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The map label for this gene is pdxJ [H]

Identifier: 161870626

GI number: 161870626

Start: 1731692

End: 1732420

Strand: Reverse

Name: pdxJ [H]

Synonym: NMCC_1695

Alternate gene names: 161870626

Gene position: 1732420-1731692 (Counterclockwise)

Preceding gene: 161870627

Following gene: 161870625

Centisome position: 80.45

GC content: 56.65

Gene sequence:

>729_bases
ATGCTTTTAGGTGTCAACATCGACCACATCGCTACCGTCCGCAATGCGCGCGGCACGACTTATCCCAGCCCCGTGGAGGC
GGCACTGGTTGCCGAAACGCACGGTGCGGATTTGATTACCATGCACCTGCGCGAAGACCGCCGCCACATCAAAGACGCGG
ATGTGTTTGCCGTCAAAAACGCCATCCGCACGCGCCTGAACCTTGAAATGGCGTTGACGGAAGAAATGCTCGAAAACGCG
CTTAAAGTGATGCCGGAAGACGTGTGCATCGTGCCTGAAAAACGTCAGGAAATCACGACCGAAGGCGGCTTGGACGTATT
GGCGCAACAGGAAAAAATCGCCGGGTTCACCAAAATCCTGACCGACGCAGGCATCCGAGTCTCTCTCTTTATCGATGCCG
ACGACAGGCAAATCCAAGCCGCCTATGATGTCGGCGCGCCCGTTATCGAATTGCACACCGGCGCATATGCCGATGCACAC
AGCCACGCCGAGCAAATGAAACAATTCGAGCGCATTCAAAACGGTGCGCATTACGCCAGCGATTTGGGTTTGGTCGTTAA
CGCCGGACACGGCCTGACCATTCACAACGTTACGCCGATTGCCCAAATCCTCGCCATCCGCGAACTGAACATCGGGCATT
CGCTGATTGCCCAAGCCCTCTTCCTCGGACTGCCCGAAGCCGTGCGCCAAATGAAGGAGGCGATGTTCAGGGCAAGGCTG
CTGCCGTAA

Upstream 100 bases:

>100_bases
TACGGCAGTTTGACCGCAAAGAAACCGCCCGGGAAACCGTCCCGACTTCGGACGGCACGGCTTCAAATGCCGTCTGAAGG
CAGAAATAAAAGGAAAGATT

Downstream 100 bases:

>100_bases
GGGCAGGCAAACCCTTTCAGACAGCATTTCACGACAGGGATATGTTATAGTGGATTAAATTTAAATCAGGACAAGGCGGC
GAAGCCGCAGACAGTACAAA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLREDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENA
LKVMPEDVCIVPEKRQEITTEGGLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVIELHTGAYADAH
SHAEQMKQFERIQNGAHYASDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQALFLGLPEAVRQMKEAMFRARL
LP

Sequences:

>Translated_242_residues
MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLREDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENA
LKVMPEDVCIVPEKRQEITTEGGLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVIELHTGAYADAH
SHAEQMKQFERIQNGAHYASDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQALFLGLPEAVRQMKEAMFRARL
LP
>Mature_242_residues
MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLREDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENA
LKVMPEDVCIVPEKRQEITTEGGLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVIELHTGAYADAH
SHAEQMKQFERIQNGAHYASDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQALFLGLPEAVRQMKEAMFRARL
LP

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=239, Percent_Identity=61.0878661087866, Blast_Score=293, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 26604; Mature: 26604

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLREDRRHIKDADVFAVKN
CEEECCHHHHHHHHCCCCCCCCCCCHHEEEEECCCCCEEEEEEHHHHHHCCCHHHHHHHH
AIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEGGLDVLAQQEKIAGFTKIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
TDAGIRVSLFIDADDRQIQAAYDVGAPVIELHTGAYADAHSHAEQMKQFERIQNGAHYAS
HCCCCEEEEEEECCCCEEEEHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHH
DLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQALFLGLPEAVRQMKEAMFRARL
CCCEEEECCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHC
LP
CC
>Mature Secondary Structure
MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLREDRRHIKDADVFAVKN
CEEECCHHHHHHHHCCCCCCCCCCCHHEEEEECCCCCEEEEEEHHHHHHCCCHHHHHHHH
AIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEGGLDVLAQQEKIAGFTKIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
TDAGIRVSLFIDADDRQIQAAYDVGAPVIELHTGAYADAHSHAEQMKQFERIQNGAHYAS
HCCCCEEEEEEECCCCEEEEHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHH
DLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQALFLGLPEAVRQMKEAMFRARL
CCCEEEECCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHC
LP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA