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The map label for this gene is aceF [H]

Identifier: 161870215

GI number: 161870215

Start: 1250518

End: 1252110

Strand: Direct

Name: aceF [H]

Synonym: NMCC_1255

Alternate gene names: 161870215

Gene position: 1250518-1252110 (Clockwise)

Preceding gene: 161870214

Following gene: 161870216

Centisome position: 58.07

GC content: 56.06

Gene sequence:

>1593_bases
ATGAGTATCGTAGAAATCAAAGTCCCCGATATCGGCGGTCACGAAAACGTCGACATCATCGCCGTAGAAGTTAAAGCGGG
CGACACCATCGCCGTTGACGACACCCTGATTACACTGGAAACCGACAAAGCCACGATGGATGTGCCTGCCGATGCGGCCG
GTGTCGTGAAAGAAGTAAAAGTCAAAGTCGGCGACAAAATCTCCGAAGGCGGCGTAATTTTGACCGTTGAAACCGGTGCC
GCCGCTGCCGAAGCCGCTCCTGCTGCTGCCGAAGCACAACCTGCACCTGCTGCCGCACCCGTTGCCGCAGGCGGTGCAAC
CGTTCAAGTAGCCGTTCCCGATATCGGCGGCCATACCGATGTGGATGTAATCGCCGTTGAAATCAAAGTGGGCGACACCG
TTGCCGAAGACGACACGCTGATTACTTTGGAAACCGATAAAGCGACAATGGACGTACCTTGTACCGCTGCCGGTGTCGTT
AAAGCCGTATTCTTAAAAGTCGGCGACAAAGTATCCGAAGGCTCTGCCATTATCGAAGTAGAAACCGTCGGCTCTGCCGC
AGCAGCCCCTGCTCAAGCCGCTCAAGCTGCCGCACCGGCTGCCGCTCCGCCTCCGACTGCTGCCGCCGCACCCGCCGCCG
CGCCTGCCGCTGCCAAAATCGACGAGGCCGCTTTCGCCAAAGCACACGCCGGCCCTTCCGCACGCAAACTGGCGCGCGAA
TTGGGCGTGGATTTGGGCCAAGTCAAAGGTACCGGCTTGAAAGGCCGCATCGTGGGCGACGACATCAAAGCCTTTGTGAA
ATCCGTAATGCAGGGTGGCGCGGCAAAACCTGCCGCAGCCGGCGCATCTTTGGGCAGCGGTCTGGACTTGCTGCCGTGGC
CTAAAGTGGACTTCTCCAAATTCGGCAATGTCGAAGTTAAAGAATTGTCCCGCATTAAGAAAATCTCCGGTCAAAACCTG
TCTCGCAACTGGGTTGTGATTCCGCACGTTACCGTACACGAAGAAGCGGATATGACCGAGCTGGAAGAATTCCGCAAACA
ACTGAACAAAGAATGGGAACGCGAAGGCGTGAAACTGTCTCCTCTGGCGTTCATCATCAAAGCCTCCGTTTCCGCGCTGA
AAGCCTTCCCCGAATTCAACGCTTCTTTGGACGGCGACAACCTGGTGCTGAAAAACTACTTCAACATCGGTTTCGCAGCC
GATACGCCGAACGGCTTGGTTGTTCCCGTCATCAAAGACGTGGATCAAAAAGGCTTGAAACAAATCAGCCAAGAGCTGAC
CGAATTGTCTAAAAAAGCCCGCGAAGGCAAGCTCAAACCGCAAGAAATGCAAGGCGCGTGCTTTACCATTTCCAGCTTGG
GCGGTATCGGCGGCACAGGCTTCACGCCGATTGTGAACGCTCCCGAAGTCGCCATCTTGGGCGTGTGCAAATCCCAAATC
AAACCGGTTTGGAACGGCAAAGAGTTTGCCCCGCGCCTGATGTGCCCGTTGAGCCTGTCCTTCGACCACCGCGTCATCGA
CGGTGCGGCCGGTATGCGCTTCACCGTATTCTTGGCGAAGCTATTGAAAGACTTCCGCCGCATTACCCTGTAA

Upstream 100 bases:

>100_bases
AAAACCGAATGCCCGAATGGTTTGAGCAGACAAACCGTACCGATGCCGCCTGAAGCAGCTTTCAGACGGCATCCAACTGA
ACAAGATTAAAGGAACTCAA

Downstream 100 bases:

>100_bases
CCCGATCGGCTTCAGACGGCATAGCGGTCTGTGCCGTCTGAAAAGGAAGTTTGAACATCATTCAGAAAGATGAGACATGA
GCTTAGTTGAATTGAAAGTG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKATMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGA
AAAEAAPAAAEAQPAPAAAPVAAGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
KAVFLKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAAAKIDEAAFAKAHAGPSARKLARE
LGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAKPAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNL
SRNWVVIPHVTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAA
DTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQI
KPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFLAKLLKDFRRITL

Sequences:

>Translated_530_residues
MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKATMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGA
AAAEAAPAAAEAQPAPAAAPVAAGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
KAVFLKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAAAKIDEAAFAKAHAGPSARKLARE
LGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAKPAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNL
SRNWVVIPHVTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAA
DTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQI
KPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFLAKLLKDFRRITL
>Mature_529_residues
SIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKATMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAA
AAEAAPAAAEAQPAPAAAPVAAGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVK
AVFLKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAAAKIDEAAFAKAHAGPSARKLAREL
GVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAKPAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLS
RNWVVIPHVTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAAD
TPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIK
PVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFLAKLLKDFRRITL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=555, Percent_Identity=31.7117117117117, Blast_Score=176, Evalue=7e-44,
Organism=Homo sapiens, GI110671329, Length=455, Percent_Identity=30.1098901098901, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI203098816, Length=492, Percent_Identity=26.0162601626016, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=33.7719298245614, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI203098753, Length=431, Percent_Identity=26.2180974477958, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI260898739, Length=146, Percent_Identity=36.986301369863, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786305, Length=531, Percent_Identity=54.9905838041431, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1786946, Length=429, Percent_Identity=31.9347319347319, Blast_Score=199, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17537937, Length=421, Percent_Identity=30.4038004750594, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=446, Percent_Identity=32.0627802690583, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=413, Percent_Identity=30.7506053268765, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17538894, Length=322, Percent_Identity=28.8819875776398, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=403, Percent_Identity=29.5285359801489, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324258, Length=424, Percent_Identity=29.4811320754717, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=30.3944315545244, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24645909, Length=186, Percent_Identity=37.6344086021505, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24582497, Length=431, Percent_Identity=28.0742459396752, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20129315, Length=203, Percent_Identity=32.512315270936, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 54795; Mature: 54664

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKATMDVPADAAGVVKEVK
CCEEEEECCCCCCCCCEEEEEEEEECCCEEEECCEEEEEECCCCEECCCCCHHHHHHHHH
VKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVAAGGATVQVAVPDIGGHTD
HHHCCEECCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCCCCC
VDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAVFLKVGDKVSEGSAIIEV
EEEEEEEEEECCEECCCCEEEEEECCCCEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
ETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAAAKIDEAAFAKAHAGPSARKLARE
EECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAKPAAAGASLGSGLDLLPWPKVDFSK
HCCCCHHCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
FGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEADMTELEEFRKQLNKEWEREGVKLS
CCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEC
PLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLK
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECEEEECCCCCCEEEHHHHHCCHHHHH
QISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQI
HHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHC
KPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFLAKLLKDFRRITL
CCCCCCCCCCCCEECCEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKATMDVPADAAGVVKEVK
CEEEEECCCCCCCCCEEEEEEEEECCCEEEECCEEEEEECCCCEECCCCCHHHHHHHHH
VKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVAAGGATVQVAVPDIGGHTD
HHHCCEECCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCCCCC
VDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAVFLKVGDKVSEGSAIIEV
EEEEEEEEEECCEECCCCEEEEEECCCCEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
ETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAAAKIDEAAFAKAHAGPSARKLARE
EECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAKPAAAGASLGSGLDLLPWPKVDFSK
HCCCCHHCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
FGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEADMTELEEFRKQLNKEWEREGVKLS
CCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEC
PLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLK
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECEEEECCCCCCEEEHHHHHCCHHHHH
QISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQI
HHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHC
KPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFLAKLLKDFRRITL
CCCCCCCCCCCCEECCEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]