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The map label for this gene is mltD [H]

Identifier: 161870171

GI number: 161870171

Start: 1201251

End: 1203227

Strand: Direct

Name: mltD [H]

Synonym: NMCC_1210

Alternate gene names: 161870171

Gene position: 1201251-1203227 (Clockwise)

Preceding gene: 161870167

Following gene: 161870172

Centisome position: 55.78

GC content: 56.4

Gene sequence:

>1977_bases
ATGTCCAAACTCAAAACCATCGCTCTGACCGCATCAGGTCTGTCCGTTTGTCCGGGTTTCCTATACGCCCAAAACACCTC
ATCACACCAAATCGGTTTGGCGATTATGCGCTTAAACTCTTCAATACTCGACCTGCCCCCGACAAAACAATATTTCCAAT
CCGGCAGCCTGTGGAGCGAGCTGCGCCAAGGCTTCCGGATGGGCGAAGTCAATCCCGAACTGGTACGCCGCCACGAAAGC
AAATTCATCGCAAGCCACAGCTATTTCAACAGGGTCATCAACCGGAGTAGACCCTATATGTACCATATCGCCAACGAAGT
CAAAAAACGCAATATGCCCGCCGAAGCCGCCCTGCTTCCCTTCATCGAAAGCGCGTTCGTCACCAAAGCCAAATCACACG
TCGGCGCATCAGGATTATGGCAGTTTATGCCCGCTACCGGCAGGCATTACGGCCTGGAAAAAACACCGGTTTACGACGGC
AGGCACGACGTTTACGCCGCCACCGATGCCGCACTCAACTATCTGCAATACCTCTATGGACTGTTCGGCGACTGGCCGCT
TGCCTTTGCCGCCTACAACTGGGGTGAAGGCAACGTCGGACGCGCCATCAACCGCGCCCGCGCCCAAGGGCTCGAACCGA
CCTACGAAAACCTGCGTATGCCCAACGAAACGCGCAACTATGTCCCCAAGCTGCTCGCCGTGCGCAACATCATTGCCACT
CCCCAATCTTTCGGCATGAATATCAGCGACATAGACAACAAACCCTATTTTCAGGCAGTCGAACCGGATCGTCCGCTCGA
CAACGAAGCCATCGCCCGGCTTGCCGGCATCACGCAAAGCGAGCTGCTCGCCCTAAACCCCGCATTCAACGTCCCCGCGT
TTATCCCCAAAAGCAAACGCAAACTGCTGCTTCCTGTCGCGTCCGTACAAACCTTCCAAAGCAACTACCTCAACGCCGCA
CCCGACAGCCTGTTTTCATGGGAAGTCTATACACCTGCCGCCAAAACCAGCCTGTCCGACATCTCGACGGCAACCGGCAT
GAGCATTGCCGACATCAAACGCCTCAACAACCTGAACGGCAACCTTGTCAACGCAGGACGCAGCATCCTTGTCGCCAAGA
ACGGCAAAACCCTTCAGACGGCATCGGAATCCGTCGTTTCCATCGACATCGACAATACACCCGACACCTACCGTTCCAAT
ATGCCGGCAGGCACGGTGAACGTCGGCATTGCCCGAATCCGACCCGCCGCCGCACAGACAGCGGACATTACCGTCGCACC
TTTGCCGCAGAAAACCGTCCGTACGGAACCCGATCCCCTTGTCCGTATTGCCGAACCTGCCCTTGCGACAGCCGCAGCGC
AACCTCAAACCGAAAAACAGACCGCCATGCCGTCTGAAACCCAAACCGCAACACTCGCGCAGATCGTCCCCCAAAACGAC
ATGCAGGCGGCAGACGAACTCATGCAGCTTGTTGCCCGAAACAACCTGCGCCGACAGGCTGAAGAAACCATCTCCGCCGT
CATCGGCACGCCTGACACAGTTGCCGAACACAACATTTCCTCATCTCCGCAACATACCGTTGCCGCCGACGGCAAACGCC
GGGTACGTTTGGAAACGCGCGTAGCCAAAGCTGCCGACGGCGAAGCCGAAATCTCCCCGCTCCATGCCAGCATCCACCGC
GTTGTAGAAGGCGACACCCTGTTCAACATTGCCAAACGCTACAACGTCAGTGTAGCCGACCTGATTGTCGCCAACAACAT
CAAAGGCAACACCATCCAAAAAGGACAGGTACTCCGCCTGGCGCAGGCAGCCCCTGCCCAAACCCGTATTGAAAAAGTAT
CCTACACCGCGCGCAAAGGCGACACCTTCAAAAGTATCGCCGCGCGCTTCAATATCCATATCGACGACATCCGCCGGCTC
AATCCCAACCTGAACACCATCAATCCGGGACAGAGGGTCAAACTGATTGGAAGCTGA

Upstream 100 bases:

>100_bases
CATTTCCGAATCCGCATAAAGTTCCTGTAAAACTTGACGCTTTTTCAGTCAAACAGTACCATCGGACGATAAAATATGTT
TATTCCGCCGAATATAAATC

Downstream 100 bases:

>100_bases
TTCGGATACGGCACATGACAGGACTTTCTCAGTCCTGTCTTTTTCATCCCACATTTCCCATACCATCATGAAACTTATCA
AATACCTGCAATATCAAGGC

Product: membrane bound murein transglycosylase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILDLPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHES
KFIASHSYFNRVINRSRPYMYHIANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENLRMPNETRNYVPKLLAVRNIIAT
PQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAGITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAA
PDSLFSWEVYTPAAKTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDNTPDTYRSN
MPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALATAAAQPQTEKQTAMPSETQTATLAQIVPQND
MQAADELMQLVARNNLRRQAEETISAVIGTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHR
VVEGDTLFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFKSIAARFNIHIDDIRRL
NPNLNTINPGQRVKLIGS

Sequences:

>Translated_658_residues
MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILDLPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHES
KFIASHSYFNRVINRSRPYMYHIANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENLRMPNETRNYVPKLLAVRNIIAT
PQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAGITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAA
PDSLFSWEVYTPAAKTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDNTPDTYRSN
MPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALATAAAQPQTEKQTAMPSETQTATLAQIVPQND
MQAADELMQLVARNNLRRQAEETISAVIGTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHR
VVEGDTLFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFKSIAARFNIHIDDIRRL
NPNLNTINPGQRVKLIGS
>Mature_657_residues
SKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILDLPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESK
FIASHSYFNRVINRSRPYMYHIANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDGR
HDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENLRMPNETRNYVPKLLAVRNIIATP
QSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAGITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAP
DSLFSWEVYTPAAKTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDNTPDTYRSNM
PAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALATAAAQPQTEKQTAMPSETQTATLAQIVPQNDM
QAADELMQLVARNNLRRQAEETISAVIGTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHRV
VEGDTLFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFKSIAARFNIHIDDIRRLN
PNLNTINPGQRVKLIGS

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=331, Percent_Identity=35.9516616314199, Blast_Score=211, Evalue=1e-55,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 72100; Mature: 71969

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILDLPPTKQYFQSGSLWSE
CCCCEEEEEEECCHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCHHHH
LRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYHIANEVKKRNMPAEAALLP
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHHHHH
FIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDGRHDVYAATDAALNYLQYLYG
HHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
LFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENLRMPNETRNYVPKLLAVRNIIAT
HHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCC
PQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAGITQSELLALNPAFNVPAFIPKSKR
CHHHCCCHHCCCCCCCEEECCCCCCCCHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCCC
KLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAAKTSLSDISTATGMSIADIKRLNNLNG
EEEEEHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCC
NLVNAGRSILVAKNGKTLQTASESVVSIDIDNTPDTYRSNMPAGTVNVGIARIRPAAAQT
CEEECCCEEEEECCCCEEECCCCCEEEEECCCCCCHHHCCCCCCCEEECHHHCCCCCCCC
ADITVAPLPQKTVRTEPDPLVRIAEPALATAAAQPQTEKQTAMPSETQTATLAQIVPQND
CCEEEECCCHHHHCCCCCCCEEECCHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHCCCCH
MQAADELMQLVARNNLRRQAEETISAVIGTPDTVAEHNISSSPQHTVAADGKRRVRLETR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEECCCCCEEEEHHH
VAKAADGEAEISPLHASIHRVVEGDTLFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRL
HHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHEEEECCCCCCCCCCCHHEEH
AQAAPAQTRIEKVSYTARKGDTFKSIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS
HHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILDLPPTKQYFQSGSLWSE
CCCEEEEEEECCHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCHHHH
LRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYHIANEVKKRNMPAEAALLP
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHHHHH
FIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDGRHDVYAATDAALNYLQYLYG
HHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
LFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENLRMPNETRNYVPKLLAVRNIIAT
HHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCC
PQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAGITQSELLALNPAFNVPAFIPKSKR
CHHHCCCHHCCCCCCCEEECCCCCCCCHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCCC
KLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAAKTSLSDISTATGMSIADIKRLNNLNG
EEEEEHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCC
NLVNAGRSILVAKNGKTLQTASESVVSIDIDNTPDTYRSNMPAGTVNVGIARIRPAAAQT
CEEECCCEEEEECCCCEEECCCCCEEEEECCCCCCHHHCCCCCCCEEECHHHCCCCCCCC
ADITVAPLPQKTVRTEPDPLVRIAEPALATAAAQPQTEKQTAMPSETQTATLAQIVPQND
CCEEEECCCHHHHCCCCCCCEEECCHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHCCCCH
MQAADELMQLVARNNLRRQAEETISAVIGTPDTVAEHNISSSPQHTVAADGKRRVRLETR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEECCCCCEEEEHHH
VAKAADGEAEISPLHASIHRVVEGDTLFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRL
HHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHEEEECCCCCCCCCCCHHEEH
AQAAPAQTRIEKVSYTARKGDTFKSIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS
HHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]