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The map label for this gene is murE [H]

Identifier: 161870660

GI number: 161870660

Start: 1775138

End: 1776616

Strand: Reverse

Name: murE [H]

Synonym: NMCC_1730

Alternate gene names: 161870660

Gene position: 1776616-1775138 (Counterclockwise)

Preceding gene: 161870661

Following gene: 161870659

Centisome position: 82.5

GC content: 57.13

Gene sequence:

>1479_bases
ATGTTCAGCAAGTTAACCCCTTTGGCTGAAACCGGCATCCCGACCCTGTCGTGTGCAAACGCGGCAGGGCGTTTGTTGCA
TTCAGACAGCCGCCAAATCAAACAAGGCGATATTTTCGTTGCCTGTCCGGGCGAATATGCCGACGGACGCAGTTATATCC
CCGCCGCCGTTGCCAACGGCGCGGCTTTTGTTTTTTGGGACGACGATGGCAAATTCGCGTGGAATCCCGAATGGAAAGTC
CCCAATCAAGGCATCAAAGATTTGAAACACCGTGCCGGCATATTGGCGGCGCAAGTTTACGGCAACGTTTCAGACGGTCT
CAAAGTCTGGGGCGTAACCGGAACCAACGGCAAAACCTCCATCACGCAATGGCTGGCGCAAGCTGCCGATTTGTTGGGCG
AAAAAACCGCCATTGTCGGCACGGTCGGCAACGGCTTTTGGGGTGCATTGGAAGAAACCACGCATACCACACCCGCCCCC
GTCGATGTCCAAACCCTGCTCTACCGTTTCCGTCAACAAGGCGCAACAGTCGCCGCGATGGAAGTCTCCAGCCACGGGCT
TGACCAGTCGCGCGTCAACGGCGTGTCATTCCGCAGCGCAATCTTTACCAACCTCACCCGCGACCACCTCGACTACCACG
GCACGATGGAAGCCTACGGTGCCATCAAGTCGCGCCTGTTTTACTGGCACGGCTTGAAACACGCAGTCATCAACGTGGAT
GACGAATACGGCGCGGAACTCGTAGGTCGTCTGAAAAAAGACTGTCCCGATTTGGCCGTTTATAGCTACGGCTTCAGCGA
ACACGCCGACATCCGCATTATCGATTTCACCGCCTCTTCAGACGGCATAGCAGCCGTATTCCAAACCCCGTGGGGCGAAG
GGAAATGCCGCACGCGCCTGCTCGGACGGTTCAACGCGCAAAACCTCGCCGCCTGCATCGCCTTGCTGTGCGCCAACGGC
TATCCGCTTGATAATGTATTGGATGTGCTGGCAAAAATCCGTCCCGCTTCAGGGCGCATGGACTGCATCATGAACAGCGG
CAAGCCCTTGGTCGTCGTCGATTACGCCCACACGCCCGACGCATTGGAAAAAGCACTCGCCACCTTGCAGGAAATCAAAC
CGCAGGGCGCGGCTTTGTGGTGCGTATTCGGTTGCGGCGGCAACCGCGATCGCGGCAAACGCCTGCTGATGGGCGCGGCA
GCCGTACAGGGCGCGGATAAAGTCGTCGTAACCAGCGACAATCCGCGTTTGGAAAATCCGCACGACATCATCAACGACAT
CCTGCCTGCCGTTCCCGCGCCCGAACGTGTCGAAGCCGACCGCGCCGCTGCTATCCGTTATGCAGTCGAACGCGCCGCCG
CAAACGACATCATCCTGATTGCCGGCAAAGGGCATGAAAACTATCAGGATGTACAAGGCGTGAAGCACCGTTTTTCCGAT
CTTGAAATCGTCGGACAGGCTTTGTTAACTCGTAAATAA

Upstream 100 bases:

>100_bases
GGGCGGCAGCCTGAACATCTTGGGCATTTCCCCGACCAAGCCACTGACCGCCGCAGCCGTCAAAACACCGTCTTAATCCG
AGTATCAACGAGATTGTTTT

Downstream 100 bases:

