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The map label for this gene is tilS [H]

Identifier: 161870026

GI number: 161870026

Start: 1040513

End: 1041823

Strand: Direct

Name: tilS [H]

Synonym: NMCC_1059

Alternate gene names: 161870026

Gene position: 1040513-1041823 (Clockwise)

Preceding gene: 161870025

Following gene: 161870027

Centisome position: 48.32

GC content: 53.39

Gene sequence:

>1311_bases
GTGCTGACTTTAGATGCGTTTGAGCAATGCTTGAAGGATTGTTTCCCTCAAGGTCTGAATGGAAAAAAAACAGCAGTGGC
ATTAAGCGGCGGCTTGGATTCCGTGGTCTTGCTGCACCTGCTTGTCCGCGCTGGGAAACGGGCAGGATTTGTGCCGGAGG
CATTGCATATCCATCACGGCTTGAGCCCCCGTGCCGACGATTGGGCGGATTTCTGCCAAAATTATTGCGATATGCTCGGG
GTGGGGCTGGAAACGGTTAAGGTCTGCGTGGAAAAAAACGGTTTGGGCATCGAGGCGGCGGCGCGGCAAAAGCGTTATGC
CGCGTTTGCCGAAAAAGGCTTTGACGTTTTGGCGTTGGCGCACCACAGGGACGATCAAATCGAAACCTTTATGCTGGCAG
TCGCTCGCGGCGGCGGTTTGCGCGCTTTGGCGGCTATGCCCACCGTCCGCCCTTTTGGGGAAAAAGGCATCATCTGGCGG
CCTTTGCTGCCTTTTTCGCGCCAAGATATATGGGATTATGCCCAAAAACACGGTTTGCCGAATATCGAGGATGAAAGCAA
TAGCGATACGGCTTATTTGCGAAACCGCTTCCGGCACCGTATTTTGCCCGAACTTTCGGCGCAGATTCCCCATTTCGGGC
GGCATGTGCTGAACAATGTCCGCGCTTTGCAGGAAGATTTGGCTTTGTTGGACGAAATCATCGGTCAGGACTGCCGTTGG
GTTTGCGGAGCCGGTTATTTCGATACGGCGCGGTGGCTGACGTTTTCCCCGCGCCGGAAAACCCATATTTTGCGGCATTT
TTTGAAGGAAAACGGCATTCCCGTGCCGAATCAGAATGCCCTTGCCGACATTGCCCGGGTTTTGACGGAGGCAAAAACCG
GACGTTGGAACGTGCAAGGCTTTGAATTGCATCATTATGCAGGCAGGCTGTTTGTGTTCCGACCGGAAAAAACGGATAAA
CTGCGGTTTTTGAAAGACAGGCAGATAAGCGGAAATTTAAGGGAAATATTGGCGGGGCAGGGATTTGTTTTAAAACGTCA
TCCGTTCGGACTTCCCGGGCATCTTTTGGAGCAGGACGGAATCTTGCGGACGGTTGCGGCATCGGACAGGCTGGCTGTCG
GCGGTATCCATAAGGATGTGAAGAAAATCCTTCAGGAAAAGCGGGTTTTGCCCGCGCTGCGCCCGTTTTGGCCGGTCGTC
GCCGACAAGGACAACCGTCCGCTGGCGTTGGCAAATTACGGTACGGATTTTCAAATCTCGGTTTCAGACGGCATTTTGCC
CGTCCATCCGGATTTTCCCGTTTTATTTTGA

Upstream 100 bases:

>100_bases
CATTATGGCTTACGGCAAATTTTCGGAACAATAATTCAGGTAGAACAAGCAGCAAGCAGTTTGTCTGAAACTGCTTGCTT
TTTCTTTATCGGGGCGCGGC

Downstream 100 bases:

>100_bases
TGGAATCGGAATCGCCGTTACCTATTATATATTCTTGAAAAGGCGGTAAAGTGAAGCATATCAGTTCGGCAAACAATGAA
CACATCAGACACCTGCACCG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRAGKRAGFVPEALHIHHGLSPRADDWADFCQNYCDMLG
VGLETVKVCVEKNGLGIEAAARQKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLNNVRALQEDLALLDEIIGQDCRW
VCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVPNQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDK
LRFLKDRQISGNLREILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPALRPFWPVV
ADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF

Sequences:

>Translated_436_residues
MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRAGKRAGFVPEALHIHHGLSPRADDWADFCQNYCDMLG
VGLETVKVCVEKNGLGIEAAARQKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLNNVRALQEDLALLDEIIGQDCRW
VCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVPNQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDK
LRFLKDRQISGNLREILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPALRPFWPVV
ADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF
>Mature_436_residues
MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRAGKRAGFVPEALHIHHGLSPRADDWADFCQNYCDMLG
VGLETVKVCVEKNGLGIEAAARQKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLNNVRALQEDLALLDEIIGQDCRW
VCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVPNQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDK
LRFLKDRQISGNLREILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPALRPFWPVV
ADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=412, Percent_Identity=34.2233009708738, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 48945; Mature: 48945

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRAGKRAGFVPEALHIHHG
CCCHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
LSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAARQKRYAAFAEKGFDVLALA
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEE
HHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWRPLLPFSRQDIWDYAQKHGLP
CCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCC
NIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLNNVRALQEDLALLDEIIGQDCRW
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVPNQNALADIARVLTEAKTGRWNVQG
EECCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
FELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLREILAGQGFVLKRHPFGLPGHLLEQDG
EEEEECCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHCCCC
ILRTVAASDRLAVGGIHKDVKKILQEKRVLPALRPFWPVVADKDNRPLALANYGTDFQIS
CEEEECCCCCEEECCHHHHHHHHHHHHHHCHHHHCCCCCEECCCCCCEEEEECCCCEEEE
VSDGILPVHPDFPVLF
ECCCEEECCCCCCCCC
>Mature Secondary Structure
MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRAGKRAGFVPEALHIHHG
CCCHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
LSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAARQKRYAAFAEKGFDVLALA
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEE
HHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWRPLLPFSRQDIWDYAQKHGLP
CCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCC
NIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLNNVRALQEDLALLDEIIGQDCRW
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVPNQNALADIARVLTEAKTGRWNVQG
EECCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
FELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLREILAGQGFVLKRHPFGLPGHLLEQDG
EEEEECCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHCCCC
ILRTVAASDRLAVGGIHKDVKKILQEKRVLPALRPFWPVVADKDNRPLALANYGTDFQIS
CEEEECCCCCEEECCHHHHHHHHHHHHHHCHHHHCCCCCEECCCCCCEEEEECCCCEEEE
VSDGILPVHPDFPVLF
ECCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA