTTTTCCCAACTGACCGAACAGCCCGGCGACACACCCGTTCCCGTC
ATGTCCGTGCGCGGCAACGCCGAAATGCACCCGCGCCAAGTGTCCTGCTGGATTACGCATACCAACACGCAAACCCACGA
CATCATCCGCTCAGGCTTCGACCGCAGCCCCATGTTCACCGGCAAAATCGAAGGTGTCGGCCCACGCTACTGTCCGTCTA
TCGAAGACAAAATCAACCGCTTCGCCGACAAAGACAGCCACCAGATTTTCCTCGAACCCGAAGGTCTGACCACGCATGAA
TACTATCCAAACGGTATCTCCACCAGCCTGCCGTTCGACATCCAAATCGCCCTCGTGCGCAGCATGAAAGGCCTCGAAAA
CGCCCATATCCTGCGCCCCGGCTACGCCATCGAATACGACTACTTCGACCCGCGCAACCTCAAAGCCAGCCTTGAAACCA
AAACCATCCAAGGCCTCTTTTTCGCCGGACAAATCAACGGCACAACGGGCTACGAAGAAGCCGCCGCGCAAGGCTTGCTC
GCAGGCGCAAACGCCGTGCAATACGTCCGCGAACAAGACCCGCTCCTGCTGCGCCGCGAACAAGCCTATCTCGGCGTATT
GGTGGACGACCTCATCACCAAAGGCGTGAACGAGCCTTACCGCATGTTCACCAGCCGCGCCGAATACCGCCTGCAACTCA
GGGAAGACAACGCCGACATGCGTCTGACCGAAGACGGCTACAAAATCGGCTTGGTGGGTGAAGAACAATGGCGTATGTTC
AACGAAAAACGTGAAGCCATCGAACGTGAAATCCAACGTTTGAAAACAACGTGGTACACACCGCAAAAGCTCGCCGAAGA
CGAACAAATCCGCGTGTTCGGACAAAAACTCAGCCGCGAAGCCAACCTGCACGACCTCCTGCGCCGCCCGAACCTCGATT
ACGCCGCGCTGATGACGCTCGAAGGCGCGATGCCGTCTGAAAACCTCTCCGCCGAAGTCATCGAGCAAGTCGAAATCCAA
GTCAAATACCAAGGCTATATCGACCGCCAAAACGAAGAAATCGACAGCCGCCGCGACATCGAAACCTTAAAACTGCCCGA
CGACATCGATTACAGCAAAGTCAAAGGCTTATCGGCAGAAGTACAGCAAAAGCTCAACCAGCACAAACCCGAAACCGTCG
GACAAGCAAGCCGCATTTCCGGCGTAACCCCTGCGGCAGTGGCATTGCTGATGGTGCATTTGAAGCGCGGGTTTAAAGAC
GCGAAATAA

Upstream 100 bases:

>100_bases
GTACGAAACCGATTCACTTGGTACTTCAGCACCTTAGAGAATCGTTCTCTTTGAGCTAAGGCGAGGCAACGCTGTACTGG
TTTTTGTTAATCCACTATAA

Downstream 100 bases:

>100_bases
ACACATCGGTACGATGCCGTCTGAAGCCCTTTCAGACGGCATTCCTACTATCCCAACAGGAAACATCATGCACATACTGA
CCGCCGGCGTGGACGAGGCA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MPSETPTGNTHMTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIETLGQMSCNPSIGGIGKGHLVRELDAL
GGAMALATDKSGIQFRRLNASKGAAVRATRAQADRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEF
KARAVVLTAGTFLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQLTEQPGDTPVPV
MSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVGPRYCPSIEDKINRFADKDSHQIFLEPEGLTTHE
YYPNGISTSLPFDIQIALVRSMKGLENAHILRPGYAIEYDYFDPRNLKASLETKTIQGLFFAGQINGTTGYEEAAAQGLL
AGANAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMRLTEDGYKIGLVGEEQWRMF
NEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSREANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQ
VKYQGYIDRQNEEIDSRRDIETLKLPDDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLKRGFKD
AK

Sequences:

