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The map label for this gene is gdhA [H]

Identifier: 161870555

GI number: 161870555

Start: 1649783

End: 1651117

Strand: Direct

Name: gdhA [H]

Synonym: NMCC_1621

Alternate gene names: 161870555

Gene position: 1649783-1651117 (Clockwise)

Preceding gene: 161870551

Following gene: 161870556

Centisome position: 76.61

GC content: 55.73

Gene sequence:

>1335_bases
ATGACTGACCTGAACACCCTGTTTGCCAACCTCAAACAACGCAATCCCAATCAGGAGCCGTTCCATCAGGCGGTTGAAGA
AGTCTTCATGAGTCTCGATCCGTTTTTGGCAAAAAATCCGAAATACACCCAGCAAAGCCTGCTGGAACGCATCGTCGAAC
CCGAACGCGTCGTCATGTTCCGCGTAACCTGGCAGGACGATAAAGGGCAAGTCCAAGTCAACCGGGGCTACCGCGTGCAA
ATGAGTTCCGCCATCGGTCCTTACAAAGGCGGCCTGCGCTTCCATCCGACCGTCGATTTGGGCGTATTGAAATTCCTCGC
TTTTGAACAAGTGTTCAAAAACGCCTTGACCACCCTGCCTATGGGCGGCGGCAAAGGCGGTTCCGACTTCGACCCCAAAG
GCAAATCCGATGCCGAAGTAATGCGCTTCTGCCAAGCCTTTATGACCGAACTCTACCGCCACATCGGCGCGGACACCGAT
GTTCCGGCCGGCGACATCGGCGTAGGCGGGCGCGAAATCGGCTACCTGTTCGGACAATACAAAAAAATCCGCAACGAGTT
TTCTTCCGTCCTAACCGGCAAAGGTTTGGAATGGGGCGGCAGCCTCATCCGTCCCGAAGCGACCGGCTACGGCTGCGTCT
ATTTCGCCCAAGCGATGCTGCAAACCCGCAACGATAGTTTTGAAGGCAAACGCGTCCTGATTTCCGGTTCCGGCAACGTG
GCGCAATACGCCGCCGAAAAAGCCATCCAACTGGGTGCGAAAGTACTGACCGTTTCCGACTCCAACGGCTTCGTCCTCTT
CCCCGACAGCGGTATGACCGAAGCGCAACTCGCCGCCTTGATCGAATTGAAAGAAGTCCGCCGCGAACGCGTTGCCACCT
ACGCCAAAGAGCAAGGTCTGCAATACTTTGAAAAACAAAAACCGTGGGGCGTCGCCGCCGAAATCGCCCTGCCCTGCGCG
ACCCAGAACGAATTGGACGAAGAAGCCGCCAAAACCCTGTTGGCAAACGGCTGCTACGTCGTTGCCGAAGGTGCGAATAT
GCCGTCGACTTTGGGCGCGGTCGAGCAATTTATCAAAGCCGGCATCCTCTACGCCCCGGGAAAAGCCTCCAATGCCGGCG
GCGTAGCAACTTCAGGTTTGGAAATGAGCCAAAACGCCATCCGCCTGTCTTGGACTCGTGAAGAAGTCGACCAACGCCTG
TTCGGCATCATGCAAAGCATCCACGAATCCTGTCTGAAATACGGCAAAGTCGGCGACACAGTAAACTACGTCAATGGTGC
GAACATTGCCGGTTTCGTCAAAGTTGCCGATGCGATGCTGGCGCAAGGCTTCTAA

Upstream 100 bases:

>100_bases
TTTGCCAAATCGGGCTTGGCATCAGAAAAAAATAGGTTTATATTCCCACCTACAAATTTGTTTTCCCATTAGTACACTAT
CAACCAAAAGGAGTATCCGA

Downstream 100 bases:

>100_bases
GCAAACGCCGCCGCTCCGCAAACAAAATGCCGTCCGAACCGCAAATGCTGTTCAGACGGCATTTCATTATCCGCCCGTTC
GAATCGGGTGATACTATTTG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQQSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQ
MSSAIGPYKGGLRFHPTVDLGVLKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQAMLQTRNDSFEGKRVLISGSGNV
AQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQLAALIELKEVRRERVATYAKEQGLQYFEKQKPWGVAAEIALPCA
TQNELDEEAAKTLLANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWTREEVDQRL
FGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF

Sequences:

>Translated_444_residues
MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQQSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQ
MSSAIGPYKGGLRFHPTVDLGVLKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQAMLQTRNDSFEGKRVLISGSGNV
AQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQLAALIELKEVRRERVATYAKEQGLQYFEKQKPWGVAAEIALPCA
TQNELDEEAAKTLLANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWTREEVDQRL
FGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF
>Mature_443_residues
TDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQQSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQM
SSAIGPYKGGLRFHPTVDLGVLKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTDV
PAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQAMLQTRNDSFEGKRVLISGSGNVA
QYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQLAALIELKEVRRERVATYAKEQGLQYFEKQKPWGVAAEIALPCAT
QNELDEEAAKTLLANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWTREEVDQRLF
GIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=403, Percent_Identity=29.2803970223325, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI4885281, Length=403, Percent_Identity=28.7841191066998, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1788059, Length=440, Percent_Identity=67.0454545454545, Blast_Score=613, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI17544676, Length=393, Percent_Identity=27.2264631043257, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324951, Length=453, Percent_Identity=53.2008830022075, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319256, Length=455, Percent_Identity=52.5274725274725, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24649283, Length=360, Percent_Identity=29.7222222222222, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45553475, Length=402, Percent_Identity=26.865671641791, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45549226, Length=373, Percent_Identity=27.3458445040214, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24649460, Length=297, Percent_Identity=27.9461279461279, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48491; Mature: 48360

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQQSLLERIVEPERVVMF
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCCCCEEEEE
RVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGVLKFLAFEQVFKNALTTLP
EEEEECCCCCEEECCCEEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCC
MGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTDVPAGDIGVGGREIGYLFGQY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
KKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQAMLQTRNDSFEGKRVLISGSGNV
HHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCH
AQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQLAALIELKEVRRERVATYAKEQGL
HHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYFEKQKPWGVAAEIALPCATQNELDEEAAKTLLANGCYVVAEGANMPSTLGAVEQFIKA
HHHHHCCCCCCCHHEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHC
GILYAPGKASNAGGVATSGLEMSQNAIRLSWTREEVDQRLFGIMQSIHESCLKYGKVGDT
CEEECCCCCCCCCCCEECCCHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VNYVNGANIAGFVKVADAMLAQGF
CCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQQSLLERIVEPERVVMF
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCCCCEEEEE
RVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGVLKFLAFEQVFKNALTTLP
EEEEECCCCCEEECCCEEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCC
MGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTDVPAGDIGVGGREIGYLFGQY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
KKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQAMLQTRNDSFEGKRVLISGSGNV
HHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCH
AQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQLAALIELKEVRRERVATYAKEQGL
HHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYFEKQKPWGVAAEIALPCATQNELDEEAAKTLLANGCYVVAEGANMPSTLGAVEQFIKA
HHHHHCCCCCCCHHEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHC
GILYAPGKASNAGGVATSGLEMSQNAIRLSWTREEVDQRLFGIMQSIHESCLKYGKVGDT
CEEECCCCCCCCCCCEECCCHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VNYVNGANIAGFVKVADAMLAQGF
CCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]