>100_bases
TGGGATATTCGGACGGCATCGTATGAAACAATCCGCCCGAATAAAAAATATGGATCAGACATTAAAAAATACATTGGGCA
TTTGCGCGCTTTTAGCCTTT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPGEYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKV
PNQGIKDLKHRAGILAAQVYGNVSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEAYGAIKSRLFYWHGLKHAVINVD
DEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDFTASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANG
YPLDNVLDVLAKIRPASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRGKRLLMGAA
AVQGADKVVVTSDNPRLENPHDIINDILPAVPAPERVEADRAAAIRYAVERAAANDIILIAGKGHENYQDVQGVKHRFSD
LEIVGQALLTRK

Sequences:

>Translated_492_residues
MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPGEYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKV
PNQGIKDLKHRAGILAAQVYGNVSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEAYGAIKSRLFYWHGLKHAVINVD
DEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDFTASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANG
YPLDNVLDVLAKIRPASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRGKRLLMGAA
AVQGADKVVVTSDNPRLENPHDIINDILPAVPAPERVEADRAAAIRYAVERAAANDIILIAGKGHENYQDVQGVKHRFSD
LEIVGQALLTRK
>Mature_492_residues
MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPGEYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKV
PNQGIKDLKHRAGILAAQVYGNVSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEAYGAIKSRLFYWHGLKHAVINVD
DEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDFTASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANG
YPLDNVLDVLAKIRPASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRGKRLLMGAA
AVQGADKVVVTSDNPRLENPHDIINDILPAVPAPERVEADRAAAIRYAVERAAANDIILIAGKGHENYQDVQGVKHRFSD
LEIVGQALLTRK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=480, Percent_Identity=45.625, Blast_Score=335, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53185; Mature: 53185

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPGEYADGRSYIPAAVANG
CCCCCCCHHHCCCCCEEHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCC
AAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGNVSDGLKVWGVTGTNGKTS
EEEEEECCCCCEEECCCCCCCCHHHHHHHHHHCEEEEEHHCCCCCCEEEEEEECCCCCHH
ITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAPVDVQTLLYRFRQQGATVAAM
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEE
EVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEAYGAIKSRLFYWHGLKHAVINVD
EHHHCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECC
DEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDFTASSDGIAAVFQTPWGEGKCRTRL
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHH
LGRFNAQNLAACIALLCANGYPLDNVLDVLAKIRPASGRMDCIMNSGKPLVVVDYAHTPD
HHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCHH
ALEKALATLQEIKPQGAALWCVFGCGGNRDRGKRLLMGAAAVQGADKVVVTSDNPRLENP
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEHHHHCCCCEEEEECCCCCCCCH
HDIINDILPAVPAPERVEADRAAAIRYAVERAAANDIILIAGKGHENYQDVQGVKHRFSD
HHHHHHHHCCCCCCHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
LEIVGQALLTRK
HHHHHHHHHCCC
>Mature Secondary Structure
MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPGEYADGRSYIPAAVANG
CCCCCCCHHHCCCCCEEHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCC
AAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGNVSDGLKVWGVTGTNGKTS
EEEEEECCCCCEEECCCCCCCCHHHHHHHHHHCEEEEEHHCCCCCCEEEEEEECCCCCHH
ITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAPVDVQTLLYRFRQQGATVAAM
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEE
EVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEAYGAIKSRLFYWHGLKHAVINVD
EHHHCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECC
DEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDFTASSDGIAAVFQTPWGEGKCRTRL
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHH
LGRFNAQNLAACIALLCANGYPLDNVLDVLAKIRPASGRMDCIMNSGKPLVVVDYAHTPD
HHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCHH
ALEKALATLQEIKPQGAALWCVFGCGGNRDRGKRLLMGAAAVQGADKVVVTSDNPRLENP
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEHHHHCCCCEEEEECCCCCCCCH
HDIINDILPAVPAPERVEADRAAAIRYAVERAAANDIILIAGKGHENYQDVQGVKHRFSD
HHHHHHHHCCCCCCHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
LEIVGQALLTRK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]