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The map label for this gene is gatB [H]

Identifier: 161870228

GI number: 161870228

Start: 1268200

End: 1269630

Strand: Direct

Name: gatB [H]

Synonym: NMCC_1269

Alternate gene names: 161870228

Gene position: 1268200-1269630 (Clockwise)

Preceding gene: 161870227

Following gene: 161870229

Centisome position: 58.89

GC content: 54.37

Gene sequence:

>1431_bases
ATGACCTGGGAAACCGTAATCGGCTTGGAAATCCACGTCCAATTGAACACCAAATCCAAAATCTTCAGCGGCGCATCGAC
CGCATTCGGCGCAGAACCCAACGCGCACGCCAGCGTAGTGGAGTGCGCGCTGCCGGGCGTACTGCCGGTAATGAACCGAG
AAGTCGTTGAAAAAGCCATCAAATTGGGTTTGGCTTTAGATGCGAAAATCAATCAGAAAAACGTGTTCGACCGTAAAAAC
TACTTCTATCCCGACTTACCAAAAGGCTATCAAATCAGCCAGTTGGACTTACCGATTGTCGAACACGGCAAATTGGAAAT
CGTAGTCGGCGACGATGTGAAAACCATCAACGTAACCCGTGCGCACATGGAAGAAGACGCAGGCAAGTCCGTGCATGAAG
GCTTGAACAGCGCAACCGGTATCGACCTGAACCGCGCCGGCACGCCGCTGTTGGAAGTCGTATCCGAACCCGAAATGCAC
TCCGCCGCCGAAGCCGTTGCCTACGCCAAAGCCTTACACAGCTTGGTAACCTGGCTGGACATTTGCGACGGCAATATGGC
GGAAGGTTCGTTCCGCGTCGATGCCAACGTATCCGTTCGCCCGAAAGGCCAAGCAGAGTTCGGCACGCGCCGCGAGATTA
AAAACCTCAATTCCTTCCGCTTCTTGGAGCAGGCAATTAATTACGAAGTGGAAGCGCAAATCGAGATTTTGGAAGACGGC
GGCAAAGTACAGCAGGCAACCATGCTGTTTGATCCCGAAAAAGGCGAAACCCGCGTGATGCGCCTGAAAGAAGACGCGCA
AGACTACCGCTACTTCCCCGACCCCGATTTGCTGCCCGTCATCATTTCAGACGCTCAAATGCAAAAAGCCAAAGCAGAAA
TGCCCGAGCTGCCGAAAGAAATGGCGGCACGTTTCGTGGCGGATTACGGTGTGAGCGACTACGACGCACGCCTGCTCACC
GCCAGCCGCGCACAGGCCGCCTATTTTGAAGAAGCCGCCAAAGAGAGCGGACAAGGCAAGCCGACTGCCAACTGGATGAA
CGGCGAACTCGCCGCCGCACTGAACAAAGCCGGTCTGGAACTTGCCGACAGCCCGATTACCGCCCCGCGCCTCGCCGCGC
TGGTAGGCAAAATCGCCGACGGCACATTAAGCAGCAAGTTAGCGAAAAAAGCCTTTGAAGCCATGTGGGCAGAACCCGAA
GCCACCATTGCCGAAATCATTGAAAAACACGGCTTGCAGCAAATGACCGATACCGGCGCAGTTGAAGCCATTGTGGACGA
AGTGCTGGCAAACAACGCCAAAGCCGTGGAACAATTCAAATCCGGCAACGAAAAAGCCCTGAATGCGATTGTGGGGCAAG
TGATGAAGGCCAGCAAAGGCAAAGCGAATCCCGCGCAGGTTCAAGAGCTGATTAAAGCCAAACTGGCTTAA

Upstream 100 bases:

>100_bases
AGCACGCGGCATTTCAGACGGCATCCAAACGGAAAAACCGCTCCGCCGGACAGCCTGACGCGAAGACTATCGAATTGATA
TTTTAGAGAAAGAAGCTCTT

Downstream 100 bases:

