Click here to switch to the map view.

The map label for this gene is rlmN [H]

Identifier: 161870181

GI number: 161870181

Start: 1214160

End: 1215254

Strand: Direct

Name: rlmN [H]

Synonym: NMCC_1221

Alternate gene names: 161870181

Gene position: 1214160-1215254 (Clockwise)

Preceding gene: 161870180

Following gene: 161870182

Centisome position: 56.38

GC content: 51.6

Gene sequence:

>1095_bases
ATGAAAACCAATCTGCTCAACTACGACCTTCAAGGGCTGACCCGACATTTTGCCGATACGGGCGAAAAACCCTTCCGTGC
CAAACAGGTTATGCGTTGGATGCACCAATCCGGCGCGCAAAATTTTAACGAAATGACCGATTTGGCAAAATCGTTGCGCC
ATAAACTAAATGAACAGGCAAGCATCGAAATTCCCAAGCTGATGATGTCTCAAGAATCTTCAGACGGCACTCGAAAATGG
CTACTAGATGTCGGGACGGGGAACGGGGTGGAAACCGTCTTCATCCCCGAATCGGATCGCGGCACGCTCTGCATTTCCTC
GCAAGTCGGCTGCGCTTTGGAATGTACATTTTGCTCGACCGGCCGGCAGGGCTTCAACCGCAACCTGACTGCTGCCGAAA
TCATCGGACAGTTGTGGTGGGCAAACAAAGCGATGGGGGTTACACCGAAAAACGAACGCGTAATTTCCAATGTAGTGATG
ATGGGTATGGGCGAGCCGATGGCGAACTTCGACAATGTCGTTACCGCCTTAAGCATCATGCTGGACGACCACGGCTACGG
TTTGAGCCGCCGCCGTGTAACCGTTTCCACTTCGGGTATGGTTCCCCAAATGGACAGGTTGCGCGATGTCATGCCGGTGG
CTTTGGCGGTTTCCCTCCACGCTTCCAATGACGAAGTCCGCAACCAAATCGTACCGTTGAACAAAAAATATCCCTTGAAA
GAATTGATGGCCGCATGCCAACGCTATCTGGTCAAAGCACCCAGGGATTTCATCACTTTCGAATACGTCATGTTGGACGG
AATAAACGATAGGGCGCAACATGCGCGCGAACTGATCGAACTAGTCAAAGATGTTCCCTGCAAGTTCAATCTGATTCCGT
TCAATCCCTTCCCAAACTCCGGATACGAACGCTCCAGCAATGAGAACATCCGTGTGTTCCGCGATATTTTGCAGCAGGCA
GGATTTGTCGTTACCGTACGAAAAACGCGCGGCGACGACATCGATGCCGCCTGCGGACAGTTGGCAGGTCAGGTTCAGGA
TAAAACGCGCCGCCAACAAAAATGGCAGCAGATTTTAATCGGACAACAGGGGTAA

Upstream 100 bases:

>100_bases
TCCATGCCGTCTGAAAACTCTGTTGCAAAAGGCTTCAAATCAAACTTGCCTGCCCTGCAATTTTTTATTTGAAGCCTTGA
TTTAAGAAAAACACAAACAC

Downstream 100 bases:

>100_bases
TTATGCCTTTTAAGCCATCCAAACGAATCTCTTTATTACTTGTCCTTGCCTTGGGCGCGTGCAGCACTTCCTACCGCCCC
TCGCGGGCAGAAAAAGCCAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKTNLLNYDLQGLTRHFADTGEKPFRAKQVMRWMHQSGAQNFNEMTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKW
LLDVGTGNGVETVFIPESDRGTLCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVSLHASNDEVRNQIVPLNKKYPLK
ELMAACQRYLVKAPRDFITFEYVMLDGINDRAQHARELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQA
GFVVTVRKTRGDDIDAACGQLAGQVQDKTRRQQKWQQILIGQQG

Sequences:

>Translated_364_residues
MKTNLLNYDLQGLTRHFADTGEKPFRAKQVMRWMHQSGAQNFNEMTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKW
LLDVGTGNGVETVFIPESDRGTLCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVSLHASNDEVRNQIVPLNKKYPLK
ELMAACQRYLVKAPRDFITFEYVMLDGINDRAQHARELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQA
GFVVTVRKTRGDDIDAACGQLAGQVQDKTRRQQKWQQILIGQQG
>Mature_364_residues
MKTNLLNYDLQGLTRHFADTGEKPFRAKQVMRWMHQSGAQNFNEMTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKW
LLDVGTGNGVETVFIPESDRGTLCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVSLHASNDEVRNQIVPLNKKYPLK
ELMAACQRYLVKAPRDFITFEYVMLDGINDRAQHARELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQA
GFVVTVRKTRGDDIDAACGQLAGQVQDKTRRQQKWQQILIGQQG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=353, Percent_Identity=59.4900849858357, Blast_Score=429, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 40991; Mature: 40991

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNLLNYDLQGLTRHFADTGEKPFRAKQVMRWMHQSGAQNFNEMTDLAKSLRHKLNEQA
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
SIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGTLCISSQVGCALECTFCST
CCCHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCEEEEEEECC
GRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVMMGMGEPMANFDNVVTALSIM
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVSLHASNDEVRNQIVPLNKKYPLK
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHCCCCCCCCCHH
ELMAACQRYLVKAPRDFITFEYVMLDGINDRAQHARELIELVKDVPCKFNLIPFNPFPNS
HHHHHHHHHHHHCCHHHHEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
GYERSSNENIRVFRDILQQAGFVVTVRKTRGDDIDAACGQLAGQVQDKTRRQQKWQQILI
CCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQQG
CCCC
>Mature Secondary Structure
MKTNLLNYDLQGLTRHFADTGEKPFRAKQVMRWMHQSGAQNFNEMTDLAKSLRHKLNEQA
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
SIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGTLCISSQVGCALECTFCST
CCCHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCEEEEEEECC
GRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVMMGMGEPMANFDNVVTALSIM
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVSLHASNDEVRNQIVPLNKKYPLK
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHCCCCCCCCCHH
ELMAACQRYLVKAPRDFITFEYVMLDGINDRAQHARELIELVKDVPCKFNLIPFNPFPNS
HHHHHHHHHHHHCCHHHHEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
GYERSSNENIRVFRDILQQAGFVVTVRKTRGDDIDAACGQLAGQVQDKTRRQQKWQQILI
CCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQQG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA