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The map label for this gene is ssoIIM [H]

Identifier: 161870164

GI number: 161870164

Start: 1194172

End: 1195185

Strand: Reverse

Name: ssoIIM [H]

Synonym: NMCC_1203

Alternate gene names: 161870164

Gene position: 1195185-1194172 (Counterclockwise)

Preceding gene: 161870165

Following gene: 161870163

Centisome position: 55.5

GC content: 32.94

Gene sequence:

>1014_bases
ATGAAAAATAAAATACAAAATCAATTAGATAATATAGTTACCTTAAATAATGAGAAATTTACTTTTATTGATTTATTTGC
GGGAATAGGTGGTTTTCGCATTGCAATGGAGAATGTTGGTGGGCGATGTGTATTTTCTAGTGAATGGGATGATAAAGCCC
GTCAAACCTACCAAGTAAATTTTAATGATATTCCTTATGGAGATATTACATTAAAGGAAACCAAAGCAGCTATTCCAAGT
AAGTTTGATGTATTAACAGCAGGATTCCCGTGTCAGCCATTCTCTATAGCTGGTGTTTCAAAGAAAAAAAGCCTAGGACG
AGAAACAGGCTTCTTAGATAAGGCGCAGGGAACTCTATTTTTTGATGTTGCTGAAATTATTGGAAAACATCGACCTAAAA
TTTTTCTTTTAGAGAATGTGAAAAACCTTGTTTCGCATGACAAAGGAAATACATTTAAAGTAATTAAAGGGACTTTAGAA
GAGCTTGACTATCAGATATTTTATCAAGTTATGAATGCAAAATATTATGTTCCTCAAAATAGGGAGCGTATTTTTATTGT
AGGTTTTGATAGACAATATTTTAATAAGGAAATAAATTTCAATTTTCCTTCCCCACCAGAATCACAACCAAAATTAAAGC
AAATTTTGGAAGATGATGTAGATAATTCTTTTACTCTTTCTGATAACTTATGGCTTTACCTTCAAAATTACGCTAAAAAA
CATAAGGCAAAGGGTAATGGATTTGGTTTTGGATTAGTTGATTTAGATGGAATATCACGAACTCTATCTGCACGATATTA
CAAAGATGGTTCCGAAATACTCATCCCTCAGAAAGGAAAAAATCCTAGGAAGCTGACACCTAGGGAGTGCTCGCGTTTAA
TGGGATTTCCTAAAGATTTTGTTATTGATGCAGTATCCAAGACAGCTGCATACAAGCAGTTTGGCAATTCAATTGCTGTA
CCGTTGGTTCAAGCTATTGCTAAACAAATTATAAATGAGTTAAAAAATGAATGA

Upstream 100 bases:

>100_bases
TATTCAGGATGACGGCTGCGCGGGCGGGGCTTGTAAGATTTGATGAAAGGGGGAGTTTTCAGATGGCCTTTAGATTAATA
AATCATCTGAAATATAAAAT

Downstream 100 bases:

>100_bases
CTTGGCAGCTCAGACAATACAAGTAGTAAATAAATCCGATGACTTAGTAGCTTCAGCAATACAGACAGCAAATAAATCAA
TTGCAGTATACTGTCGTTAT

Product: C-5 cytosine-specific DNA-methylase

Products: NA

Alternate protein names: M.SsoII; Cytosine-specific methyltransferase SsoII [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCVFSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPS
KFDVLTAGFPCQPFSIAGVSKKKSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILEDDVDNSFTLSDNLWLYLQNYAKK
HKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIPQKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAV
PLVQAIAKQIINELKNE

Sequences:

>Translated_337_residues
MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCVFSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPS
KFDVLTAGFPCQPFSIAGVSKKKSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILEDDVDNSFTLSDNLWLYLQNYAKK
HKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIPQKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAV
PLVQAIAKQIINELKNE
>Mature_337_residues
MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCVFSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPS
KFDVLTAGFPCQPFSIAGVSKKKSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILEDDVDNSFTLSDNLWLYLQNYAKK
HKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIPQKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAV
PLVQAIAKQIINELKNE

Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SsoII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=354, Percent_Identity=46.6101694915254, Blast_Score=285, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF00145 DNA_methylase; PF01381 HTH_3 [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 38291; Mature: 38291

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCVFSSEWDDKARQTYQVN
CCCHHHHHHCCEEEECCCCEEEEEHHHCCCCEEEEEECCCCEEEECCCCCCCCCEEEEEE
FNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKKKSLGRETGFLDKAQGTLF
CCCCCCCCEEEHHHHHCCCCCCCEEECCCCCCCCEECCCCHHHHCCHHCCCCCCCCCCEE
FDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLEELDYQIFYQVMNAKYYVPQN
EEHHHHHHCCCCEEEEHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCEEECCCC
RERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILEDDVDNSFTLSDNLWLYLQNYAKK
CCEEEEEECCHHHHCCEECCCCCCCCCCCHHHHHHHHHCCCCCEEECCCEEEHHHHHHHH
HKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIPQKGKNPRKLTPRECSRLMGFPKDF
HCCCCCCCEEEEEECCCHHHHHHHHEECCCCEEEEECCCCCCCCCCHHHHHHHHCCCHHH
VIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINELKNE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCVFSSEWDDKARQTYQVN
CCCHHHHHHCCEEEECCCCEEEEEHHHCCCCEEEEEECCCCEEEECCCCCCCCCEEEEEE
FNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKKKSLGRETGFLDKAQGTLF
CCCCCCCCEEEHHHHHCCCCCCCEEECCCCCCCCEECCCCHHHHCCHHCCCCCCCCCCEE
FDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLEELDYQIFYQVMNAKYYVPQN
EEHHHHHHCCCCEEEEHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCEEECCCC
RERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILEDDVDNSFTLSDNLWLYLQNYAKK
CCEEEEEECCHHHHCCEECCCCCCCCCCCHHHHHHHHHCCCCCEEECCCEEEHHHHHHHH
HKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIPQKGKNPRKLTPRECSRLMGFPKDF
HCCCCCCCEEEEEECCCHHHHHHHHEECCCCEEEEECCCCCCCCCCHHHHHHHHCCCHHH
VIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINELKNE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7916706 [H]