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The map label for this gene is prpF [H]

Identifier: 161870642

GI number: 161870642

Start: 1749028

End: 1750197

Strand: Reverse

Name: prpF [H]

Synonym: NMCC_1712

Alternate gene names: 161870642

Gene position: 1750197-1749028 (Counterclockwise)

Preceding gene: 161870643

Following gene: 161870641

Centisome position: 81.28

GC content: 56.92

Gene sequence:

>1170_bases
ATGCCGCAAATTAAAATTCCCGCCGTTTACTACCGTGGCGGTACATCAAAAGGCGTGTTTTTCAAACGTTCCGACCTGCC
CGAGGCGGCGCGGGAAGCGGGAAGCGCACGCGACAAAATCCTCTTGCGCGTACTCGGCAGCCCGGATCCCTACGGCAAGC
AGATAGACGGTTTGGGCAACGCCAGTTCGTCCACCAGCAAAGCCGTGATTTTGGACAAGTCCGAACGCGCCGATCACGAT
GTCGATTACCTTTTCGGGCAAGTTTCCATCGACAAACCTTTTGTCGATTGGAGCGGCAACTGCGGCAACCTCACCGCCGC
CGTGGGCGCATTCTCCATCGAACAGGGCTTGGTCGATAAAGGCAAGATTCCTTCAGACGGCATCTGCACGGTCAAAATCT
GGCAGAAAAACATCGGCAAAACCATTATTGCCCATGTACCGATGCAAAACGGCGCAGTTTTGGAAACAGGCGATTTTGAG
CTCGACGGCGTAACGTTCCCGGCAGCCGAAGTACAAATCGAATTTCTTGATTCAGCCGACGGCGAAGGCAGTATGTTCCC
AACCGGCAATTTGGTCGATGAAATTGATGTGCCGAATATAGGCCGTTTGAAAGCCACGCTCATCAACGCGGGCATTCCGA
CCGTTTTCCTGAATGCCGCCGACTTGGGCTACACGGGCAAAGAGTTGCAAGACGACATCAACAACGATGCCGCGGCTTTG
GAAAAATTCGAGAAAATCCGCGCTTACGGTGCGCTGAAAATGGGTCTGATCAGCGACGTATCCGAAGCTGCCGCCCGCGC
GCACACGCCGAAAGTCGCCTTCGTCGCGCCCGCCGCCGATTACACCGCCTCCAGTGGCAAAACCGTGAACGCCGCCGACA
TCGATTTGCTGGTACGCGCCCTGAGCATGGGCAAACTGCACCACGCGATGATGGGTACCGCCTCTGTTGCCATTGCGACC
GCCGCCGCCGTGCCCGGTACGCTGGTCAACCTTGCCGCAGGCGGCGGAACGCGTAAAGAAGTGCGCTTCGGGCATCCTTC
TGGCACATTGCGTGTCGGTGCAGCCGCCGAATGTCAGGACGGACAATGGACGACCACCAAAGCGGTTATGAGCCGCAGCG
CACGCGTGATGATGGAAGGTTGGGTCAGGGTGCCTGAGGATTGTTTTTAA

Upstream 100 bases:

>100_bases
CCCGACAGTTTGCGGAATCAAACGGCTTTGGTCGGAGTGGCAGCCTAATCCACTATAAAAATCGTGGGCAGAGCCCACGC
TACATAAGGAGAATCTAGAA

Downstream 100 bases:

>100_bases
ATCGACGTAGCGTGGGTTTGCTCGCGAGCCATAAAAAGGTCGTCTGAAAAACAAGTAAACATCAAATCACTGACCATTCC
TTTCCCTTGCCCTGTGGCGG

Product: putative AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRVLGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHD
VDYLFGQVSIDKPFVDWSGNCGNLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
LDGVTFPAAEVQIEFLDSADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLNAADLGYTGKELQDDINNDAAAL
EKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVAPAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIAT
AAAVPGTLVNLAAGGGTRKEVRFGHPSGTLRVGAAAECQDGQWTTTKAVMSRSARVMMEGWVRVPEDCF

Sequences:

>Translated_389_residues
MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRVLGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHD
VDYLFGQVSIDKPFVDWSGNCGNLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
LDGVTFPAAEVQIEFLDSADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLNAADLGYTGKELQDDINNDAAAL
EKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVAPAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIAT
AAAVPGTLVNLAAGGGTRKEVRFGHPSGTLRVGAAAECQDGQWTTTKAVMSRSARVMMEGWVRVPEDCF
>Mature_388_residues
PQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRVLGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDV
DYLFGQVSIDKPFVDWSGNCGNLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFEL
DGVTFPAAEVQIEFLDSADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLNAADLGYTGKELQDDINNDAAALE
KFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVAPAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATA
AAVPGTLVNLAAGGGTRKEVRFGHPSGTLRVGAAAECQDGQWTTTKAVMSRSARVMMEGWVRVPEDCF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=382, Percent_Identity=35.3403141361257, Blast_Score=185, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 40988; Mature: 40857

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRVLGSPDPYGKQIDGLGN
CCCEECCEEEEECCCCCCEEEECCCCCHHHHHCCCCHHEEEEEEECCCCCCCCCCCCCCC
ASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCGNLTAAVGAFSIEQGLVDK
CCCCCCEEEEEECCCCCCCCHHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFELDGVTFPAAEVQIEFLDSAD
CCCCCCCEEEEEEECCCCCCEEEEECCCCCCCEEECCCEEECCEECCHHEEEEEEEECCC
GEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLNAADLGYTGKELQDDINNDAAAL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHCCCHHHHH
EKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVAPAADYTASSGKTVNAADIDLLVRA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECHHHHHHHHHH
LSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAGGGTRKEVRFGHPSGTLRVGAAAECQD
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEECCCCCCCC
GQWTTTKAVMSRSARVMMEGWVRVPEDCF
CCEEHHHHHHHHHHHEEEHHCCCCCCCCC
>Mature Secondary Structure 
PQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRVLGSPDPYGKQIDGLGN
CCEECCEEEEECCCCCCEEEECCCCCHHHHHCCCCHHEEEEEEECCCCCCCCCCCCCCC
ASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCGNLTAAVGAFSIEQGLVDK
CCCCCCEEEEEECCCCCCCCHHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFELDGVTFPAAEVQIEFLDSAD
CCCCCCCEEEEEEECCCCCCEEEEECCCCCCCEEECCCEEECCEECCHHEEEEEEEECCC
GEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLNAADLGYTGKELQDDINNDAAAL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHCCCHHHHH
EKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVAPAADYTASSGKTVNAADIDLLVRA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECHHHHHHHHHH
LSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAGGGTRKEVRFGHPSGTLRVGAAAECQD
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEECCCCCCCC
GQWTTTKAVMSRSARVMMEGWVRVPEDCF
CCEEHHHHHHHHHHHEEEHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA