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The map label for this gene is potD1 [H]

Identifier: 161870611

GI number: 161870611

Start: 1710985

End: 1712127

Strand: Direct

Name: potD1 [H]

Synonym: NMCC_1680

Alternate gene names: 161870611

Gene position: 1710985-1712127 (Clockwise)

Preceding gene: 161870603

Following gene: 161870614

Centisome position: 79.45

GC content: 53.28

Gene sequence:

>1143_bases
ATGAAAAAATCCGTATTAGCCGTTTTGGCCGCGCTGTCTCTGGCAGCCTGCGGCGGCAGCGAAAAAAACGCCGTGCAACC
TCAAGCAGACGCTGCGTCTGCTGCCAATGCCGAAGCCGCTGCCACCGATACTCTGAACATCTACAACTGGTCGAACTATG
TTGATGAGAGTACGGTCGAAGACTTTAAAAAAGCCAACAATCTGAAGCTCACTTACGACTTGTACGAAAACAATGAAACG
CTGGAAGCCAAAATGCTGACCGGCAAATCCGGTTACGATTTGGTTGTGCCGGGCATCGCCTTCCTACCGCGCCAAATCGA
GGCGGGCGCGTATCAAAAAGTCAACAAAGACCTGATTCCCAACTATAAAAACATCGATCCCGAACTCTTGAAAATGCTGG
AAACCGCCGACCCGGGCAACCAGTATGCCGTCCCCTATTTCTCCGGCGTGAACACGATTGCGATTACGGCGAAAGGCAAA
GAGCTTTTAGGCGGCAAGCTGCCCGAAAACGGCTGGGATTTGCTGTTCAAACCCGAATACACCCACAAGCTGAAATCCTG
CGGCATCGCCCTGTGGGACACCCCGAGCGAAATGTTCCCGATTTTGCTGAACTACTTGGGCAAAGACCCCAAAGGTTCGA
ATCCTGAAGATTTGAAGGCAGCGGCGGAAGTGTTGAAGTCTATCCGTCCGGATGTCAAACGTTTCAGCCCGTCCATCATC
GACGAGCTGGCGCGCGGCGACATCTGCCTGGCGGCAGGCAACGGCGGCGATTTGAACTTGGCGAAAGCACGTTCCGAAGA
AGTGAAAAACAACGTCGGCATCGAAGTGCTGAATCCGAAAGGCATGGGCTTCTGGATTGAGTCTTGGCTGATTCCCGCCG
ATGCGAAAAACGTCGCCAATGCCCACAAATACATCAACTACACGCTCGACCCCGAAATCGCGGCGAAAAACGGCATCGCC
GTAACCTTCGCCCCCGCCAGCAAACCGGCACGCGAAAAAATGCCTGCCGAACTGGTGAACACCCGTTCTATCTTCCCGAA
CGAGCAGGATATGAAAGACGGTTTCGTGATGCCGCAAATGAGTACGGATGCGAAAAAACTGTCGGTCAGCCTGTGGCAGA
AAATCAAAGTCGGCACCAACTGA

Upstream 100 bases:

>100_bases
TGCACAAATTTACTTTACAATGCCCAAACCTTGCGCCGGGGTGTTTATGGGGCGCGGGGTTTTCTTTTTTTCCAACCTCT
TTCATCAGGAATCTGCCCGT

Downstream 100 bases:

>100_bases
TTTGAAGCATTAAAAATGCCGTCCGAACGATGTTCGGACGGCATTTTATATTGGATTCAAATAGAAAATATTTATACCGT
TATGCCGGCGGCCGGAATTT

Product: spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDTLNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNET
LEAKMLTGKSGYDLVVPGIAFLPRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDLKAAAEVLKSIRPDVKRFSPSII
DELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEVLNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIA
VTFAPASKPAREKMPAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN

Sequences:

>Translated_380_residues
MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDTLNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNET
LEAKMLTGKSGYDLVVPGIAFLPRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDLKAAAEVLKSIRPDVKRFSPSII
DELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEVLNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIA
VTFAPASKPAREKMPAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN
>Mature_380_residues
MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDTLNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNET
LEAKMLTGKSGYDLVVPGIAFLPRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDLKAAAEVLKSIRPDVKRFSPSII
DELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEVLNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIA
VTFAPASKPAREKMPAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=344, Percent_Identity=39.2441860465116, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1787367, Length=347, Percent_Identity=31.1239193083574, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41358; Mature: 41358

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDTLNIYNWSNYVDESTVE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCHHHHH
DFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFLPRQIEAGAYQKVNKDLIP
HHHHCCCCEEEEEEECCCCEEEEEEEECCCCCEEEECCHHHHHHHHCCCHHHHHCHHHCC
NYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGKELLGGKLPENGWDLLFKPEY
CCCCCCHHHHHHHHCCCCCCCEECEEECCCCEEEEEECCHHHHCCCCCCCCCCEEECCHH
THKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDLKAAAEVLKSIRPDVKRFSPSII
HHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCHHHH
DELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEVLNPKGMGFWIESWLIPADAKNVAN
HHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEHEECCCCHHHHHH
AHKYINYTLDPEIAAKNGIAVTFAPASKPAREKMPAELVNTRSIFPNEQDMKDGFVMPQM
HHHEEEEEECCCHHCCCCEEEEECCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCEECCCC
STDAKKLSVSLWQKIKVGTN
CCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDTLNIYNWSNYVDESTVE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCHHHHH
DFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFLPRQIEAGAYQKVNKDLIP
HHHHCCCCEEEEEEECCCCEEEEEEEECCCCCEEEECCHHHHHHHHCCCHHHHHCHHHCC
NYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGKELLGGKLPENGWDLLFKPEY
CCCCCCHHHHHHHHCCCCCCCEECEEECCCCEEEEEECCHHHHCCCCCCCCCCEEECCHH
THKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDLKAAAEVLKSIRPDVKRFSPSII
HHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCHHHH
DELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEVLNPKGMGFWIESWLIPADAKNVAN
HHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEHEECCCCHHHHHH
AHKYINYTLDPEIAAKNGIAVTFAPASKPAREKMPAELVNTRSIFPNEQDMKDGFVMPQM
HHHEEEEEECCCHHCCCCEEEEECCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCEECCCC
STDAKKLSVSLWQKIKVGTN
CCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]