>Translated_642_residues
MPSETPTGNTHMTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIETLGQMSCNPSIGGIGKGHLVRELDAL
GGAMALATDKSGIQFRRLNASKGAAVRATRAQADRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEF
KARAVVLTAGTFLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQLTEQPGDTPVPV
MSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVGPRYCPSIEDKINRFADKDSHQIFLEPEGLTTHE
YYPNGISTSLPFDIQIALVRSMKGLENAHILRPGYAIEYDYFDPRNLKASLETKTIQGLFFAGQINGTTGYEEAAAQGLL
AGANAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMRLTEDGYKIGLVGEEQWRMF
NEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSREANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQ
VKYQGYIDRQNEEIDSRRDIETLKLPDDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLKRGFKD
AK
>Mature_641_residues
PSETPTGNTHMTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIETLGQMSCNPSIGGIGKGHLVRELDALG
GAMALATDKSGIQFRRLNASKGAAVRATRAQADRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFK
ARAVVLTAGTFLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQLTEQPGDTPVPVM
SVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVGPRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEY
YPNGISTSLPFDIQIALVRSMKGLENAHILRPGYAIEYDYFDPRNLKASLETKTIQGLFFAGQINGTTGYEEAAAQGLLA
GANAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMRLTEDGYKIGLVGEEQWRMFN
EKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSREANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQV
KYQGYIDRQNEEIDSRRDIETLKLPDDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLKRGFKDA
K

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=634, Percent_Identity=43.8485804416404, Blast_Score=485, Evalue=1e-137,
Organism=Homo sapiens, GI19882217, Length=659, Percent_Identity=42.185128983308, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI183227703, Length=674, Percent_Identity=41.2462908011869, Blast_Score=466, Evalue=1e-131,
Organism=Escherichia coli, GI2367273, Length=622, Percent_Identity=67.524115755627, Blast_Score=852, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=640, Percent_Identity=39.375, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6321202, Length=619, Percent_Identity=44.4264943457189, Blast_Score=503, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24658174, Length=638, Percent_Identity=46.8652037617555, Blast_Score=512, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 71293; Mature: 71162

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSETPTGNTHMTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIETLGQMS
CCCCCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECHHHHHCCC
CNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQADRILYKA
CCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCEEEEHHHHHHHHHHHH
AIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGTFLSGKIHIG
HHHHHHCCCCCHHHHHHHHHHEECCCHHHHHHHEEECCCEEEEEEEEEECCEEECEEEEE
LENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQLTEQPGDTPVPV
EECCCCCCCCCHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEECHHHHHCCCCCCCCCE
MSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVGPRYCPSIEDKINR
EEECCCCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHH
FADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGLENAHILRPGYAIEYD
HHCCCCCEEEECCCCCEECCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEEC
YFDPRNLKASLETKTIQGLFFAGQINGTTGYEEAAAQGLLAGANAVQYVREQDPLLLRRE
CCCCCCCEEECCCEEEEEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCEEEEH
QAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMRLTEDGYKIGLVGEEQWRMF
HHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEECCCCCEEEECCCEEEEEECHHHHHHH
NEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSREANLHDLLRRPNLDYAALMTL
HHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEE
EGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSRRDIETLKLPDDIDYSKVKGLSAE
CCCCCCCCCCHHHHHEEEEEEEEECEECCCCCCHHHHCCCEEEECCCCCCHHHHCCCHHH
VQQKLNQHKPETVGQASRISGVTPAAVALLMVHLKRGFKDAK
HHHHHHHCCCHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PSETPTGNTHMTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIETLGQMS
CCCCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECHHHHHCCC
CNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQADRILYKA
CCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCEEEEHHHHHHHHHHHH
AIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGTFLSGKIHIG
HHHHHHCCCCCHHHHHHHHHHEECCCHHHHHHHEEECCCEEEEEEEEEECCEEECEEEEE
LENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQLTEQPGDTPVPV
EECCCCCCCCCHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEECHHHHHCCCCCCCCCE
MSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVGPRYCPSIEDKINR
EEECCCCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHH
FADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGLENAHILRPGYAIEYD
HHCCCCCEEEECCCCCEECCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEEC
YFDPRNLKASLETKTIQGLFFAGQINGTTGYEEAAAQGLLAGANAVQYVREQDPLLLRRE
CCCCCCCEEECCCEEEEEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCEEEEH
QAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMRLTEDGYKIGLVGEEQWRMF
HHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEECCCCCEEEECCCEEEEEECHHHHHHH
NEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSREANLHDLLRRPNLDYAALMTL
HHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEE
EGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSRRDIETLKLPDDIDYSKVKGLSAE
CCCCCCCCCCHHHHHEEEEEEEEECEECCCCCCHHHHCCCEEEECCCCCCHHHHCCCHHH
VQQKLNQHKPETVGQASRISGVTPAAVALLMVHLKRGFKDAK
HHHHHHHCCCHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA