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The map label for this gene is ponA [H]

Identifier: 161869413

GI number: 161869413

Start: 434976

End: 437306

Strand: Direct

Name: ponA [H]

Synonym: NMCC_0421

Alternate gene names: 161869413

Gene position: 434976-437306 (Clockwise)

Preceding gene: 161869398

Following gene: 161869415

Centisome position: 20.2

GC content: 52.12

Gene sequence:

>2331_bases
TTGGTTGCCATTGCTATTTTGGTAACGTATCCGAAACTGCCGTCTTTGGATTCTTTGCAGCATTACCAGCCTAAAATGCC
GTTGACTATTTATTCGGCGGATGGGGAAGTCATCGGTATGTATGGGGAGCAGCGGCGCGAATTTACAAAAATCGGCGATT
TCCCAGAGGTGTTGCGGAATGCGGTTATCGCCGCCGAGGACAAACGCTTTTACCGGCATTGGGGGGTGGATGTTTGGGGT
GTTGCCCGCGCTGCCGTCGGCAATGTCGTGTCCGGCAGCGTGCAGTCGGGTGCGAGTACGATTACGCAGCAGGTGGCGAA
AAATTTTTATTTGAGCAGTGAAAAAACGTTCACACGCAAATTCAATGAGGTGTTGCTTGCCTATAAAATCGAGCAGTCTT
TAAGCAAAGACAAAATCCTCGAGTTGTATTTCAATCAGATTTACCTCGGTCAGCGCGCCTATGGTTTTGCATCTGCCGCG
CAAATCTATTTCAATAAGAATGTCCGAGATTTGACTTTGGCGGAAGCCGCCATGCTTGCGGGACTGCCCAAGGCTCCGTC
TGCCTATAATCCGATTGTTAATCCAGAACGTGCCAAGTTGCGCCAGAAGTATATTTTGAACAATATGCTCGAGGAGAAGA
TGATTACCGTGCAACAGCGCGATCAGGCGTTGAATGAGGAACTGCATTACGAGCGGTTTGTTCGGAAAATCGATCAGAGT
GCTTTATATGTGGCGGAAATGGTGCGTCAGGAACTGTATGAGAAATACGGTGAAGATGCCTATACGCAGGGTTTTAAGGT
TTATACCACGGTCCGCGCCGATCATCAGAAGGTGGCAACCGAGGCATTGCGCAAGGCTCTACGGAATTTCGATCGCGGCA
GCAGCTACCGCGGTGCGGAAAACTATATCGATTTGAGTAAGAGTGAAGATGTCGAGGAGACTGTCAGCCAGTATCTGTCG
GGACTCTATACCGTCGATAAAATGGTTCCCGCCGTTGTGTTGGATGTGACTAAAAAGAAAAATGTCGTCATACAGCTGCC
CGGCGGCAGGCGGGTTACGCTTGACAGGCGCGCCTTGGGTTTTGCGGCCCGCGCGGTCAATAATGAAAAAATGGGGGAGG
ACCGTATCCGCAGGGGCGCGGTCATCCGTGTCAAAAACAACGGCGGGCGTTGGGCGGTGGTTCAAGAGCCGTTGCTGCAG
GGGGCTTTGGTTTCGCTGGATGCAAAAACCGGAGCTGTGCGCGCGCTGGTCGGCGGTTATGATTTTCACAGCAAAACATT
CAATCGTGCCGTTCAGGCAATGCGGCAGCCGGGTTCGACCTTTAAGCCGTTTGTCTATTCGGCGGCATTATCTAAGGGGA
TGACCGCGTCCACAGTGGTTAACGATGCGCCGATTTCCCTGCCGGGGAAAGGGCCGAACGGTTCGGTTTGGACACCTAAA
AATTCAGACGGCAGATATTCCGGCTACATTACTTTGAGACAGGCTCTGACGGCTTCCAAGAATATGGTTTCCATCCGTAT
TTTGATGTCTATCGGTGTCGGTTACGCGCAACAGTATATCCGGCGTTTCGGCTTCAGGTCGTCCGAGCTGCCGGCAAGCC
TGTCTATGGCTTTAGGTACGGGCGAGACAACGCCGTTGAAAGTGGCGGAGGCATATAGCGTATTTGCGAACGGCGGATAT
AGGGTTTCTTCGCACGTAATCGATAAGATTTATGACAGAGACGGCAGGTTGCGCGCCCAAATGCAACCTTTGGTGGCCGG
GCAAAATGCGCCTCAGGCAATCGATCCGCGCAATGCCTATATTATGTATAAGATTATGCAGGATGTGGTCCGTGTTGGTA
CGGCAAGGGGGGCAGCTGCGTTGGGAAGAACGGATATTGCCGGTAAAACGGGTACGACCAATGACAATAAGGATGCGTGG
TTTGTCGGTTTTAACCCTGATGTGGTTACTGCCGTATATATCGGCTTCGACAAACCTAAGAGTATGGGGCGTGTCGGCTA
CGGCGGTACGATTGCGGTGCCGGTTTGGGTGGACTATATGCGTTTTGCGTTGAAAGGAAAGCAGGGCAAGGGGATGAAAA
TGCCTGAAGGTGTGGTCAGCAGCAATGGCGAATACTATATGAAGGAACGTATGGTAACCGATCCGGGCTTGACGCTGGAC
AACAGCGGTATTGCGCCGCAACCTTCCCGACGGGCAAAAGAAGATGACGGGGGCGCGGCAGAAGGCGGACGGCAGGCGGC
GGATGACGAAGTCCGCCAAGATATGCAGGAAACGCCGGTGCTTCCGAGTAATACTGGTTCCAAACAGCAGCAGTTGGATT
CTCTGTTTTAA

