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The map label for this gene is iscS [H]

Identifier: 161870252

GI number: 161870252

Start: 1295090

End: 1296304

Strand: Direct

Name: iscS [H]

Synonym: NMCC_1293

Alternate gene names: 161870252

Gene position: 1295090-1296304 (Clockwise)

Preceding gene: 161870250

Following gene: 161870253

Centisome position: 60.14

GC content: 55.88

Gene sequence:

>1215_bases
ATGACCGTCAAAACCCCCGTTTACCTCGACTACGCCGCCACTACCCCCGTTGACAAACGCGTTGCCGAAAAAATGATTCC
CTATCTGACCGAAACCTTCGGCAACCCCGCCTCCAACAGCCACGCATTCGGCTGGGAAGCAGAAGAGGCCGTCGAAAAAG
CCCGCGCCGACATCGCTGCCCTGATTAATGCCGACCCCAAAGAAATCGTCTTCACCAGCGGCGCGACCGAGTCCGACAAC
CTCGCCATCAAAGGCGCGGCAAACTTCTACAAAACCAAAGGCAAACACCTCATTACCGTCAAAACCGAACATAAAGCCGT
TTTAGATACAATGCGCGAACTCGAACGCCAAGGTTTTGAAGTAACCTACCTCGGCGTTCAAGAAAACGGTTTGATTGATT
TGGAAGAACTCAAAGCCGCCATCCGCGACGACACCATCCTGATTTCCGTAATGTGGGTAAACAACGAAATCGGCGTGGTG
CAAGACATTCCCGCCATCGGCGAAATCTGCCGCGAACGTAAAATCGTCTTCCACGTCGATGCCGCCCAAGCCTGCGGCAA
AGTGCCTGTCGATGTCGAAGCCGCCAAAATCGACCTGCTCTCGATGTCCGCGCACAAAGTGTACGGCCCCAAAGGCATCG
GCGCGCTGTACGTCCGCCGCAAACCCCGCGTCCGCCTCGAAGCCCAAATGCACGGCGGCGGCCACGAACGCGGCTTCCGC
AGCGGCACATTGCCGACCCACCAAATCGTCGGTATGGGCGAAGCCTTCCGCATCGCCAAAGAAGAACTCGAACAAGATAT
GGCGCACTACCGCAAACTGCGCGACATCTTCCTCAAAGGCATCGAAGGCATCGAAGAAGTCTATGTCAACGGCGACCTCG
AACACCGCGCCCCGAACAACCTGAACGTCAGCTTCAACTTCGTCGAAGGCGAAAGCCTGATTATGGCGGTGAAAGAACTC
GCCGTATCCAGCGGCTCCGCCTGTACTTCTGCCAGTCTCGAACCAAGCTACGTCCTACGCGCGCTCGGTCGCAACGACGA
ACTGGCGCACTCATCCCTGCGCATCACTTTCGGCCGCATGACCACCGAAGAAGAAGTGCAATTCGCCGCCGAACTGATTA
AATCCAAAATCGGCAAACTGCGCGAACTGTCGCCGCTGTGGGAAATGTTCAAAGACGGGATTGATTTAAACTCAATTGAA
TGGGCGGCGCATTGA

Upstream 100 bases:

>100_bases
CTGCAAAGCATCATCGAACAGAAAAACAACGGCGACGGCAGCCGCGTCGTCCAATTTACACACATCCATTAAATAACACC
CGAAAAAGAAAGAGCAAACC

Downstream 100 bases:

>100_bases
TTAATGTACTTTATTGTGTAGGTTGAGTGCTTAAATCAAATTTTAGTGGAAATATTATTTATTACTGGTAAATAAAAAGG
GGAGTTGAATTATGTCCAAT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFGWEAEEAVEKARADIAALINADPKEIVFTSGATESDN
LAIKGAANFYKTKGKHLITVKTEHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWVNNEIGVV
QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYVRRKPRVRLEAQMHGGGHERGFR
SGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDIFLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKEL
AVSSGSACTSASLEPSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKDGIDLNSIE
WAAH

Sequences:

>Translated_404_residues
MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFGWEAEEAVEKARADIAALINADPKEIVFTSGATESDN
LAIKGAANFYKTKGKHLITVKTEHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWVNNEIGVV
QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYVRRKPRVRLEAQMHGGGHERGFR
SGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDIFLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKEL
AVSSGSACTSASLEPSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKDGIDLNSIE
WAAH
>Mature_403_residues
TVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFGWEAEEAVEKARADIAALINADPKEIVFTSGATESDNL
AIKGAANFYKTKGKHLITVKTEHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWVNNEIGVVQ
DIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYVRRKPRVRLEAQMHGGGHERGFRS
GTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDIFLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKELA
VSSGSACTSASLEPSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKDGIDLNSIEW
AAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=403, Percent_Identity=58.8089330024814, Blast_Score=514, Evalue=1e-146,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=33.8164251207729, Blast_Score=208, Evalue=6e-54,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=73.7623762376238, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=287, Percent_Identity=27.5261324041812, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789175, Length=237, Percent_Identity=30.8016877637131, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=57.6059850374065, Blast_Score=498, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17533177, Length=321, Percent_Identity=33.6448598130841, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=58.8972431077694, Blast_Score=508, Evalue=1e-145,
Organism=Drosophila melanogaster, GI20129463, Length=400, Percent_Identity=57.5, Blast_Score=483, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44739; Mature: 44607

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFGWEAEEAVEKARADIAA
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKTEHKAVLDTMRELERQGFE
HHCCCCHHEEEECCCCCCCCEEEEECCHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCE
VTYLGVQENGLIDLEELKAAIRDDTILISVMWVNNEIGVVQDIPAIGEICRERKIVFHVD
EEEEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHCCHHHHHHHHCCEEEEEEE
AAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYVRRKPRVRLEAQMHGGGHERGFR
HHHHCCCCCCCCCHHEEEEEECCHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
SGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDIFLKGIEGIEEVYVNGDLEHRAPNN
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCC
LNVSFNFVEGESLIMAVKELAVSSGSACTSASLEPSYVLRALGRNDELAHSSLRITFGRM
CEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCC
TTEEEVQFAAELIKSKIGKLRELSPLWEMFKDGIDLNSIEWAAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFGWEAEEAVEKARADIAA
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKTEHKAVLDTMRELERQGFE
HHCCCCHHEEEECCCCCCCCEEEEECCHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCE
VTYLGVQENGLIDLEELKAAIRDDTILISVMWVNNEIGVVQDIPAIGEICRERKIVFHVD
EEEEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHCCHHHHHHHHCCEEEEEEE
AAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYVRRKPRVRLEAQMHGGGHERGFR
HHHHCCCCCCCCCHHEEEEEECCHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
SGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDIFLKGIEGIEEVYVNGDLEHRAPNN
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCC
LNVSFNFVEGESLIMAVKELAVSSGSACTSASLEPSYVLRALGRNDELAHSSLRITFGRM
CEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCC
TTEEEVQFAAELIKSKIGKLRELSPLWEMFKDGIDLNSIEWAAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA