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The map label for this gene is purH [H]

Identifier: 161869895

GI number: 161869895

Start: 929724

End: 931304

Strand: Direct

Name: purH [H]

Synonym: NMCC_0926

Alternate gene names: 161869895

Gene position: 929724-931304 (Clockwise)

Preceding gene: 161869894

Following gene: 161869896

Centisome position: 43.17

GC content: 58.0

Gene sequence:

>1581_bases
ATGTCCGTCATCAAACGCGCCCTGATCAGCCTGTCCGACAAGGCAGGCGCGGTCGAATTTGCCCAAACCCTGACCAAGCT
CGGTGTTGAAATCCTCTCTACCGGCGGTACAGCAAAGCTCTTGGCTGATGCAGGCGTTCCCGTTATCGAAGTTGCCGACT
ATACCGGCTTCCCCGAAATGCTCGATGGCCGCGTGAAAACCCTGCATCCGAAAATCCACGGCGGCATCCTCGGTCGTCGC
GATTTGGGCGAACACGTCGCCAAGATGGAAGAACACGGTATCGGCAACATCGACCTCGTGTGCGTCAACCTTTACCCCTT
CGCCGCCACCATCGCCAAACCAAACTGCACACTGGAAGACGCGATTGAAAACATCGACATCGGCGGCCCGACTATGGTGC
GTTCTGCCGCGAAAAACTGGAAACACGTCGCCATCGTTACCGATACCGCCGATTTCCCTGCCATCGCCGCCGAACTCGAA
GCCAACAACGGCGCATTGAGCGACAAAACCCGTTTCAACCTCTCACGCAAAGCATTCAGCCATACCGCCCAATACGACGG
CATGATTTCCAACTACCTGACCTCGCTTTCAGACGACGTCTTGAGCGGAGAGCCCCAAATCGGCGAATTCCCGAGCCAGT
TCAACCAAAGCTGGATTAAAGTGCAAGATATGCGCTACGGCGAGAATCCGCACCAACGCGCCGCGTTCTACCGCGATGTG
TACCCCGCCGCAGGCAGCCTTTCCGCCTACAAACAGCTCCAAGGTAAAGAATTGTCGTACAACAACATCGCCGATGCCGA
TGCCGCTTGGGAAGCCGTCAAATCCTTCGACGCGCCCGCCTGCGTGATTGTGAAGCACGCCAATCCGTGCGGCGTCGCCG
TTGCAGCCGATACCTTGACCGCCTACAAACTCGCCTACGCCACCGACACCACCAGCGCGTTCGGCGGCATCATCGCCTTC
AACCGCGAAGTGGACGGCGAAACCGTCAAACAGATTACAGACAACCAGTTTATGGAAGTCCTGATGGCGCCGAAGTTCAC
CGCCGAAGCCCTCGAAATCGCCGCCGCCAAGAAAAACGTGCGCGTGTTGGAAGTGCCTTTGGAAGCAGGCGCGAACCGCT
TTGAACTCAAACGCGTCGGCGGCGGATTGTTGGTACAAACGCCCGACATCCACCGCATCAGCCGCGCCGATTTGAAAGTC
GTCTCCAAACGCCAACCGACCGAACAGGAATGGAACGATTTGCTGTTCGTTTGGAACGTCGCAAAATACGTCAAATCCAA
CGCCATCGTCTTCGGCAAAGGCGGTCAAACCTACGGCATCGGCGCAGGCCAAATGAGCCGCGTGGACAGTACCCGCATCG
CCGCCCGCAAAGCGCAGGACGCAGGCTTGGACTTAAACGGCGCATGTGCCGCCTCTGATGCTTTCTTCCCCTTCCGCGAC
GGCGTGGACGTGATTGCCGAGCAGGGCATCAAAGCCATCATCCATCCGGCAGGTTCGATGCGCGATCAGGAAGTCTTCGA
CGCAGCGGACGAACACGGCATCGCCATGGTCGTAACCGACGTGCGCCACTTCCGCCATTAA

Upstream 100 bases:

>100_bases
TATCTGCAAAAACCTACTGCCAAAAAACTAGAAATAGCGGATAATACCGCCCCGAAAAAGTTCCCCTTTCCCCATCCAAC
TGTCAGAAAAGGAAGCCCCA

Downstream 100 bases:

>100_bases
CGCAGATGAACAAGGCAATGCCGTCTGAAGGGCTTTCAGACGGCATTTTGCGCTATTTTGTGAAGGTAGGGATGACGGTT
CGGGTATTCCTGACAGGGTG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADAGVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRR
DLGEHVAKMEEHGIGNIDLVCVNLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPQIGEFPSQFNQSWIKVQDMRYGENPHQRAAFYRDV
YPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSFDAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAF
NREVDGETVKQITDNQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHRISRADLKV
VSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDSTRIAARKAQDAGLDLNGACAASDAFFPFRD
GVDVIAEQGIKAIIHPAGSMRDQEVFDAADEHGIAMVVTDVRHFRH

Sequences:

>Translated_526_residues
MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADAGVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRR
DLGEHVAKMEEHGIGNIDLVCVNLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPQIGEFPSQFNQSWIKVQDMRYGENPHQRAAFYRDV
YPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSFDAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAF
NREVDGETVKQITDNQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHRISRADLKV
VSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDSTRIAARKAQDAGLDLNGACAASDAFFPFRD
GVDVIAEQGIKAIIHPAGSMRDQEVFDAADEHGIAMVVTDVRHFRH
>Mature_525_residues
SVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADAGVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRD
LGEHVAKMEEHGIGNIDLVCVNLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELEA
NNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPQIGEFPSQFNQSWIKVQDMRYGENPHQRAAFYRDVY
PAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSFDAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFN
REVDGETVKQITDNQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHRISRADLKVV
SKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDSTRIAARKAQDAGLDLNGACAASDAFFPFRDG
VDVIAEQGIKAIIHPAGSMRDQEVFDAADEHGIAMVVTDVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=481, Percent_Identity=37.006237006237, Blast_Score=260, Evalue=3e-69,
Organism=Escherichia coli, GI1790439, Length=532, Percent_Identity=60.5263157894737, Blast_Score=633, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=488, Percent_Identity=35.0409836065574, Blast_Score=238, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI71985574, Length=337, Percent_Identity=28.7833827893175, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=46.6666666666667, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323768, Length=483, Percent_Identity=35.4037267080745, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6323056, Length=490, Percent_Identity=35.3061224489796, Blast_Score=249, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24649832, Length=495, Percent_Identity=36.969696969697, Blast_Score=259, Evalue=3e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56843; Mature: 56712

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADAGVPVIEVADYTGFPEM
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCEEEECCCCCCHHH
LDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCVNLYPFAATIAKPNCTLED
HCCCCEEECCHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHCCCCCCHHH
AIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELEANNGALSDKTRFNLSRKAFS
HHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHCHHHHHHH
HTAQYDGMISNYLTSLSDDVLSGEPQIGEFPSQFNQSWIKVQDMRYGENPHQRAAFYRDV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
YPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSFDAPACVIVKHANPCGVAVAADTLT
CCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHH
AYKLAYATDTTSAFGGIIAFNREVDGETVKQITDNQFMEVLMAPKFTAEALEIAAAKKNV
HEEEEEECCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
RVLEVPLEAGANRFELKRVGGGLLVQTPDIHRISRADLKVVSKRQPTEQEWNDLLFVWNV
EEEEEEHHCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHCEEEEEHH
AKYVKSNAIVFGKGGQTYGIGAGQMSRVDSTRIAARKAQDAGLDLNGACAASDAFFPFRD
HHHHHCCEEEECCCCCEEECCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GVDVIAEQGIKAIIHPAGSMRDQEVFDAADEHGIAMVVTDVRHFRH
CHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
SVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADAGVPVIEVADYTGFPEM
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCEEEECCCCCCHHH
LDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCVNLYPFAATIAKPNCTLED
HCCCCEEECCHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHCCCCCCHHH
AIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELEANNGALSDKTRFNLSRKAFS
HHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHCHHHHHHH
HTAQYDGMISNYLTSLSDDVLSGEPQIGEFPSQFNQSWIKVQDMRYGENPHQRAAFYRDV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
YPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSFDAPACVIVKHANPCGVAVAADTLT
CCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHH
AYKLAYATDTTSAFGGIIAFNREVDGETVKQITDNQFMEVLMAPKFTAEALEIAAAKKNV
HEEEEEECCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
RVLEVPLEAGANRFELKRVGGGLLVQTPDIHRISRADLKVVSKRQPTEQEWNDLLFVWNV
EEEEEEHHCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHCEEEEEHH
AKYVKSNAIVFGKGGQTYGIGAGQMSRVDSTRIAARKAQDAGLDLNGACAASDAFFPFRD
HHHHHCCEEEECCCCCEEECCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GVDVIAEQGIKAIIHPAGSMRDQEVFDAADEHGIAMVVTDVRHFRH
CHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]