>100_bases
TCCGTTATCACACAGGTCGTCTGAAAGCAAAGTTCCAACGAAGGTAAAACAGGAAATAAGCTTTCAGACGGCCTTTTATA
GTGGATTAAATTTAAACCAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECALPGVLPVMNREVVEKAIKLGLALDAKINQKNVFDRKN
YFYPDLPKGYQISQLDLPIVEHGKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNSATGIDLNRAGTPLLEVVSEPEMH
SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNSFRFLEQAINYEVEAQIEILEDG
GKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPDLLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSDYDARLLT
ASRAQAAYFEEAAKESGQGKPTANWMNGELAAALNKAGLELADSPITAPRLAALVGKIADGTLSSKLAKKAFEAMWAEPE
ATIAEIIEKHGLQQMTDTGAVEAIVDEVLANNAKAVEQFKSGNEKALNAIVGQVMKASKGKANPAQVQELIKAKLA

Sequences:

>Translated_476_residues
MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECALPGVLPVMNREVVEKAIKLGLALDAKINQKNVFDRKN
YFYPDLPKGYQISQLDLPIVEHGKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNSATGIDLNRAGTPLLEVVSEPEMH
SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNSFRFLEQAINYEVEAQIEILEDG
GKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPDLLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSDYDARLLT
ASRAQAAYFEEAAKESGQGKPTANWMNGELAAALNKAGLELADSPITAPRLAALVGKIADGTLSSKLAKKAFEAMWAEPE
ATIAEIIEKHGLQQMTDTGAVEAIVDEVLANNAKAVEQFKSGNEKALNAIVGQVMKASKGKANPAQVQELIKAKLA
>Mature_475_residues
TWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECALPGVLPVMNREVVEKAIKLGLALDAKINQKNVFDRKNY
FYPDLPKGYQISQLDLPIVEHGKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNSATGIDLNRAGTPLLEVVSEPEMHS
AAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNSFRFLEQAINYEVEAQIEILEDGG
KVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPDLLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSDYDARLLTA
SRAQAAYFEEAAKESGQGKPTANWMNGELAAALNKAGLELADSPITAPRLAALVGKIADGTLSSKLAKKAFEAMWAEPEA
TIAEIIEKHGLQQMTDTGAVEAIVDEVLANNAKAVEQFKSGNEKALNAIVGQVMKASKGKANPAQVQELIKAKLA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=38.989898989899, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI115534094, Length=477, Percent_Identity=31.8658280922432, Blast_Score=221, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=503, Percent_Identity=31.013916500994, Blast_Score=212, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45550808, Length=481, Percent_Identity=35.966735966736, Blast_Score=312, Evalue=4e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51687; Mature: 51556

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECALPGVLPVMNREVVEKAI
CCCCEEEEEEEEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
KLGLALDAKINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEHGKLEIVVGDDVKTINVTR
HHCCCEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEECCCEEEEEECCCCEEEEHHH
AHMEEDAGKSVHEGLNSATGIDLNRAGTPLLEVVSEPEMHSAAEAVAYAKALHSLVTWLD
HHHHHHCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNSFRFLEQAINYEVEAQIEILEDG
HCCCCCCCCEEEEECCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHCCCEEEEEEEECCC
GKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPDLLPVIISDAQMQKAKAEMPELPKE
CCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHCCCCHHH
MAARFVADYGVSDYDARLLTASRAQAAYFEEAAKESGQGKPTANWMNGELAAALNKAGLE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCE
LADSPITAPRLAALVGKIADGTLSSKLAKKAFEAMWAEPEATIAEIIEKHGLQQMTDTGA
ECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCHH
VEAIVDEVLANNAKAVEQFKSGNEKALNAIVGQVMKASKGKANPAQVQELIKAKLA
HHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECALPGVLPVMNREVVEKAI
CCCEEEEEEEEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
KLGLALDAKINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEHGKLEIVVGDDVKTINVTR
HHCCCEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEECCCEEEEEECCCCEEEEHHH
AHMEEDAGKSVHEGLNSATGIDLNRAGTPLLEVVSEPEMHSAAEAVAYAKALHSLVTWLD
HHHHHHCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNSFRFLEQAINYEVEAQIEILEDG
HCCCCCCCCEEEEECCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHCCCEEEEEEEECCC
GKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPDLLPVIISDAQMQKAKAEMPELPKE
CCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHCCCCHHH
MAARFVADYGVSDYDARLLTASRAQAAYFEEAAKESGQGKPTANWMNGELAAALNKAGLE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCE
LADSPITAPRLAALVGKIADGTLSSKLAKKAFEAMWAEPEATIAEIIEKHGLQQMTDTGA
ECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCHH
VEAIVDEVLANNAKAVEQFKSGNEKALNAIVGQVMKASKGKANPAQVQELIKAKLA
HHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA