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The map label for this gene is cysT [H]

Identifier: 161869814

GI number: 161869814

Start: 846028

End: 846864

Strand: Reverse

Name: cysT [H]

Synonym: NMCC_0843

Alternate gene names: 161869814

Gene position: 846864-846028 (Counterclockwise)

Preceding gene: 161869817

Following gene: 161869813

Centisome position: 39.33

GC content: 59.14

Gene sequence:

>837_bases
ATGTTACCGCTCAAAACGCCCGGCGTACTGCCCGGCTTCAAACTCAGCCTTGGTCTGACCGTATTGTGCCTGTCGCTGCT
TGTGGTCTTGCCGTTTGCGATGATGGCGGCGAAGGCGGCGGAAATCGGCTGGGGCGGCTTTTGGAACACGATTGCCGAGC
CGAACGTGTTGGCGGCGGTATGGCTGAGCTTGCGGATGTCGTTTTATGCGATGCTGACCAATGTCGTGTTCGGCACGCTG
GTGGCGTGGGTATTGGTGCGTTATGAATTCCCGGGCAAGGGTCTGGCGAACGCGCTGGTCGATTTGCCGTTTGCGCTGCC
GACGGCGGTTACGGGTATCGCGTTGGCGACCCTGTATGCGCCCAACGGTTGGATAGGCCGTTTTTTCGAGCCTTTGGGCA
TCAAAATCGCGTTTACGCCCGTCGGCATTTGGATTGCGCTGGTCGTCGTCAGCCTGCCCTTTATCGTCCGCGCCGTGCAG
CCGGTGTTGGAGGAATTATCGGGCGAATATGAGGAAGCGGCGGCGACTTTGGGCGCAAACCGCTTTACGACGTTCCGCCG
CGTGCTGCTGCCGGAAATCACGCCGGCACTCTTGACTGGCGCGGGAATGATGTTCGCGCGGGCAACGGGGGAATACGGCT
CGGTGATTTTTATCGCGGGCAATATTCCGATGGTTTCTGAAATCCTGCCGCTGATTATTACGGGCAAGCTGGAACAGTTC
GACGTACAGGGCGCGTCGGCGGTGGCGTTGTTTATGCTGCTGGTTTCGTTTGTGATTCTGTTTGCGCTGAACGTGATGCA
GTGGGCGTTGGGCAGGCGTTCGGGCGCGAAGGGTTGA

Upstream 100 bases:

>100_bases
TATAACAAATTCGTCTTTGCACCAAACTTTCCATTCTTTCCGTTTTTCGGACGGCATCGTTAAAGTAGTCCTTCCTTTTC
CTTATTTTCAGCATTGTTTT

Downstream 100 bases:

>100_bases
GGTCGTCTGAAATACCTGTTACCGTCATTCCCGCGCAGGCGGGAATCCATTGGTGAATTTCAGCTGCCTTATTTATTTTC
TGTTTTCCTGTTGCCCTGCG

Product: sulfate ABC transporter, permease protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGWGGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTL
VAWVLVRYEFPGKGLANALVDLPFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFIAGNIPMVSEILPLIITGKLEQF
DVQGASAVALFMLLVSFVILFALNVMQWALGRRSGAKG

Sequences:

>Translated_278_residues
MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGWGGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTL
VAWVLVRYEFPGKGLANALVDLPFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFIAGNIPMVSEILPLIITGKLEQF
DVQGASAVALFMLLVSFVILFALNVMQWALGRRSGAKG
>Mature_278_residues
MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGWGGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTL
VAWVLVRYEFPGKGLANALVDLPFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFIAGNIPMVSEILPLIITGKLEQF
DVQGASAVALFMLLVSFVILFALNVMQWALGRRSGAKG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=273, Percent_Identity=47.2527472527472, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI87082099, Length=258, Percent_Identity=32.1705426356589, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1786980, Length=173, Percent_Identity=39.8843930635838, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787369, Length=170, Percent_Identity=28.2352941176471, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786254, Length=227, Percent_Identity=24.6696035242291, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787368, Length=226, Percent_Identity=29.2035398230088, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29792; Mature: 29792

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]