Upstream 100 bases:

>100_bases
TTTAATACCAATAATGGTAAATTATTATTCAGCTATGATTAAAAAGATTTTAACGACTTGTTTTGGTTTGGTTTTTGGGT
TTTGTGTATTTGGAGTGGGT

Downstream 100 bases:

>100_bases
AGACTCCGCAAAATGCCGTCTGAAAAGTCTTTCAGACGGCATTTTAGATTTGGCAGTGGCAATTTTTTAAATGTTTGCGG
TCGGTCAAGTGGGGGGAATA

Product: penicillin-binding protein 1

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 776; Mature: 776

Protein sequence:

>776_residues
MVAIAILVTYPKLPSLDSLQHYQPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDKILELYFNQIYLGQRAYGFASAA
QIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPERAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQS
ALYVAEMVRQELYEKYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVEETVSQYLS
GLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEKMGEDRIRRGAVIRVKNNGGRWAVVQEPLLQ
GALVSLDAKTGAVRALVGGYDFHSKTFNRAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPK
NSDGRYSGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETTPLKVAEAYSVFANGGY
RVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIMYKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAW
FVGFNPDVVTAVYIGFDKPKSMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLTLD
NSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF

Sequences:

>Translated_776_residues
MVAIAILVTYPKLPSLDSLQHYQPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDKILELYFNQIYLGQRAYGFASAA
QIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPERAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQS
ALYVAEMVRQELYEKYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVEETVSQYLS
GLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEKMGEDRIRRGAVIRVKNNGGRWAVVQEPLLQ
GALVSLDAKTGAVRALVGGYDFHSKTFNRAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPK
NSDGRYSGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETTPLKVAEAYSVFANGGY
RVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIMYKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAW
FVGFNPDVVTAVYIGFDKPKSMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLTLD
NSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
>Mature_776_residues
MVAIAILVTYPKLPSLDSLQHYQPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDKILELYFNQIYLGQRAYGFASAA
QIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPERAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQS
ALYVAEMVRQELYEKYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVEETVSQYLS
GLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEKMGEDRIRRGAVIRVKNNGGRWAVVQEPLLQ
GALVSLDAKTGAVRALVGGYDFHSKTFNRAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPK
NSDGRYSGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETTPLKVAEAYSVFANGGY
RVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIMYKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAW
FVGFNPDVVTAVYIGFDKPKSMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLTLD
NSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=583, Percent_Identity=43.2246998284734, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786343, Length=280, Percent_Identity=36.0714285714286, Blast_Score=157, Evalue=4e-39,
Organism=Escherichia coli, GI1788867, Length=188, Percent_Identity=36.1702127659575, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789601, Length=151, Percent_Identity=39.0728476821192, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 85804; Mature: 85804

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAIAILVTYPKLPSLDSLQHYQPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRN
CEEEEEEEECCCCCCCCCHHHCCCCCCEEEEECCCCEEECCCHHHHHHHHCCCHHHHHHH
AVIAAEDKRFYRHWGVDVWGVARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
FNEVLLAYKIEQSLSKDKILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHCCCHHHEEEEECCCCCHHHHHHHHHHH
GLPKAPSAYNPIVNPERAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALYVAEMVRQELYEKYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAE
HHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCC
NYIDLSKSEDVEETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCEEEECHHHHH
FAARAVNNEKMGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGY
HHHHHCCCCCCCHHHHHCCCEEEEECCCCEEEEECCHHHCCCEEEECCCCCHHHHHHCCC
DFHSKTFNRAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPK
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHEECCCCCCCCCCCCCCCEECCC
NSDGRYSGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGT
CCCCCEEEEEEHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHHHCCCCCCCCCHHEEEECC
GETTPLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAY
CCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCE
IMYKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
EHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCC
SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLTLD
CCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCEEEECCEECCCCCEEC
NSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MVAIAILVTYPKLPSLDSLQHYQPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRN
CEEEEEEEECCCCCCCCCHHHCCCCCCEEEEECCCCEEECCCHHHHHHHHCCCHHHHHHH
AVIAAEDKRFYRHWGVDVWGVARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
FNEVLLAYKIEQSLSKDKILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHCCCHHHEEEEECCCCCHHHHHHHHHHH
GLPKAPSAYNPIVNPERAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALYVAEMVRQELYEKYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAE
HHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCC
NYIDLSKSEDVEETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCEEEECHHHHH
FAARAVNNEKMGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGY
HHHHHCCCCCCCHHHHHCCCEEEEECCCCEEEEECCHHHCCCEEEECCCCCHHHHHHCCC
DFHSKTFNRAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPK
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHEECCCCCCCCCCCCCCCEECCC
NSDGRYSGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGT
CCCCCEEEEEEHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHHHCCCCCCCCCHHEEEECC
GETTPLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAY
CCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCE
IMYKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
EHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCC
SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLTLD
CCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCEEEECCEECCCCCEEC
NSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA