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The map label for this gene is cca

Identifier: 161870084

GI number: 161870084

Start: 1109640

End: 1110893

Strand: Direct

Name: cca

Synonym: NMCC_1122

Alternate gene names: 161870084

Gene position: 1109640-1110893 (Clockwise)

Preceding gene: 161870082

Following gene: 161870085

Centisome position: 51.53

GC content: 58.37

Gene sequence:

>1254_bases
ATGCAGACTTACCTCGTCGGCGGCGCCGTCCGCGATTATCTTTTGGGCTTGCCCGTCAAAGACCGCGATTGGGTGGTCGT
CGGCGCAGACGCGCAAACCATGCTGGCGCAAGGCTTCCAGCCTGTCGGCAAAGACTTTCCCGTGTTTCTCCATCCCGAAA
CGCACGAAGAATACGCCCTCGCCCGCACCGAACGCAAAACCGCCAAAGGTTACGCCGGTTTCAGTTTCCACGCCGACAAA
GACGTTACGCTGGAGCAGGATTTGATGCGCCGCGACCTGACCATCAACGCGATGGCGCAAGATGCGGGCGGCAAGATTAT
CGACCCTTTCGGCGGACAACGGGATTTGGCGGCAGGCATTTTGCGCCACGTTTCCCCGGCCTTCGCCGAAGACCCCGTCC
GCATCCTGCGTACTGCCCGCTTTGCCGCGCGTTACAGGTTTGAAATCGCCGAAGAAACCATAAAGCTGATGCGGCAGATG
GTGGAAAACGGCGAAGCGGACGCATTGGTTGCCGAACGCGTCTGGCAGGAGTTGGCGAAAGGTTTGATGGAAAAAAATCC
GCGCAAAATGATTGAAGTGTTGCGCGAATGCGGTGCGCTCAAAGTCTTGCTGCCCGAAGTCAATGCCCTCTTCGGCGTGC
CGCAACGCGCCGACTACCATCCCGAAATCGACAGCGGCATCCATACCCTGATGACACTGCAACGCGCCGCCGATATGGGT
TTGAGCCTGCCCGAACGCTATGCCGCCCTGCTGCACGACTTGGGCAAAGCCAAAACGCCGCCCGACATCCTGCCGCGCCA
CCACGGACACGACCTCGCCGGTGTCGAACCCGTGCGCGAAGTCAATCAAAGGTTGCGCGCGCCGAAACATTGCGCCGAGC
TTGCCGAATTGGTTTGCCGTTGGCACATTATTTTCCACCAAGTCGGACAGCTTAAAAGCCAAACCATTCTGAACGTTTTG
AAAAAAACCGACGCTTTCAGACGACCCGAACGCTTTCAGACGGCATTGAACGTCTGCATTGCCGACACGCAAGGCCGTCT
GAACCGCGAACACACGCCCTACCCGCAACGCGCGCACTGGCTCGCCTTACTCGAAGCCGCCAATCAGGCGGATTCGGGCA
AAATCGCCGCCGAATGCCGGGCGCAAGGAAAAGCGCACCTTATCGCCGAACAAATCGACCGGATGCGGCTGGCACAAATC
GCCCCGCTGCAAAAAGCGTTTCAAGCGGCGCAAGACAAAACAGAAAAACATTAA

Upstream 100 bases:

>100_bases
AAAAGGCATAGCATAAGCGCACAAAATACCCGTCAGTTGAAACAATGCCGTCTGAAGCCGCTTCAGACGGCATTATTGAT
AACTTTAAAATAGGAAGCAT

Downstream 100 bases:

>100_bases
AACGCCCAATGCAGCCACTTTTATAGTGGATTAACAAAAATCAGGACAAGGCGACGAAGCCGCAGACAGTACAAATAGTA
CGGCAAGGCGAGGCAACGCT

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGKDFPVFLHPETHEEYALARTERKTAKGYAGFSFHADK
DVTLEQDLMRRDLTINAMAQDAGGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
VENGEADALVAERVWQELAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRADYHPEIDSGIHTLMTLQRAADMG
LSLPERYAALLHDLGKAKTPPDILPRHHGHDLAGVEPVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVL
KKTDAFRRPERFQTALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQIDRMRLAQI
APLQKAFQAAQDKTEKH

Sequences:

>Translated_417_residues
MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGKDFPVFLHPETHEEYALARTERKTAKGYAGFSFHADK
DVTLEQDLMRRDLTINAMAQDAGGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
VENGEADALVAERVWQELAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRADYHPEIDSGIHTLMTLQRAADMG
LSLPERYAALLHDLGKAKTPPDILPRHHGHDLAGVEPVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVL
KKTDAFRRPERFQTALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQIDRMRLAQI
APLQKAFQAAQDKTEKH
>Mature_417_residues
MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGKDFPVFLHPETHEEYALARTERKTAKGYAGFSFHADK
DVTLEQDLMRRDLTINAMAQDAGGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
VENGEADALVAERVWQELAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRADYHPEIDSGIHTLMTLQRAADMG
LSLPERYAALLHDLGKAKTPPDILPRHHGHDLAGVEPVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVL
KKTDAFRRPERFQTALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQIDRMRLAQI
APLQKAFQAAQDKTEKH

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=205, Percent_Identity=30.2439024390244, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789436, Length=404, Percent_Identity=54.7029702970297, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI87081691, Length=169, Percent_Identity=33.7278106508876, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_NEIM0 (A9LZC1)

Other databases:

- EMBL:   CP000381
- RefSeq:   YP_001599254.1
- ProteinModelPortal:   A9LZC1
- SMR:   A9LZC1
- EnsemblBacteria:   EBNEIT00000012891
- GeneID:   5795336
- GenomeReviews:   CP000381_GR
- KEGG:   nmn:NMCC_1122
- GeneTree:   EBGT00050000020819
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- ProtClustDB:   PRK10885
- BioCyc:   NMEN374833:NMCC_1122-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46826; Mature: 46826

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGKDFPVFLHPETHEEYAL
CCCEEEHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDAGGKIIDPFGGQRDLAAGI
HHHHHHHHCCCCCCEECCCCCCCHHHHHHHHCCCCEEHHHCCCCCEECCCCCCHHHHHHH
LRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQMVENGEADALVAERVWQELAK
HHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRADYHPEIDSGIHTLMTLQRAADMG
HHHCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
LSLPERYAALLHDLGKAKTPPDILPRHHGHDLAGVEPVREVNQRLRAPKHCAELAELVCR
CCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
WHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQTALNVCIADTQGRLNREHTPYPQRAHW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
LALLEAANQADSGKIAAECRAQGKAHLIAEQIDRMRLAQIAPLQKAFQAAQDKTEKH
HHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGKDFPVFLHPETHEEYAL
CCCEEEHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDAGGKIIDPFGGQRDLAAGI
HHHHHHHHCCCCCCEECCCCCCCHHHHHHHHCCCCEEHHHCCCCCEECCCCCCHHHHHHH
LRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQMVENGEADALVAERVWQELAK
HHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRADYHPEIDSGIHTLMTLQRAADMG
HHHCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
LSLPERYAALLHDLGKAKTPPDILPRHHGHDLAGVEPVREVNQRLRAPKHCAELAELVCR
CCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
WHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQTALNVCIADTQGRLNREHTPYPQRAHW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
LALLEAANQADSGKIAAECRAQGKAHLIAEQIDRMRLAQIAPLQKAFQAAQDKTEKH
HHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA