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The map label for this gene is norM [H]

Identifier: 161869757

GI number: 161869757

Start: 782411

End: 783790

Strand: Reverse

Name: norM [H]

Synonym: NMCC_0777

Alternate gene names: 161869757

Gene position: 783790-782411 (Counterclockwise)

Preceding gene: 161869763

Following gene: 161869755

Centisome position: 36.4

GC content: 57.03

Gene sequence:

>1380_bases
ATGCTGCTCGACCTCAACCGCTTTTCCTTTTCCGTCTTCCTGAAAGAAGTCCGCCTGCTGACCGCTCTTGCCCTGCCCAT
GCTGTTGGCGCAGGTCGCGCAGGTGGGCATCGGTTTTGTCGATACCGTGATGGCGGGCGGTGCGGGCAAGGAAGACTTGG
CGGCGGTGGCTTTGGGCAGCAGCGCGTTTGCCACGGTTTATATTACCTTTATGGGCATTATGGCGGCGCTGAACCCGATG
ATTGCCCAGCTTTACGGCGCGGGTAAAACCGACGAAGTGGGCGAAACGGGACGGCAGGGGATTTGGTTCGGGCTGTTTTT
GGGCGTGTTCGGCATGGTCTTGATGTGGGCGGCGATTACGCCGTTCCGCAACTGGCTGACCTTGAGCGATTATGTGGAAG
GCACAATGGCGCAGTATATGCTGTTCACCAGCTTGGCGATGCCGGCGGCAATGGTACACCGCGCACTGCACGCCTACGCC
TCCAGCCTGAACCGCCCGCGCCTGATTATGTTGGTCAGCTTTGCGGCGTTTGTGTTGAACGTGCCGCTGAACTATATTTT
CGTTTACGGCAAATTCGGTATGCCCGCTTTGGGCGGCGCAGGCTGCGGACTGGCGACGATGGCGGTGTTTTGGTTCAGCG
CGCTGGCATTGTGGATTTATATCGCCAAGGAAAATTTCTTCCGCCCATTCGGACTGACGGCGAAATTCGGCAAACCGGAT
TGGGCGGTGTTCAAACAGATTTGGAAAATCGGCGCACCCATCGGGCTGTCTTATTTTTTGGAAGCCAGCGCGTTTTCGTT
TATCGTGTTTTTGATTGCGCCTTTCGGCGAGGATTATGTGGCGGCGCAGCAGGTCGGCATCAGTTTGTCGGGGATTCTCT
ATATGATTCCGCAAAGCGTCGGCTCGGCGGGGACGGTGCGCATCGGCTTTTCGCTTGGGCGGCGCGAATTTTCGCGGGCG
CGTTATATTTCGGGCGTGTCACTGGTGTTAGGATGGATGCTCGCCGTGATTACCGTGCTTTCCTTGGTATTATTCCGTTC
GCCGCTGGTAAGTATGTACAACAATGATCCGGCGGTTTTAAGCATCGCCGCCACCGTCTTACTGTTCGCCGGCTTGTTCC
AACCGGCAGACTTCACCCAATGTATCGCCTCCTACGCCTTGCGCGGCTACAAAGTTACAAAGGTGCCGATGTTCATCCAC
GCCGCCGCCTTTTGGGGCTGCGGTCTGCTGCCGGGCTACCTGCTCGCCTACCGTTTCGATATGGGCATTTACGGCTTCTG
GACGGCATTGATTGCCTCGCTCACCATCGCCGCCATCGCCTTGGTGTGGTGCTTGGAATTGTGCAGTAGGGAGATGGTCA
GATCGCATAAGGCCGTCTGA

Upstream 100 bases:

>100_bases
CGGCACGGCATTTTTTTACTATATAATGTGCGCCATTATTTCTCATCACTTCCTGCTGCCGTTTTCTGCTTCAGACGGCA
TTTTTGTTGATTGAACACTT

Downstream 100 bases:

>100_bases
AAACTCCATGGGTAGGATGAAGTCTGCTAATTACAATATTAATAGATGATGTGGCAGGGTTGAGCTAGGAGAATTTAGAG
TTTTTGAAGGGAGAGAGTTT

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVMAGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPM
IAQLYGAGKTDEVGETGRQGIWFGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALWIYIAKENFFRPFGLTAKFGKPD
WAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFGEDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRA
RYISGVSLVLGWMLAVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKVTKVPMFIH
AAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSREMVRSHKAV

Sequences:

>Translated_459_residues
MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVMAGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPM
IAQLYGAGKTDEVGETGRQGIWFGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALWIYIAKENFFRPFGLTAKFGKPD
WAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFGEDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRA
RYISGVSLVLGWMLAVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKVTKVPMFIH
AAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSREMVRSHKAV
>Mature_459_residues
MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVMAGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPM
IAQLYGAGKTDEVGETGRQGIWFGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALWIYIAKENFFRPFGLTAKFGKPD
WAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFGEDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRA
RYISGVSLVLGWMLAVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKVTKVPMFIH
AAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSREMVRSHKAV

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=446, Percent_Identity=25.5605381165919, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI153792564, Length=451, Percent_Identity=24.6119733924612, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI48994890, Length=442, Percent_Identity=35.7466063348416, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI226510954, Length=452, Percent_Identity=22.3451327433628, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320545, Length=428, Percent_Identity=24.5327102803738, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321821, Length=399, Percent_Identity=22.0551378446115, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49968; Mature: 49968

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]