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The map label for this gene is rsuA [H]

Identifier: 161870172

GI number: 161870172

Start: 1203217

End: 1203987

Strand: Direct

Name: rsuA [H]

Synonym: NMCC_1211

Alternate gene names: 161870172

Gene position: 1203217-1203987 (Clockwise)

Preceding gene: 161870171

Following gene: 161870173

Centisome position: 55.87

GC content: 50.58

Gene sequence:

>771_bases
TTGGAAGCTGATTCGGATACGGCACATGACAGGACTTTCTCAGTCCTGTCTTTTTCATCCCACATTTCCCATACCATCAT
GAAACTTATCAAATACCTGCAATATCAAGGCATAGGAAGCCGCAAGCAGTGCCAATGGCTGATTGCCGGCGGTTATGTTT
TCATCAACGGAACCTGCATGGACGACACCGATGCAGACATCGATTCCTCATCCGTCGAAACGTTGGATATTGACGGGGAA
GCAGTAACCGTCGTTCCCGAACCCTATTTCTACATCATGCTCAACAAGCCTGAAGATTACGAAACTTCGCACAAACCCAA
GCACTACCGCAGCGTATTCAGCCTGTTCCCCGACAATATGCGGAACATCGATATGCAGGCGGTCGGCAGGCTGGATGCGG
ATACGACCGGCGTATTGCTGATTACCAACGACGGCAAACTGAACCACAACCTGACTTCGCCGAGCAGAAAAATTCCCAAG
CTATACGAAGTAACGCTCAAACACCCCACAGGAGAAACGCTCTGCGAAACCTTGAAAAACGGCGTGCTGCTCCACGACGA
AAACGAAACCGTTTGTGCCGCCGATGCCGTTTTGAAAAACCCGACCACCCTGCTGCTGACCATTACCGAAGGAAAATACC
ACCAAGTCAAACGCATGATCGCCGCCGCCGGCAACCGCGTGCAACACCTTCATCGCCGGCGATTCGCACATCTGGAAACA
GAAAACCTCAAACCCGGGGAATGGAAATTTATCGAATGTCCAAAATTCTGA

Upstream 100 bases:

>100_bases
TTCAAAAGTATCGCCGCGCGCTTCAATATCCATATCGACGACATCCGCCGGCTCAATCCCAACCTGAACACCATCAATCC
GGGACAGAGGGTCAAACTGA

Downstream 100 bases:

>100_bases
AATAACATCCAAGAATTCCATTTATATTCATCCACATACTCATTAAATATATGGTTTAACCCAATTTAACGCAAAAAATC
AATTTACGATATAATCCATT

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MEADSDTAHDRTFSVLSFSSHISHTIMKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSSSVETLDIDGE
AVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAVGRLDADTTGVLLITNDGKLNHNLTSPSRKIPK
LYEVTLKHPTGETLCETLKNGVLLHDENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLET
ENLKPGEWKFIECPKF

Sequences:

>Translated_256_residues
MEADSDTAHDRTFSVLSFSSHISHTIMKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSSSVETLDIDGE
AVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAVGRLDADTTGVLLITNDGKLNHNLTSPSRKIPK
LYEVTLKHPTGETLCETLKNGVLLHDENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLET
ENLKPGEWKFIECPKF
>Mature_256_residues
MEADSDTAHDRTFSVLSFSSHISHTIMKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSSSVETLDIDGE
AVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAVGRLDADTTGVLLITNDGKLNHNLTSPSRKIPK
LYEVTLKHPTGETLCETLKNGVLLHDENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLET
ENLKPGEWKFIECPKF

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=225, Percent_Identity=36.8888888888889, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787524, Length=229, Percent_Identity=29.6943231441048, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI87081838, Length=183, Percent_Identity=30.6010928961749, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790453, Length=231, Percent_Identity=25.1082251082251, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 28970; Mature: 28970

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEADSDTAHDRTFSVLSFSSHISHTIMKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCM
CCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEEEEC
DDTDADIDSSSVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNM
CCCCCCCCCCCCEEEEECCCEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCC
RNIDMQAVGRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKN
CCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHC
GVLLHDENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLET
CEEEECCCCCEEEHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
ENLKPGEWKFIECPKF
CCCCCCCEEEEECCCC
>Mature Secondary Structure
MEADSDTAHDRTFSVLSFSSHISHTIMKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCM
CCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEEEEC
DDTDADIDSSSVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNM
CCCCCCCCCCCCEEEEECCCEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCC
RNIDMQAVGRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKN
CCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHC
GVLLHDENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLET
CEEEECCCCCEEEHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
ENLKPGEWKFIECPKF
CCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA