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The map label for this gene is rep [H]

Identifier: 161870316

GI number: 161870316

Start: 1373202

End: 1375274

Strand: Direct

Name: rep [H]

Synonym: NMCC_1358

Alternate gene names: 161870316

Gene position: 1373202-1375274 (Clockwise)

Preceding gene: 161870315

Following gene: 161870320

Centisome position: 63.77

GC content: 53.3

Gene sequence:

>2073_bases
GTGCGACACTCGACTGCCTCGAAAACGGCGCAGACTGATTTCAGACAGCATCAAAACATGATGAAACTCAATCCCCAACA
GCTCGAAGCCGTCCGCTACCTCGGCGGCCCACTGCTCGTCCTTGCCGGTGCAGGCAGCGGCAAAACCGGCGTGATTACTC
AAAAAATCAAGTATTTGATTGTCAATGTCGGCTATCTGCCGCATACCGTTGCCGCGATTACCTTTACCAATAAAGCCGCC
GCGGAAATGCAGGAGCGCGTCGCCAAAATGCTGCACAAATCGCAAACGCGCGGGCTGACGATTTGCACGTTCCACTCTTT
GGGCATGAAGATTCTGCGCGAAGAGGCGAACCATATCGGTTACAAAAAAAACTTCTCCATTCTCGATTCTACCGACAGCG
CGAAAATCATCGGCGAACTCTTAGGCGGTACGGGCAAAGAAGCCGTATTCAAGGCGCAGCACCAGATTTCCTTGTGGAAA
AACGATTTAAAAACGCCTGAAGATGTCGTTCAGACGGCCTCTAATGCGTGGGAACAACAAACCGCACGCGTGTATGCGAG
CTATCAGGAAACCTTACAAAGCTATCAGGCAGTGGACTTCGACGACTTGATCCGCCTGCCTGCCGTGCTGTTGCAGCAAA
ACAGCGAAGTGCGCAACAAATGGCAGCGGCGGCTGCGTTATCTGCTGGTTGACGAATGCCAAGATACGAATACCTGCCAA
TTTACGTTGATGAAACTTCTGACCGGCGCGGAAGGTATGTTTACCGCCGTCGGCGACGACGACCAGTCCATCTACGCATG
GCGCGGCGCGAACATGGAAAACCTGCGCAAAATGCAGGAAAACTATCCGCAGATGAAGGTCATCAAACTGGAGCAAAACT
ACCGCTCCACCGCGCGGATTCTCAAAATCGCCAACAAAGTCATCGAGAACAACCCCAAGCTGTTCACCAAAAAACTTTGG
TCGAAATTGGGCGAAGGCGAGCCCGTCAAAGTCGTTGCCTGCCAAAACGAGCAGCACGAAGCCGACTGGGTCGTCAGCCA
AATCGTCAAACAAAAACTCATCGGCGGCGACAAAACCCAATATGCCGATTTCGCCGTGTTGTACCGCGGAAAGCATCAGG
CGAGGATTTTCGAGGAAGCATTGCGCGGCGCGCGCATCCCCTACCGTCTCTCCGGCGGACAAAGCTTTTTCGACAAAGCC
GAAATCAAAGACGTGTTGTCTTATGTGCGGCTGCTTGCCAACCCCAATGACGATCCCGCCTTTCTGCGTGCCGTTACCAC
GCCCAAACGCGGCATCGGCGATGTCACGCTGGGCAAGCTCAACACTTACGCGCACGAACACGAATGCAGCCTGTATGAAG
CCGCACAAAACGAAGAAGCCCTTGCCACGCTGAACAATACCAACCGCCAACACCTGCAAGCCTTTATGGATATGTTCGGC
AGCTACCGCGCCAAAGCGGAAACCGGCGAAGCAGGCGAGTTCATCAACAGCCTGCTCGAAGAAATCGACTATGAAAACCA
TTTGATGCAAAACGAAGAAGGCAAGGCCGGCGAAATCAAATGGCGCAATGTCAGCGATTTGGTATCATGGTTTGCGCGAA
AAGGCGGGGAAGACGGCAAAAACATCATCGAACTCGCCCAAACCGTCGCCTTGATGACGCTTTTGGAAGGAAAAGACGAA
GAAGAAACCGATGCCGTCTCGCTATCCACGCTACACGCCGCCAAAGGTTTGGAGTATCCGTATGTTTTCCTTGTCGGTTG
CGAAGAAGGCGTTTTGCCGCACAACGACAGTATCGAAGAGGGCAACGTCGAAGAAGAACGCCGCCTGATGTACGTCGGCA
TCACCCGCGCCAAACGCCAACTCACGCTGACCCACTGCGTCAAACGCAAAAAACAAGGCACATGGCAGTTCCCCGAACCC
AGCCGATTCATAGACGAAATGCCGCAGGAAGATTTGAAAATCCTGGGGCGCAAAGGCGGCGAGCCGATTGTCAGCAAGGA
AGAAGGCAGACGCAACCTTGCCGATATAATCGGAAGGCTCGACAACCTAAAAAAAAGCGGCGCGGCGGATTAA

