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The map label for this gene is ackA2 [H]

Identifier: 161870641

GI number: 161870641

Start: 1747415

End: 1748614

Strand: Reverse

Name: ackA2 [H]

Synonym: NMCC_1711

Alternate gene names: 161870641

Gene position: 1748614-1747415 (Counterclockwise)

Preceding gene: 161870642

Following gene: 161870640

Centisome position: 81.2

GC content: 56.17

Gene sequence:

>1200_bases
ATGTCCGACCAACTCATTCTTGTTCTGAACTGCGGCAGTTCATCGCTCAAAGGTGCCGTTATCGACCGCAAAAGCGGCAG
CGTCGTCCTAAGCTGCCTCGGCGAACGCCTGACCACGCCCGAAGCCGTCATTACCTTCAACAAAGACGGCAACAAACGCC
AAGTTCCCCTGAGCGGCCGCAACTGCCATGCCGGCGCGGTGGGTATGCTGTTGAACGAACTGGAAAAACACGGACTGCAC
GACCGCATCAAAGCCGTCGGCCACCGCATCGCCCACGGCGGCGAAAAATACAGCGAGTCTGTTTTGATCGACCAGGCCGT
AATGGACGAACTCAATGCCTGCATTCCGCTTGCGCCGCTGCACAACCCCGCCAACATCAGCGGCATCCTTGCCGCACAGG
AACATTTCCCCGGCCTGCCCAATGTCGGCGTGATGGATACTTCGTTCCACCAAACCATGCCGGAGCGTGCCTACACTTAT
GCCGTGCCGCGCGAGTTGCGTAAAAAATACGCTTTCCGCCGCTACGGTTTCCACGGCACCAGTATGCGTTACGTTGCCCC
TGAAGCCGCACGCATCTTGGGCAAACCTCTGGAAGACATCCGCATGATTATTGCCCACTTAGGCAACGGCGCATCCATTA
CCGCCATCAAAAACGGCAAATCCGTCGATACCAGTATGGGTTTCACGCCGATCGAAGGTTTGGTAATGGGTACACGTTGC
GGCGACATCGATCCGGGCGTATACAGCTATCTGACTTCCCACGCCGGGATGGATGTTGCCCAAGTGGATGAAATGCTGAA
CAAAAAATCAGGTTTGCTCGGTATTTCCGAACTTTCCAACGACTGCCGCACCCTCGAAATCGCCGCCGACGAAGGCCACG
AAGGCGCGCGCCTCGCCCTCGAAGTCATGACCTACCGCCTCGCCAAATACATCGCTTCGATGGCTGTGGCCTGCGGCGGC
GTTGACGCACTCGTGTTCACCGGCGGTATCGGCGAAAACTCGCGTAATATCCGTGCCAAAACCGTTTCCTATCTTGATTT
CTTGGGTCTGCACATCGACACCAAAGCCAATATGGAAAAACGCTACGGCAATTCGGGCATTATCAGCCCGACCGATTCTT
CTCCGGCTGTTTTGGTTGTCCCGACCAATGAAGAACTGATGATTGCCTGCGACACTGCCGAACTTGTCGGCATCTTGTAG

Upstream 100 bases:

>100_bases
TTGCCCAAAGAGGCCGTCTGACACACACTCCGCCCAACGCCCATCCTTTTTCAGACGACCCCACACCAAAAAACAACCAC
AAACTACAAGGAGAAACATC

Downstream 100 bases:

>100_bases
CCAAAAAAGGGACGAGTCCGCACAATGCCGTCTGAAACCCCAAACGCCCGATTAGGCTGATGAGGATTTTAGACGGCATT
GTTCATTTTTTTGTTATCTT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 2 [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVITFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLH
DRIKAVGHRIAHGGEKYSESVLIDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKNGKSVDTSMGFTPIEGLVMGTRC
GDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGISELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGG
VDALVFTGGIGENSRNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDTAELVGIL

Sequences:

>Translated_399_residues
MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVITFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLH
DRIKAVGHRIAHGGEKYSESVLIDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKNGKSVDTSMGFTPIEGLVMGTRC
GDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGISELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGG
VDALVFTGGIGENSRNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDTAELVGIL
>Mature_398_residues
SDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVITFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHD
RIKAVGHRIAHGGEKYSESVLIDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTYA
VPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKNGKSVDTSMGFTPIEGLVMGTRCG
DIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGISELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGV
DALVFTGGIGENSRNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDTAELVGIL

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=49.5, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI145693184, Length=391, Percent_Identity=45.7800511508951, Blast_Score=347, Evalue=8e-97,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 42824; Mature: 42693

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVITFNKDGNKRQVPLSGR
CCCCEEEEEECCCCCCCCEEEECCCCCCHHEEHHHHCCCCCEEEEECCCCCEEECCCCCC
NCHAGAVGMLLNELEKHGLHDRIKAVGHRIAHGGEKYSESVLIDQAVMDELNACIPLAPL
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEECCC
HNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTYAVPRELRKKYAFRRYGFHGT
CCCCCCCEEEEEHHHCCCCCCCCCCCCHHHHHCCHHHEEEHHHHHHHHHHHHHHCCCCCC
SMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKNGKSVDTSMGFTPIEGLVMGTRC
CEEEECCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCC
GDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGISELSNDCRTLEIAADEGHEGARLAL
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEHHHHCCCCEEEEEECCCCCCCHHHHH
EVMTYRLAKYIASMAVACGGVDALVFTGGIGENSRNIRAKTVSYLDFLGLHIDTKANMEK
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCEEECCCCCHHH
RYGNSGIISPTDSSPAVLVVPTNEELMIACDTAELVGIL
HCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHCCC
>Mature Secondary Structure 
SDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVITFNKDGNKRQVPLSGR
CCCEEEEEECCCCCCCCEEEECCCCCCHHEEHHHHCCCCCEEEEECCCCCEEECCCCCC
NCHAGAVGMLLNELEKHGLHDRIKAVGHRIAHGGEKYSESVLIDQAVMDELNACIPLAPL
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEECCC
HNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTYAVPRELRKKYAFRRYGFHGT
CCCCCCCEEEEEHHHCCCCCCCCCCCCHHHHHCCHHHEEEHHHHHHHHHHHHHHCCCCCC
SMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKNGKSVDTSMGFTPIEGLVMGTRC
CEEEECCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCC
GDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGISELSNDCRTLEIAADEGHEGARLAL
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEHHHHCCCCEEEEEECCCCCCCHHHHH
EVMTYRLAKYIASMAVACGGVDALVFTGGIGENSRNIRAKTVSYLDFLGLHIDTKANMEK
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCEEECCCCCHHH
RYGNSGIISPTDSSPAVLVVPTNEELMIACDTAELVGIL
HCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]