Upstream 100 bases:

>100_bases
GCGGTCAGCCGGCTTGCCGGACAGGTGTTCGGCTCAAGCATCACGTTCGGTTCGGGAACGCAAAACTCCGCGCCGGGGCA
AATCGGCGTATCCGCCCTCC

Downstream 100 bases:

>100_bases
ACCGGAGCCGCAATGCCGTCTGAAGGCTTCAGACGGCATATTTTTTGGACGGCGCGCGTAAAGCGGTTTACGCCCACAAA
TCCTGCTGCTGGTTTTTCGG

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MRHSTASKTAQTDFRQHQNMMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNVGYLPHTVAAITFTNKAA
AEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIGYKKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWK
NDLKTPEDVVQTASNAWEQQTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTNTCQ
FTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYRSTARILKIANKVIENNPKLFTKKLW
SKLGEGEPVKVVACQNEQHEADWVVSQIVKQKLIGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKA
EIKDVLSYVRLLANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNRQHLQAFMDMFG
SYRAKAETGEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVSDLVSWFARKGGEDGKNIIELAQTVALMTLLEGKDE
EETDAVSLSTLHAAKGLEYPYVFLVGCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEP
SRFIDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD

Sequences:

>Translated_690_residues
MRHSTASKTAQTDFRQHQNMMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNVGYLPHTVAAITFTNKAA
AEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIGYKKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWK
NDLKTPEDVVQTASNAWEQQTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTNTCQ
FTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYRSTARILKIANKVIENNPKLFTKKLW
SKLGEGEPVKVVACQNEQHEADWVVSQIVKQKLIGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKA
EIKDVLSYVRLLANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNRQHLQAFMDMFG
SYRAKAETGEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVSDLVSWFARKGGEDGKNIIELAQTVALMTLLEGKDE
EETDAVSLSTLHAAKGLEYPYVFLVGCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEP
SRFIDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD
>Mature_690_residues
MRHSTASKTAQTDFRQHQNMMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNVGYLPHTVAAITFTNKAA
AEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIGYKKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWK
NDLKTPEDVVQTASNAWEQQTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTNTCQ
FTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYRSTARILKIANKVIENNPKLFTKKLW
SKLGEGEPVKVVACQNEQHEADWVVSQIVKQKLIGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKA
EIKDVLSYVRLLANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNRQHLQAFMDMFG
SYRAKAETGEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVSDLVSWFARKGGEDGKNIIELAQTVALMTLLEGKDE
EETDAVSLSTLHAAKGLEYPYVFLVGCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEP
SRFIDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=641, Percent_Identity=48.6739469578783, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI2367296, Length=666, Percent_Identity=37.2372372372372, Blast_Score=414, Evalue=1e-116,
Organism=Escherichia coli, GI1787196, Length=386, Percent_Identity=26.1658031088083, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6322369, Length=470, Percent_Identity=31.4893617021277, Blast_Score=179, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6324477, Length=691, Percent_Identity=21.8523878437048, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77857; Mature: 77857

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHSTASKTAQTDFRQHQNMMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLI
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHH
VNVGYLPHTVAAITFTNKAAAEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIG
EECCCCCHHEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCC
YKKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQQ
CCCCCCEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTNTCQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHH
FTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYRSTARI
HHHHHHHHCCCCEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
LKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKLIGGDKTQ
HHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCH
YADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRLLANPNDDPA
HHHHHHEECCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
FLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNRQHLQAFMDMFG
HEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHH
SYRAKAETGEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVSDLVSWFARKGGEDGK
HHCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
NIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLVGCEEGVLPHNDSIEE
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
GNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRFIDEMPQEDLKILGRKGG
CCCHHHHEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHCCCC
EPIVSKEEGRRNLADIIGRLDNLKKSGAAD
CCCCCCHHCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRHSTASKTAQTDFRQHQNMMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLI
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHH
VNVGYLPHTVAAITFTNKAAAEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIG
EECCCCCHHEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCC
YKKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQQ
CCCCCCEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTNTCQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHH
FTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYRSTARI
HHHHHHHHCCCCEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
LKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKLIGGDKTQ
HHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCH
YADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRLLANPNDDPA
HHHHHHEECCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
FLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNRQHLQAFMDMFG
HEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHH
SYRAKAETGEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVSDLVSWFARKGGEDGK
HHCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
NIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLVGCEEGVLPHNDSIEE
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
GNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRFIDEMPQEDLKILGRKGG
CCCHHHHEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHCCCC
EPIVSKEEGRRNLADIIGRLDNLKKSGAAD
CCCCCCHHCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]